liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Structurally responsive oligonucleotide-based single-probe lateral-flow test for detection of miRNA-21 mimics
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. (Biosensors & Bioelectronics)ORCID iD: 0000-0002-1815-9699
School of Chemistry, Damghan University, Damghan, Iran.
School of Chemistry, Damghan University, Damghan, Iran.
Show others and affiliations
2016 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 408, no 5, 1475-1485 p.Article in journal (Refereed) Published
Abstract [en]

A single-probe strip test for the rapid and sensitive detection of miRNA-21 mimics is reported herein. Highly specific structurally responsive bi-functional, thiol and biotin, DNA/LNA oligonucleotide probes (molecular beacons-MB) were designed and conjugated with gold nanoparticles (AuNPs) (i.e. biotin-MB-AuNPs). The proposed design had the ability to modulate the accessibility of the biotin group as a function of the presence of a miRNA target allowing the interaction of the boilable with the streptavidin test zone only in the presence of the miRNA-21 mimics. For quantitative evaluation, images of the strip tests were recorded using a flatbed scanner (Epson Perfection V370 Photo). The colour intensities of the test zones of the strip tests were analysed with the ImageJ software (Scion Corp., USA) and quantified as a function of pixel intensity. The response of the strip test was linear over the range 0.5 to 20 nM miRNA-21 (limit of detection of 115 pM) and showed good reproducibility (intra and inter CVs below 8 %); furthermore, the assay was shown to be highly selective, discriminating other interference miRNAs mimics (e.g. miRNA-221 and miRNA-205). Finally, the proposed strip test was used for detection of miRNA-21 mimics in spiked serum samples, demonstrating its potential for point-of-care clinical applications. Main advantages of the single-probe strip test design are its versatility, simplicity and robustness, which can be easily extended to other miRNA targets by tuning the sequence of the single probe. Furthermore, the use of the structurally responsive single probe is particularly relevant in the case of short-length targets, such as miRNA, whereas a conventional sandwich approach might require a careful control of assay conditions such as hybridization temperature and salt concentration

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016. Vol. 408, no 5, 1475-1485 p.
Keyword [en]
miRNA-21, molecular beacon (MB), structurally responsive, lateral-flow strip tests
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-123675DOI: 10.1007/s00216-015-9250-9ISI: 000368815200018PubMedID: 26700447OAI: oai:DiVA.org:liu-123675DiVA: diva2:891784
Note

Funding agencies: Ministry of Science, Research and Technology of Iran [214140]

Available from: 2016-01-07 Created: 2016-01-07 Last updated: 2016-02-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Kor, KamalodinTurner, AnthonyBeni, ValerioMak, Wing Cheung
By organisation
Biosensors and BioelectronicsFaculty of Science & Engineering
In the same journal
Analytical and Bioanalytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 484 hits
ReferencesLink to record
Permanent link

Direct link