NUCLEOSIDE ANALOG ACTIVITY IN MALIGNANT MELANOMA CELL LINES
2015 (English)In: Nucleosides, Nucleotides & Nucleic Acids, ISSN 1525-7770, E-ISSN 1532-2335, Vol. 34, no 9, 639-649 p.Article in journal (Refereed) Published
Mitochondrial deoxyguanosine kinase (dGK), is an enzyme responsible for activation of nucleoside analogs (NAs) to phosphorylated compounds which exert profound cytotoxicity, especially in hematological malignancies. Screening malignant melanoma cell lines against NAs revealed high sensitivity to several of them. This was believed to be due to the high levels of dGK expression in these cells. Downregulation of dGK in the melanoma cell line RaH5 using siRNA did not cause resistance to NAs as expected, but instead cells became more sensitive. This was probably partly due to the increased activity of another mitochondrial enzyme, thymidine kinase 2, seen in transfected cells.
Place, publisher, year, edition, pages
Taylor andamp; Francis: STM, Behavioural Science and Public Health Titles , 2015. Vol. 34, no 9, 639-649 p.
Deoxyguanosine kinase; melanoma; leukemia; nucleoside analog; RNAi
IdentifiersURN: urn:nbn:se:liu:diva-121095DOI: 10.1080/15257770.2015.1047029ISI: 000359601400004PubMedID: 26252632OAI: oai:DiVA.org:liu-121095DiVA: diva2:852016
Funding Agencies|Swedish Cancer Foundation; County Council of Ostergotland; Swedish Medical Society; AFA Insurance; Swedish Fund for Research without Animal Experiments2015-09-072015-09-072016-03-23