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Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Mazandaran, Iran.
University of Mazandaran, Iran.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, 566-573 p.Article in journal (Refereed) Published
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Text
Abstract [en]

Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of food borne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs. We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1 x 10(1) to 1 x 10(8) CFU mL(-1), with a limit of quantification (LOQ) of 1 x 10(1) CFU mL(-1) and a limit of detection (LOD) of 6 CFU mL(-1). Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1 x 10(2), 1 x 10(4) and 1 x 10(6) CFU mL(-1)) apple juice samples. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2016. Vol. 80, 566-573 p.
Keyword [en]
Diazonium grafting; Aptamer; S. typhimurium; Label-free detection; Electrochemical impedance spectroscopy; Food analysis
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-127249DOI: 10.1016/j.bios.2016.02.024ISI: 000372558500079PubMedID: 26894987OAI: oai:DiVA.org:liu-127249DiVA: diva2:921644
Note

Funding Agencies|Vetenskapsradet (Pathoscreen project; Swedish Research Link) [D0675001]; Ministry of Science Research and Technology of Iran

Available from: 2016-04-20 Created: 2016-04-19 Last updated: 2016-06-29Bibliographically approved

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