Drosophila olfactory sensory neurons have two phases of gene expression regulation
(English)Manuscript (preprint) (Other academic)
Here, we investigate the gene regulatory mechanisms that buffer environmental challenges and are required for Drosophila OSNs to maintain their fate. Each OSN expresses one odorant receptor (OR) gene from a large OR gene repertoire and the expression is maintained throughout the life of the neuron. We demonstrate that OSNs transit from a permissive gene regulatory state at the end of development to a robust continuous regulatory state in mature OSNs that secure the expression of a single OR gene. We provide evidence that the switch is associated to a change in the H3K9 methylation state. We show that the H3K9 demetylase su(var)3-3 is required for the permissive phase and the robust phase require the H3K9 methylase, su(var) 3-9. We further show that H3K9 methylation status has a role in the regulation of gene expression during the environmental challenges. Thus, our data demonstrate that OSNs go through two separate phases that compensate the environmental fluctuations differently.
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-120991OAI: oai:DiVA.org:liu-120991DiVA: diva2:850472