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Tissue specific expression of extracellular microRNA in human breast cancers and normal human breast tissue in vivo
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Oncology.
2015 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 6, no 26, 22959-22969 p.Article in journal (Refereed) Published
Abstract [en]

Extracellular circulating microRNAs (miRNAs) have been suggested to be biomarkers for disease monitoring but data are inconsistent, one reason being that blood miRNA is of heterogeneous origin. Here, we sampled extracellular microRNAs locally in situ using microdialysis. Three different cohorts of women were included; postmenopausal women with ongoing breast cancer investigated within the cancer and in normal adjacent breast tissue, postmenopausal women investigated in their normal healthy breast and subcutaneous fat before and after six weeks of tamoxifen therapy, premenopausal women during the menstrual cycle. Samples were initially screened using TaqMan array cards with subsequently absolute quantification. 124 miRNA were expressed in microdialysates. After absolute quantifications extracellular miRNA-21 was found to be significantly increased in breast cancer. In addition, the levels were significantly higher in pre-menopausal breast tissue compared with postmenopausal. In breast tissue of pre-menopausal women miRNA-21 exhibited a cyclic variation during the menstrual cycle and in postmenopausal women six weeks of tamoxifen treatment decreased miRNA-21 suggesting that this miRNA may be important for breast carcinogenesis. None of these changes were found in plasma or microdialysates from subcutaneous fat. Our data revealed tissue specific changes of extracellular circulating miRNAs that would be otherwise unraveled using blood samples.

Place, publisher, year, edition, pages
Albany, NY, United States: Impact Journals LLC , 2015. Vol. 6, no 26, 22959-22969 p.
Keyword [en]
mammary gland; microdialysis; sex steroids; estrogen; tamoxifen
National Category
Clinical Medicine
URN: urn:nbn:se:liu:diva-122668DOI: 10.18632/oncotarget.4038ISI: 000362954800106OAI: diva2:871692

Funding Agencies|Swedish Cancer Society [2012/454]; Swedish Research Council [2013-2457]; Linkoping University

Available from: 2015-11-16 Created: 2015-11-13 Last updated: 2015-12-10

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Abrahamsson, AnnelieDabrosin, Charlotta
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