liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
A panel of PCR-inhibitory reference materials for quality evaluation of multiplex STR analysis kits
Swedish National Forensic Centre, Linköping, Sweden.
Swedish National Forensic Centre, Linköping, Sweden.
Swedish National Forensic Centre, Linköping, Sweden.
Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden.
Show others and affiliations
2015 (English)In: Forensic Science International: Genetics Supplement Series, ISSN 1875-1768, E-ISSN 1875-175X, Vol. 5, e317-319 p.Article in journal (Refereed) Published
Abstract [en]

PCR inhibition is a critical parameter in forensic DNA analysis. Substances interfering with amplification of short tandem repeats (STRs) may generate partial and/or ambiguous DNA profiles. We present a strategy for developing a broad panel of PCR-inhibitory reference materials (RMs), representing common casework samples. Our panel, including solutions prepared from for example cigarettes, chewing gum and soil, is a tool for in-house validation and lot testing of STR systems. PowerPlex ESX 16 Fast System tolerated high levels of some RMs, but several substances caused amplification problems. Humic acid had a negative effect on amelogenin, moist snuff hindered amplification of longer fragments, and chewing gum caused generally lowered allele peak heights. Applying a broad panel of RMs ensures that a wide range of inhibitory substances are tested, giving an improved understanding of inhibitor tolerance and effects.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 5, e317-319 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-123670DOI: 10.1016/j.fsigss.2015.09.126OAI: oai:DiVA.org:liu-123670DiVA: diva2:891642
Available from: 2016-01-07 Created: 2016-01-07 Last updated: 2016-01-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Ansell, Ricky
By organisation
BiologyFaculty of Science & Engineering
In the same journal
Forensic Science International: Genetics Supplement Series
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 77 hits
ReferencesLink to record
Permanent link

Direct link