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Label-free impedimetric biosensor for Salmonella Typhimurium detection based on poly [pyrrole-co-3-carboxyl-pyrrole] copolymer supported aptamer
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. S University of Mashhad, Iran.
Ferdowsi University of Mashhad, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-1815-9699
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-2071-7768
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, 194-200 p.Article in journal (Refereed) PublishedText
Abstract [en]

The Gram-negative bacterium, Salmonella Typhimurium (S. Typhimurium) is a food borne pathogen responsible for numerous hospitalisations and deaths all over the world. Conventional detection methods for pathogens are time consuming and labour-intensive. Hence, there is considerable interest in faster and simpler detection methods. Polypyrrole-based polymers, due to their intrinsic chemical and electrical properties, have been demonstrated to be valuable candidates for the fabrication of chemo/biosensors and functional surfaces. Similarly aptamers have been shown to be good alternatives to antibodies in the development of affinity biosensors. In this study, we report on the combination of Poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and aptamer for the development of a label-less electrochemical biosensor suitable for the detection of S. Typhimurium. Impedimetric measurements were facilitated by the effect of the aptamer/target interaction on the intrinsic conjugation of the poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and subsequently on its electrical properties. The aptasensor detected S. Typhimurium in the concentration range 10(2)-10(8) CFU mL(-1) with high selectivity over other model pathogens and with a limit of quantification (LOQ) of 100 CFU mL(-1) and a limit of detection (LOD) of 3 CFU mL(-1). The suitability of the aptasensor for real sample detection was demonstrated via recovery studies performed in spiked apple juice samples. We envisage this to be a viable approach for the inexpensive and rapid detection of pathogens in food, and possibly in other environmental samples. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2016. Vol. 80, 194-200 p.
Keyword [en]
S. Typhimurium; Aptamer; Electrochemical impedance spectroscopy; Poly [pyrrole-co-3-carboxyl-pyrrole]; Label-free detection
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-127248DOI: 10.1016/j.bios.2016.01.057ISI: 000372558500029PubMedID: 26836649OAI: oai:DiVA.org:liu-127248DiVA: diva2:922157
Note

Funding Agencies|Ministry of Science Research and Technology of Iran; Research Council of Ferdowsi University of Mashhad [3/29828]; Linkoping University, Vetenskapsradet [D0675001]; Swedish Research Council [VR-2014-3079]

Available from: 2016-04-22 Created: 2016-04-19 Last updated: 2016-06-17

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