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Structural Insights into the Calcium-Mediated Allosteric Transition in the C-Terminal Domain of Calmodulin from Nuclear Magnetic Resonance Measurements
University of Cambridge, England.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.
University of Cambridge, England.
Red Glead Discovery, Sweden.
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2016 (English)In: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 55, no 1, 19-28 p.Article in journal (Refereed) PublishedText
Abstract [en]

Calmodulin is a two-domain signaling protein that becomes activated upon binding cooperatively two pairs of calcium ions, leading to large-scale conformational changes that expose its binding site. Despite significant advances in understanding the structural biology of calmodulin functions, the mechanistic details of the conformational transition between closed and open states have remained unclear. To investigate this transition, we used a combination of molecular dynamics simulations and nuclear magnetic resonance (NMR) experiments on the Ca2+-saturated E140Q C-terminal domain variant. Using chemical shift restraints in replica-averaged metadynamics simulations, we obtained a high-resolution structural ensemble consisting of two conformational states and validated such an ensemble against three independent experimental data sets, namely, interproton nuclear Overhauser enhancements, N-15 order parameters, and chemical shift differences between the exchanging states. Through a detailed analysis of this structural ensemble and of the corresponding statistical weights, we characterized a calcium-mediated conformational transition whereby the coordination of Ca2+ by just one oxygen of the bidentate ligand E140 triggers a concerted movement of the two EF-hands that exposes the target binding site. This analysis provides atomistic insights into a possible Ca2+-mediated activation mechanism of calmodulin that cannot be achieved from static structures alone or from ensemble NMR measurements of the transition between conformations.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2016. Vol. 55, no 1, 19-28 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-125154DOI: 10.1021/acs.biochem.5b00961ISI: 000368323000003PubMedID: 26618792OAI: oai:DiVA.org:liu-125154DiVA: diva2:903418
Note

Funding Agencies|Swedish Research Council [Dnr 2012-5136, Dnr 2010-4912]; Goran Gustafsson Foundation for Research in Natural Sciences and Medicine; Knut and Alice Wallenberg Foundation; Biotechnology and Biological Sciences Research Council [BB/H013318/1]; Federation of European Biochemical Societies Fellowship; Marie Curie Intra European Fellowship

Available from: 2016-02-15 Created: 2016-02-15 Last updated: 2016-02-15

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ReferencesLink to record
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