Studies on an on/off-switchable immunosensor for troponin T
2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73, 100-107 p.Article in journal (Refereed) Published
Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (Delta G(coil)=-6.0 Kcal/mole) and 37 degrees C (Delta G(globular)=-41.0 kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with built-in programmable interactions of antigen-antibody upon receiving stimuli.
Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 73, 100-107 p.
On/off-switching; Troponin T; Programmable bioelectronics; Smart immunosensors
Physical Chemistry Materials Chemistry
IdentifiersURN: urn:nbn:se:liu:diva-120853DOI: 10.1016/j.bios.2015.05.055ISI: 000358823900014PubMedID: 26056953OAI: oai:DiVA.org:liu-120853DiVA: diva2:849483
Funding Agencies|Swedish Institute [00037/2014]; Initiative in Life Science Technologies (LIST); Linkoping University2015-08-282015-08-282015-09-17