Regulatory T-cell Subpopulations in Severe or Early-onset Preeclampsia
2015 (English)In: American Journal of Reproductive Immunology, ISSN 1046-7408, E-ISSN 1600-0897, Vol. 74, no 4, 368-378 p.Article in journal (Refereed) Published
Problem A deficiency in regulatory T (Treg) cells causing reduced immune regulatory capacity has been proposed in preeclampsia. Objective Utilizing recent advances in flow cytometry phenotyping, we aimed to assess whether a deficiency of Treg subpopulations occurs in preeclampsia. Method of study Six-color flow cytometry was used for Treg phenotyping in 18 preeclamptic women (one early-onset, one severe and 16 both), 20 women with normal pregnancy, and 20 non-pregnant controls. Results No differences were found in major Treg populations including CD127(low)CD25(+)/CD127(ow)FOXP3(+), resting (FOXP3(dim)CD45RA(+)), and activated (FOXP3(bright)CD45RA(-)) Treg cells, whereas preeclamptic women showed increased CTLA-4(+) and CCR4(+) proportions within resting/activated Treg populations. Corticosteroid treatment prior to blood sampling (n = 10) affected the distribution of Treg populations. Conclusions Although we found no major alterations in circulating Treg frequencies, differences in CTLA-4(+) and CCR4(+) frequencies suggest a migratory defect of Treg cells in preeclampsia. Corticosteroid treatment should be taken into account when evaluating Treg cells.
Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2015. Vol. 74, no 4, 368-378 p.
Early-onset preeclampsia; preeclampsia; pregnancy; regulatory T cells
Obstetrics, Gynecology and Reproductive Medicine
IdentifiersURN: urn:nbn:se:liu:diva-122528DOI: 10.1111/aji.12410ISI: 000362664200009PubMedID: 26118401OAI: oai:DiVA.org:liu-122528DiVA: diva2:868016
Funding Agencies|FORSS (Medical Research Council of Southeast Sweden); Futurum, academy for Health and Care Jonkoping County Council, Sweden2015-11-092015-11-062016-03-24