Dual-modal CT and MRI functional and anatomical imaging using barium sulphate and gadolinium nanoparticle loaded macrophages in a preclinical asthma mouse model
2015 (English)Manuscript (preprint) (Other academic)
Objectives In this study we investigated the potentials of dual-modal CT-MRI macrophage tracking, by a intratracheal instillation of a mixture of either gadolinium nanoparticles or barium sulphate loaded alveolar macrophages into mice of an allergic airway inflammation (asthma) model and their respective healthy control, imaged with Synchrotron X-rays microtomography (SR μCT) and Micro Magnetic Resonance Imaging (μMRI).
Materials and Methods The mice were scanned ex vivo using SRμCT at 22 keV and with a 9.4 Tesla μMRI scanner. The CT and MRI data sets were registered and fused together, followed by quantitative and statistical analysis.
Results The asthmatic sample injected with contrast agent loaded macrophages showed high absorbing spots inside the soft-tissue regions of the lung for the CT data set, as well as higher contrast for the soft-tissue in the MRI data set. Furthermore, the correlation analysis showed a perfect negative correlation between the soft tissue mean grey value in CT and the soft tissue mean grey value in MRI.
Conclusion The dual-modal CT-MRI cell tracking of intratracheally administered macrophages (loaded with contrast agent) in an asthmatic mouse helps to extract synergistic information about the migration behaviour of macrophages, where clusters of cells were detected in CT, while as a general increase of the soft-tissue contrast could be observed in MRI, due to a homogeneous cell distribution.
Place, publisher, year, edition, pages
Gadolinium oxide (Gd2O3); alveolar macrophages; asthma; magnetic resonance imaging; Synchrotron X-Ray Computed Microtomography
Physical Sciences Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:liu:diva-122603OAI: oai:DiVA.org:liu-122603DiVA: diva2:868592