A series of Zn(II) terpyridine complexes with enhanced two-photon-excited fluorescence for in vitro and in vivo bioimaging
2015 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 3, no 36, 7213-7221 p.Article in journal (Refereed) Published
It is still a challenge to obtain two-photon excited fluorescent bioimaging probes with intense emission, high photo-stability and low cytotoxicity. In the present work, four Zn(II)-coordinated complexes (1-4) constructed from two novel D-A and D-p-A ligands (L-1 and L-2) are investigated both experimentally and theoretically, aiming to explore efficient two-photon probes for bioimaging. Molecular geometry optimization used for theoretical calculations is achieved using the crystallographic data. Notably, the results indicate that complexes 1 and 2 display enhanced two-photon absorption (2PA) cross sections compared to their corresponding D-A ligand (L1). Furthermore, it was found that complex 1 has the advantages of moderate 2PA cross section in the near-infrared region, longer fluorescence lifetime, higher quantum yield, good biocompatibility and enhanced two-photon excited fluorescence. Therefore, complex 1 is evaluated as a bioimaging probe for in vitro imaging of HepG2 cells, in which it is observed under a two-photon scanning microscope that complex 1 exhibits effective co-staining with endoplasmic reticulum (ER) and nuclear membrane; as well as for in vivo imaging of zebrafish larva, in which it is observed that complex 1 exhibits specificity in the intestinal system.
Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY , 2015. Vol. 3, no 36, 7213-7221 p.
IdentifiersURN: urn:nbn:se:liu:diva-122077DOI: 10.1039/c5tb01185jISI: 000360658000007OAI: oai:DiVA.org:liu-122077DiVA: diva2:862048
Funding Agencies|National Natural Science Foundation of China [51372003, 21271004, 51272001, 51472003, 51432001, 21271003]; Ministry of Education Funded Projects Focus on returned overseas scholar; Program for New Century Excellent Talents in University (China); Doctoral Program Foundation of Ministry of Education of China ; China Postdoctoral Science Foundation [2015M571912]; Swedish Research Council (VR) [621-2013-5357]; Swedish Government strategic faculty grant in materials science (SFO, MATLIU) in Advanced Functional Materials (AFM)2015-10-202015-10-192015-10-28