Surface-Engineered Contact Lens as an Advanced Theranostic Platform for Modulation and Detection of Viral Infection
2015 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, no 45, 25487-25494 p.Article in journal (Refereed) PublishedText
We have demonstrated an entirely new concept of a wearable theranostic device in the form of a contact lens (theranostic lens) with a dual-functional hybrid surface to modulate and detect a pathogenic attack, using a the corneal HSV serotype-1 (HSV-1) model. The theranostic lenses were constructed using a facile layer-by-layer surface engineering technique, keeping the theranostic lenses with good surface wettability, optically transparency, and nontoxic toward human corneal epithelial cells. The theranostic lenses were used to capture and concentrate inflammatory cytokines such as interleukin-1 alpha (IL-1 alpha), which is upregulated during HSV-1 reactivation, for sensitive, noninvasive diagnostics. The theranostic lens also incorporated an antiviral coating to serve as a first line of defense to protect patients against disease. Our strategy tackles major problems in tear diagnostics that are mainly associated with the sampling of a relatively small volume of fluid and the low concentration of biomarkers. The theranostic lenses show effective anti-HSV-1 activity and good analytical performance for the detection of IL-1a, with a limit of detection of 1.43 pg mL(-1) and a wide linear range covering the clinically relevant region. This work offers a new paradigm for wearable noninvasive healthcare devices combining diagnosis and protection against disease, while supporting patient compliance. We believe that this approach holds immense promise as a next-generation point-of-care and decentralized diagnostic/theranostic platform for a range of biomarkers.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2015. Vol. 7, no 45, 25487-25494 p.
theranostics; contact lens; cornea; antiviral; diagnostics
Biological Sciences Clinical Medicine Physical Sciences
IdentifiersURN: urn:nbn:se:liu:diva-123520DOI: 10.1021/acsami.5b08644ISI: 000365148600060PubMedID: 26512953OAI: oai:DiVA.org:liu-123520DiVA: diva2:886283
Funding Agencies|Linkoping Center for Life Science Technologies; Swedish Research Council [521-2012-5706]2015-12-222015-12-212016-01-11