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Controlled delivery of human cells by temperature responsive microcapsules
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University.
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2015 (English)In: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 6, no 2, 439-453 p.Article in journal (Refereed) Published
Abstract [en]

Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line—human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

Place, publisher, year, edition, pages
2015. Vol. 6, no 2, 439-453 p.
Keyword [en]
cell encapsulation; microcapsules; hydrogel; cell delivery; temperature responsive; human fibroblast; human umbilical vein endothelial cells
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-123654DOI: 10.3390/jfb6020439OAI: oai:DiVA.org:liu-123654DiVA: diva2:890984
Available from: 2016-01-05 Created: 2016-01-05 Last updated: 2017-12-01

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Olesen, KimSivlér, PetterEdin, JoelSkog, MårtenGriffith, May

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Olesen, KimSivlér, PetterEdin, JoelSkog, MårtenGriffith, May
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