liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
A panel of PCR-inhibitory reference materials for quality evaluation of multiplex STR analysis kits: -
Show others and affiliations
2015 (English)In: Abstracts ISFG, 2015, 226- p.Conference paper, Abstract (Refereed)
Abstract [en]

PCR inhibition is a key challenge in forensic DNA analysis. Substances interfering with the amplification of PCR products can lower the success rate of Short Tandem Repeat (STR) analysis or generate ambiguous DNA profiles.

For forensic DNA laboratories it is therefore vital to have knowledge about how common inhibitory substances affect their STR analysis system. We have developed a broad-range panel of standardized PCR-inhibitory reference materials (RMs), representing the heterogeneous stains found at crime scenes. The panel, including solutions prepared from for example cigarette paper,

chewing gum, moist snuff and humic acid, is a tool for quality evaluation of STR systems. We applied the RMs to challenge PowerPlex® ESX 16 Fast System (ESX Fast). Although ESX Fast tolerated high levels of some inhibitors, several RMs caused problems in different ways. Humic acid had a specific negative effect on amelogenin amplification, moist snuff hindered amplification of longer fragments, and chewing gum caused generally lowered allele peak heights. These different effects may provide information regarding the mechanisms of inhibition. For instance, our results indicate that one effect of humic acid on ESX Fast analysis is chelation of Mg2+ ions, thus altering the melting temperatures of the primers. In the developmental validation of STR systems, a limited evaluation of PCR inhibition involving only a few substances is generally performed. Applying a broad panel of RMs will ensure that a wider range of inhibitory substances from crime scene samples are included, giving a better understanding of inhibitor tolerance and effects.

 

Place, publisher, year, edition, pages
2015. 226- p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-120901OAI: oai:DiVA.org:liu-120901DiVA: diva2:849426
Conference
26th Congress of the International Society for Forensic Genetics
Available from: 2015-08-28 Created: 2015-08-28 Last updated: 2015-09-17

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Ansell, Ricky
By organisation
BiologyFaculty of Science & Engineering
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 56 hits
ReferencesLink to record
Permanent link

Direct link