liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Studies on an on/off-switchable immunosensor for troponin T
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Dhaka, Bangladesh.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
Royal Institute Technology, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
Show others and affiliations
2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73, 100-107 p.Article in journal (Refereed) Published
Abstract [en]

Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (Delta G(coil)=-6.0 Kcal/mole) and 37 degrees C (Delta G(globular)=-41.0 kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with built-in programmable interactions of antigen-antibody upon receiving stimuli.

Place, publisher, year, edition, pages
Elsevier , 2015. Vol. 73, 100-107 p.
Keyword [en]
On/off-switching; Troponin T; Programmable bioelectronics; Smart immunosensors
National Category
Physical Chemistry Materials Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-120853DOI: 10.1016/j.bios.2015.05.055ISI: 000358823900014PubMedID: 26056953OAI: oai:DiVA.org:liu-120853DiVA: diva2:849483
Note

Funding Agencies|Swedish Institute [00037/2014]; Initiative in Life Science Technologies (LIST); Linkoping University

Available from: 2015-08-28 Created: 2015-08-28 Last updated: 2015-09-17

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Turner, AnthonyTiwari, Ashutosh
By organisation
Biosensors and BioelectronicsFaculty of Science & Engineering
In the same journal
Biosensors & bioelectronics
Physical ChemistryMaterials Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 202 hits
ReferencesLink to record
Permanent link

Direct link