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Shotgun proteomics to unravel marine mussel (Mytilus edulis) response to long-term exposure to low salinity and propranolol in a Baltic Sea microcosm
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal Lab, environmental proteomics)
Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Stockholm, Sweden. (Cristobal lab, environmental proteomics)
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. (Cristobal Lab, environmental proteomics)
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
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2016 (English)In: Journal of Proteomics, ISSN 1874-3919, E-ISSN 1876-7737, Vol. 137, 97-106 p.Article in journal (Refereed) Published
Abstract [en]

Pharmaceuticals, among them the β-adrenoreceptor blocker propranolol, are an important group of environmental contaminants reported in European waters. Laboratory exposure to pharmaceuticals on marine species has been performed without considering the input of the ecosystem flow. To unravel the ecosystem response to long-term exposure to propranolol we have performed long-term exposure to propranolol and low salinity in microcosms. We applied shotgun proteomic analysis to gills of Mytilus edulis from those Baltic Sea microcosms and identified 2071 proteins with a proteogenomic strategy. The proteome profiling patterns from the 587 highly reproductive proteins among groups define salinity as a key factor in the mussel´s response to propranolol. Exposure at low salinity drives molecular mechanisms of adaptation based on a decrease in the abundance of several cytoskeletal proteins, signalling and intracellular membrane trafficking pathway combined with a response towards the maintenance of transcription and translation. The exposure to propranolol combined with low salinity modulates the expression of structural proteins including cilia functions and decrease the expression membrane protein transporters. This study reinforces the environment concerns of the impact of low salinity in combination with anthropogenic pollutants and anticipate critical physiological conditions for the survival of the blue mussel in the northern areas.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 137, 97-106 p.
Keyword [en]
Mytilus edulis, shotgun proteomics, propranolol, low salinity, environmental monitoring, climate change
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-124213DOI: 10.1016/j.jprot.2016.01.010ISI: 000374368800010OAI: oai:DiVA.org:liu-124213DiVA: diva2:896761
Funder
Swedish Research Council
Note

Funding agencies: Swedish Research Council-Natural Science; VR-NT; Carl Trygger Foundation; Oscar and Lilli Lamms Minne Foundation; Angpanneforening Research Foundation; Magnus Bergsvall Foundation; IKERBASQUE; Basque Foundation for Science; VINNOVA; County Council of Oste

Available from: 2016-01-22 Created: 2016-01-22 Last updated: 2016-06-01Bibliographically approved

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Campos, AlexandreFarinha, Ana PaulaKuruvilla, JacobCristobal, Susana
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