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Detection of Matrilysin Activity Using Polypeptide Functionalized Reduced Graphene Oxide Field-Effect Transistor Sensor
Nanyang Technology University, Singapore; Nanyang Technology University, Singapore; University of Loughborough, England.
Nanyang Technology University, Singapore; Centre Biomimet Sensor Science, Singapore.
Nanyang Technology University, Singapore; Nanyang Technology University, Singapore.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
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2016 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 88, no 6, 2994-2998 p.Article in journal (Refereed) PublishedText
Abstract [en]

A novel approach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradation of various macromolecules) based on a polypeptide (JR2EC) functionalized reduced graphene oxide (rGO) field effect transistor (FET) is reported. MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, thereby modulating the conductance of rGO-FET. The proposed assay enabled detection of MMP-7 at clinically relevant concentrations with a limit of detection (LOD) of 10 ng/mL (400 pM), attributed to the significant reduction of the net charge of JR2EC upon digestion by MMP-7. Quantitative detection of MMP-7 in human plasma was further demonstrated with a LOD of 40 ng/mL, illustrating the potential for the proposed methodology for tumor detection and carcinoma diagnostic (e.g., lung cancer and salivary gland cancer). Additionally, excellent specificity of the proposed assay was demonstrated using matrix metallopeptidase 1 (MMP-1), a protease of the same family. With appropriate selection and modification of polypeptides, the proposed assay could be extended for detection of other enzymes with polypeptide digestion capability.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2016. Vol. 88, no 6, 2994-2998 p.
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Physical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-127272DOI: 10.1021/acs.analchem.5b04663ISI: 000372391500003PubMedID: 26887256OAI: oai:DiVA.org:liu-127272DiVA: diva2:921608
Note

Funding Agencies|Institute for Sports Research, Nanyang Technological University; MOE-Tier 1 [2014-T1-001-133-01]

Available from: 2016-04-20 Created: 2016-04-19 Last updated: 2016-04-20

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Selegård, RobertAili, Daniel
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ReferencesLink to record
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