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Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
ACTA University of Amsterdam, Netherlands; Vrije University of Amsterdam, Netherlands.
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2015 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 10, no 7, e0134023Article in journal (Refereed) Published
Abstract [en]

Polypyrrole (PPy) is a conducting polymer that enables controlled drug release upon electrical stimulation. We characterized the biocompatibility of PPy with human primary osteoblasts, and the effect of dopants. We investigated the biocompatibility of PPy comprising various dopants, i.e. p-toluene sulfonate (PPy-pTS), chondroitin sulfate (PPy-CS), or dodecylbenzenesulfonate (PPy-DBS), with human primary osteoblasts. PPy-DBS showed the roughest appearance of all surfaces tested, and its wettability was similar to the gold-coated control. The average number of attached cells was 45% higher on PPy-DBS than on PPyCS or PPy-pTS, although gene expression of the proliferation marker Ki-67 was similar in osteoblasts on all surfaces tested. Osteoblasts seeded on PPy-DBS or gold showed similar vinculin attachment points, vinculin area per cell area, actin filament structure, and Ferets diameter, while cells seeded on PPY-CS or PPY-pTS showed disturbed focal adhesions and were enlarged with disorganized actin filaments. Osteoblasts grown on PPy-DBS or gold showed enhanced alkaline phosphatase activity and osteocalcin gene expression, but reduced osteopontin gene expression compared to cells grown on PPy-pTS and PPy-CS. In conclusion, PPy doped with DBS showed excellent biocompatibility, which resulted in maintaining focal adhesions, cell morphology, cell number, alkaline phosphatase activity, and osteocalcin gene expression. Taken together, conducting polymers doped with DBS are well tolerated by osteoblasts. Our results could provide a basis for the development of novel orthopedic or dental implants with controlled release of antibiotics and pharmaceutics that fight infections or focally enhance bone formation in a tightly controlled manner.

Place, publisher, year, edition, pages
Public Library of Science , 2015. Vol. 10, no 7, e0134023
National Category
Cell and Molecular Biology
URN: urn:nbn:se:liu:diva-120873DOI: 10.1371/journal.pone.0134023ISI: 000358837700051PubMedID: 26225862OAI: diva2:849402

Funding Agencies|VINNOVA [2012-04409]; Vetenskapradet [521-2013-2593]; Linkoping Initiative in Life Science Technologies (LIST)

Available from: 2015-08-28 Created: 2015-08-28 Last updated: 2015-09-17

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Fahlgren, AnnaBratengeier, CorneliaJager, Edwin
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Division of Cell BiologyFaculty of Medicine and Health SciencesBiosensors and BioelectronicsFaculty of Science & Engineering
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