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Insulin-like growth factor I in cats: validation of an enzyme-linked immunosorbent assay and determination of biologic variation
Swedish University of Agriculture Science, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry.
Swedish University of Agriculture Science, Sweden.
Swedish University of Agriculture Science, Sweden.
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2015 (English)In: Veterinary clinical pathology, ISSN 0275-6382, E-ISSN 1939-165X, Vol. 44, no 4, 542-551 p.Article in journal (Refereed) PublishedText
Abstract [en]

Background: Insulin-like growth factor I (IGF-I) measurements are used in veterinary medicine for diagnosing growth hormone disorders. IGF-I assays are subject to interference by IGF-binding proteins (IGFBP) which may not be efficiently removed by standard extraction methods. Adding excess IGF-II during analysis may improve accuracy. Objectives: The purpose of the study was to validate a commercial human IGF-I ELISA which uses excess IGF-II for feline samples and to evaluate biologic variation. Methods: Precision was determined by calculating the coefficient of variation (CV). Accuracy was determined by recovery after removal of IGFBP, addition of IGF-I, and linear dilution after the addition of IGFBP. Biologic variation was determined by repeated sampling in 7 cats. Results: There was interference by IGFBP in the high measuring range, resulting in falsely low IGF-I concentrations. This was overcome by the addition of high concentrations of IGF-II. Untreated serum had a measured/expected ratio of 98-115% compared to serum where IGFBP had been removed. Recovery after the addition of IGF-I was 83-112%. Inter and intra-assay CVs ranged from 2.4% to 5.0% which is within the minimum acceptance criteria based on biologic variation. The reference interval of IGF-I was wide (90-1207 ng/mL) and there was a significant association between body weight and ln IGF-I (P < .000001). Conclusions: This human ELISA is suitable for feline samples, but interfering IGFBP can cause falsely low concentrations. It is recommended to dilute samples such that IGF-I is < 28 ng/mL on the standard curve to grant for sufficient IGF-II for binding of interferent IGFBP.

Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2015. Vol. 44, no 4, 542-551 p.
Keyword [en]
Growth hormone disorders; insulin-like growth factor-binding proteins; size-exclusion chromatography
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-125173DOI: 10.1111/vcp.12289ISI: 000368418900012PubMedID: 26418310OAI: oai:DiVA.org:liu-125173DiVA: diva2:903368
Note

Funding Agencies|Agria and Swedish Kennel Club Research Foundation; Michael Forsgren Foundation

Available from: 2016-02-15 Created: 2016-02-15 Last updated: 2016-02-15

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Theodorsson, Elvar
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Division of Microbiology and Molecular MedicineFaculty of Medicine and Health SciencesDepartment of Clinical Chemistry
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