liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
405 nm versus 633 nm for protoporphyrin IX excitation in fluorescence-guided stereotactic biopsy of brain tumors
Ludwig Maximilians University in Munich.
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, Faculty of Science & Engineering. (MINT)ORCID iD: 0000-0002-0555-8877
Ludwig Maximilians University in Munich.
Ludwig Maximilians University in Munich.
Show others and affiliations
2015 (English)In: Journal of Biophotonics, ISSN 1864-063X, E-ISSN 1864-0648Article in journal (Refereed) Epub ahead of print
Abstract [en]

Fluorescence diagnosis may be used to improve the safety and reliability of stereotactic brain tumor biopsies using biopsy needles with integrated fiber optics. Based on 5-aminolevulinic-acid-induced protoporphyrin IX (PpIX) fluorescence, vital tumor tissue can be localized in vivo during the excision procedure to reduce the number of necessary samples for a reliable diagnosis. In this study, the practical suitability of two different PpIX excitation wavelengths (405 nm, 633 nm) was investigated on optical phantoms. Violet excitation at 405 nm provides a 50-fold higher sensitivity for the bulk tumor; this factor increases up to 100 with decreasing fluorescent volume as shown by ray tracing simulations. Red excitation at 633 nm, however, is noticeably superior with regard to blood layers obscuring the fluorescence. Experimental results on the signal attenuation through blood layers of well-defined thicknesses could be confirmed by ray tracing simulations. Typical interstitial fiber probe measurements were mimicked on agarose-gel phantoms. Even in direct contact, blood layers of 20-40 µm between probe and tissue must be expected, obscuring 405-nm-excited PpIX fluorescence almost completely, but reducing the 633-nm-excited signal only by 25.5%. Thus, 633 nm seems to be the wavelength of choice for PpIX-assisted detection of high-grade gliomas in stereotactic biopsy. PpIX signal attenuation through clinically relevant blood layers for 405 nm (violet) and 633 nm (red) excitation.

Place, publisher, year, edition, pages
Keyword [en]
5-aminolevulinic acid;protoporphyrin IX;stereotactic biopsy;fluorescence spectroscopy;glioblastoma multiforme
National Category
Atom and Molecular Physics and Optics
URN: urn:nbn:se:liu:diva-122640DOI: 10.1002/jbio.201500195PubMedID: 26564058OAI: diva2:871427
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-08-31

Open Access in DiVA

fulltext(809 kB)111 downloads
File information
File name FULLTEXT01.pdfFile size 809 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Haj-Hosseini, Neda
By organisation
Biomedical InstrumentationFaculty of Science & Engineering
In the same journal
Journal of Biophotonics
Atom and Molecular Physics and Optics

Search outside of DiVA

GoogleGoogle Scholar
Total: 111 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 234 hits
ReferencesLink to record
Permanent link

Direct link