Tuning the aqueous self-assembly process of insulin by a hydrophobic additive
2015 (English)In: RSC ADVANCES, ISSN 2046-2069, Vol. 5, no 112, 92254-92262 p.Article in journal (Refereed) Published
Biomolecular self-assembly is an efficient way of preparing soft-matter based materials. Herein we report a novel method, based on the use of insoluble additives in aqueous media, for influencing the self-assembly process. Due to their low solubility, the use of hydrophobic additives in aqueous media is problematic; however, by mixing the additive with the biomolecule in the solid state, prior to solvation, this problem can be circumvented. In the investigated self-assembly system, where bovine insulin self-assembles into spherical structures, the inclusion of the hydrophobic material α-sexithiophene (6T) results in significant changes in the self-assembly process. Under our reaction conditions, in the case of materials prepared from insulin-only the growth of spherulites typically stops at a diameter of 150μm. However, by adding 2 weight % of hydrophobic material, spherulite growth continues up to diameters in the mm-range. The spherulites incorporate 6T and are thus fluorescent. The method reported herein should be of interest to all scientists working in the field of self-assembly as the flexible materials preparation, based simply on co-grinding of commercially available materials, adds another option to influence the structure and properties of products formed by self-assembly reactions.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015. Vol. 5, no 112, 92254-92262 p.
Organic Chemistry Biomaterials Science
IdentifiersURN: urn:nbn:se:liu:diva-121018DOI: 10.1039/c5ra16144dISI: 000364032500040OAI: oai:DiVA.org:liu-121018DiVA: diva2:850847
Funding agencies: Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linkoping University (Faculty Grant SFO-Mat-LiU) [2009-00971]; Knut and Alice Wallenberg foundation2015-09-022015-09-022015-12-03