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Immunogold electron microscopic quantification of small molecular compounds and proteins at synapses and other neural profiles
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0003-3584-7829
Department of Oral Biology, University of Oslo, Norway.
Department of Anatomy, Institute for Basic Medical Sciences, University of Oslo, Norway.
2015 (English)In: Immunocytochemistry and related techniques: Part IV / [ed] Adalberto Merighi and Laura Lossi, Springer-Verlag New York, 2015, 281-297 p.Chapter in book (Other academic)
Abstract [en]

This chapter describes procedures for quantifi cation of postembedding labeling at brain synapses using computer-based tools. The postembedding electron microscopic immunogold method allows detection of epitopes with a resolution of about 20–30 nm. However, plasma membranes belonging to different cells and membranes of intracellular organelles can often be located even closer together. Localizing epitopes at such membranes can reliably be performed by using computer programs, such as ImageJ, which offers automated quantifi cation of gold particles. The present chapter provides a practical description of how to use ImageJ and plug- ins to obtain an accurate representation of the subcellular localization of proteins and small molecular compounds.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. 281-297 p.
Series
, Neuromethods, ISSN 0893-2336 ; 101
Keyword [en]
astrocyte, ImageJ, spine, synapse, terminal
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:liu:diva-125404DOI: 10.1007/978-1-4939-2313-7_15ISBN: 978-1-4939-2312-0 (Print)ISBN: 978-1-4939-2313-7 (Online)OAI: oai:DiVA.org:liu-125404DiVA: diva2:905467
Available from: 2016-02-22 Created: 2016-02-22 Last updated: 2016-03-02

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