Modeling Perfusion Dynamics in the Skin During Iontophoresis of Vasoactive Drugs Using Single-Pulse and Multiple-Pulse Protocols
2015 (English)In: Microcirculation, ISSN 1073-9688, E-ISSN 1549-8719, Vol. 22, no 6, 446-453 p.Article in journal (Refereed) Published
Objective: After iontophoresis of vasoactive drugs into the skin, a decrease in perfusion is commonly observed. We delivered vasoactive drugs by iontophoresis using different delivery protocols to study how these affect this decrease in perfusion as measured using LDF. Methods: We measured skin perfusion during iontophoresis of (ACh), MCh, andNAusing a single pulse or separate pulses at different skin sites, and during repeated delivery of ACh at the same site. Results: Perfusion half-life was 6.1 (5.6-6.6) minutes for ACh and 41 (29-69) minutes for MCh (p less than 0.001). The maximum response with multiple pulses of ACh iontophoresis was lower than with a single pulse, 30 (22-37) PU vs. 43 (36-50) PU, p less than 0.001. Vasoconstriction to NA was more rapid with a single pulse than with multiple pulses. The perfusion half-life of ACh decreased with repeated delivery of ACh at the same site-first 16 (14-18), second 5.9 (5.1-6-9) and third 3.2 (2.9-3.5) minutes, p less than 0.001. Conclusions: The drug delivery protocol affects microvascular responses to iontophoresis, possibly as a result of differences in the dynamics of local drug concentrations. Perfusion half-life may be used as a measure to quantify the rate of perfusion recovery after iontophoresis of vasoactive drugs.
Place, publisher, year, edition, pages
Informa Healthcare / Wiley: 12 months , 2015. Vol. 22, no 6, 446-453 p.
microcirculation; iontophoresis; acetylcholine; metha choline; noradrenaline; skin
IdentifiersURN: urn:nbn:se:liu:diva-121138DOI: 10.1111/micc.12211ISI: 000359676500002PubMedID: 26016387OAI: oai:DiVA.org:liu-121138DiVA: diva2:852279