liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal´s lab)
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
The Bioinformatics Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-3894-2218
2015 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 15, 4021-4029 p.Article in journal (Refereed) Published
Abstract [en]

The marine mussel innate immunity provides protection to pathogen invasion and inflammation.In this regard, the mussel hemolymph takes a main role in the animal innate response.Despite the importance of this body fluid in determining the physiological condition of theanimal, little is known about the molecular mechanisms underlying the cellular and humoralresponses. In this work, we have applied aMS (nano-LC-MS/MS) strategy integrating genomicand transcriptomic data with the aim to: (i) identify the main protein functional groups thatcharacterize hemolymph and (ii) to map the elements of innate immunity in the marine musselMytilus edulis hemolymph proteome. After sample analysis and first protein identificationbased onMS/MS data comparison, proteins with unknown functions were annotated with blastusing public database (nrNCBI) information. Overall 595 hemolymph proteins were identifiedwith high confidence and annotated. These proteins encompass primary cellular metabolicprocesses: energy production and metabolism of biomolecules, as well as processes related tooxidative stress defence, xenobiotic detoxification, drug metabolism, and immune response.A group of proteins was identified with putative immune effector, receptor, and signalingfunctions in M. edulis. Data are available via ProteomeXchange with identifier PXD001951(http://proteomecentral.proteomexchange.org/dataset/PXD001951).

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015. Vol. 15, 4021-4029 p.
Keyword [en]
Animal proteomics / Biomarker / Hemolymph / Innate immunity / Mytilus edulis / Shotgun proteomics
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-123354DOI: 10.1002/pmic.201500118PubMedID: 26351202OAI: oai:DiVA.org:liu-123354DiVA: diva2:881957
Available from: 2015-12-12 Created: 2015-12-12 Last updated: 2016-04-29Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2016-10-13 15:09
Available from 2016-10-13 15:09

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Alexandre, CamposCristobal, Susana
By organisation
Division of Cell BiologyFaculty of Medicine and Health Sciences
In the same journal
Proteomics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 267 hits
ReferencesLink to record
Permanent link

Direct link