Strong Maternal Effects on Gene Expression inArabidopsis lyrata Hybrids
2016 (English)In: Molecular Biology and Evolution, ISSN 0737-4038, Vol. 33, no 4, 984-994 p.Article in journal (Refereed) Published
Hybridization between populations or species can have pronounced fitness consequences. Yet little is known about howhybridization affects gene regulation. Three main models have been put forward to explain gene expression patterns inhybrids: additive, dominance, or parental effects. Here, we use high throughput RNA-sequencing to examine the extent towhich hybrid gene expression follows predictions by each of the three models. We performed a reciprocal crossingexperiment between two differentiated populations of the perennial herb Arabidopsis lyrata and sequenced RNA inrosette leaves of 12-week-old plants grown in greenhouse conditions. The two parental populations had highly differentiatedgene expression patterns. In hybrids, a majority of genes showed intermediate expression relative to that of theirparental populations (i.e., additive effects), but expression was frequently more similar to the maternal than to theirpaternal population (i.e., maternal effects). Allele-specific expression analyses showed that in the vast majority of cases,genes with pronounced maternal effect expressed both the maternal and the paternal allele. Maternal effects on hybridgene expression have rarely been documented previously and our study suggests it could be more common thanpreviously assumed. Whether the maternal effect on gene expression persists to later life-stages, and whether thevariation in gene expression is manifested in other aspects of the phenotype, remain to be elucidated. Our findingsare relevant for understanding the consequences of outbreeding and hybridization and open up several questions forfuture studies.
Place, publisher, year, edition, pages
Oxford University Press, 2016. Vol. 33, no 4, 984-994 p.
hybridization, gene expression, maternal effect, additive effect, Arabidopsis lyrata, RNA-Seq
IdentifiersURN: urn:nbn:se:liu:diva-125904DOI: 10.1093/molbev/msv342ISI: 000374226700011PubMedID: 26685177OAI: oai:DiVA.org:liu-125904DiVA: diva2:909598