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Urine oligosaccharide pattern in patients with hyperprolactinaemia
Region Östergötland, Heart and Medicine Center, Department of Endocrinology. Linköping University, Faculty of Medicine and Health Sciences. Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine.
Region Östergötland, Heart and Medicine Center, Department of Endocrinology. Linköping University, Faculty of Medicine and Health Sciences. Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry. Linköping University, Faculty of Medicine and Health Sciences.
2015 (English)In: Glycoconjugate Journal, ISSN 0282-0080, E-ISSN 1573-4986, Vol. 32, no 8, 635-641 p.Article in journal (Refereed) Published
Abstract [en]

Free milk-type oligosaccharides are produced during pregnancy and lactation and may have an impact on several cells in the immune system. Our aim was to investigate if patients with isolated hyperprolactinaemia, not related to pregnancy, also have increased synthesis and urinary excretion of milk-type oligosaccharides and to compare the excretion pattern with that found during pregnancy. Urine samples were collected as morning sample from 18 patients with hyperprolactinaemia, 13 healthy controls with normal prolactin levels and four pregnant women. After purification, lactose and free oligosaccharides were analysed and quantified by high-performance anion-exchange chromatography with pulsed amperometric detection. The identity of peaks was confirmed by exoglycosidase treatment and comparison with oligosaccharide standards. Prolactin was measured in serum collected between 09 and 11 a.m. by a standardized immunochemical method. Patients with hyperprolactinaemia had higher urinary excretion of lactose than normoprolactinemic controls and urinary lactose correlated positively to prolactin levels (r = 0.51, p less than 0.05). Increased levels of the fucosylated oligosaccharides 2-fucosyl lactose and lacto-di-fucotetraose were found in urine from three and two patients, respectively. The acidic oligosaccharide 3-sialyl lactose was found in high amount in urine from two patients with prolactin of greater than 10,000 mU/l. However, pregnant women in their third trimester had the highest concentration of all these oligosaccharides and excretion increased during pregnancy. This study is first to show that both lactose and certain fucosylated and sialylated milk-type oligosaccharides are increased in some patients with hyperprolactinaemia. It remains to elucidate the functional importance of these findings.

Place, publisher, year, edition, pages
SPRINGER , 2015. Vol. 32, no 8, 635-641 p.
Keyword [en]
Prolactin; Prolactinoma; Urine; Oligosaccharides; Lactose
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-122650DOI: 10.1007/s10719-015-9610-xISI: 000363488300006PubMedID: 26275984OAI: oai:DiVA.org:liu-122650DiVA: diva2:871746
Note

Funding Agencies|FORSS (Medical Research Council of Southeast Sweden) [4065]; Faculty of Health and Sciences, Linkoping University, Sweden

Available from: 2015-11-16 Created: 2015-11-13 Last updated: 2016-04-25

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Ekman, BertilWahlberg Topp, JeanetteLandberg, Eva
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Department of EndocrinologyFaculty of Medicine and Health SciencesDivision of Cardiovascular MedicineDivision of Cell BiologyDepartment of Clinical Chemistry
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Glycoconjugate Journal
Clinical Medicine

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