Electrocatalytic Currents from Single Enzyme Molecules
2016 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 138, no 8, 2504-2507 p.Article in journal (Refereed) Published
Single molecule enzymology provides an opportunity to examine details of enzyme mechanisms that are not distinguishable in biomolecule ensemble studies. Here we report, for the first time, detection of the current produced in an electrocatalytic reaction by a single redox enzyme molecule when it collides with an ultramicroelectrode. The catalytic process provides amplification of the current from electron-transfer events at the catalyst leading to a measurable current. This new methodology monitors turnover of a single enzyme molecule. The methodology might complement existing single molecule techniques, giving further insights into enzymatic mechanisms and filling the gap between fundamental understanding of biocatalytic processes and their potential for bioenergy production.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2016. Vol. 138, no 8, 2504-2507 p.
Chemical Sciences Chemical Engineering Chemical Process Engineering
IdentifiersURN: urn:nbn:se:liu:diva-125241DOI: 10.1021/Jacs.5b13149ISI: 000371453700011OAI: oai:DiVA.org:liu-125241DiVA: diva2:903864
Funding agencies: Swedish research council Formas [245-2010-1062]; research center Security Link (VINNOVA ) [2009-00966]; Centre in Nano Science and Technology (CeNano, Linkoping University)
Vid tiden för dispuation förelåg publikationen endast som manuskript2016-02-172016-02-172016-04-07Bibliographically approved