liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA
Vet Teaching Hospital, Spain.
Vet Teaching Hospital, Spain.
Swedish University of Agriculture Science, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-5194-2124
Show others and affiliations
2016 (English)In: Reproduction in domestic animals (1990), ISSN 0936-6768, E-ISSN 1439-0531, Vol. 51, no 1, 18-25 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling of spermatozoa - including sex sorting - as basic ground for oxidative damage. The aim of this study was to disclose whether the flow cytometric sorting procedure increases the production of reactive oxygen species (ROS), and to identify if ROS production relates to DNA damage in sorted spermatozoa using specific flow cytometry-based assays. After sorting, oxidative stress increased from 26% to 33% in pre-and post-incubation controls, to 46% after sex sorting (p < 0.05). Proportions of DNA fragmentation index post-sorting were approximately 10% higher (31.3%); an effect apparently conduced via oxidative DNA damage as revealed by the oxyDNA assay. The probable origin of this increased oxidative stress owes the removal of enough seminal plasma due to the unphysiological sperm extension, alongside a deleterious effect of high pressure on mitochondria during the sorting procedure.

Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2016. Vol. 51, no 1, 18-25 p.
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-127456DOI: 10.1111/rda.12640ISI: 000373107600003PubMedID: 26592367OAI: oai:DiVA.org:liu-127456DiVA: diva2:925206
Note

Funding Agencies|Ministerio de Economia y Competitividad-FEDER in Madrid, Spain [AGL2013-43211-R]; Gobierno de Extremadura-FEDER [GR 10010, PCE1002]

Available from: 2016-04-30 Created: 2016-04-26 Last updated: 2016-05-17

Open Access in DiVA

fulltext(252 kB)45 downloads
File information
File name FULLTEXT01.pdfFile size 252 kBChecksum SHA-512
73f22993a55124b6a5ba91eff03de477982afc16510bb59054ea90147da684bf1c94336f3e98ecabc9df4ec3b0fa773c7f8904b3670c9099b6c9608d8142214e
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
By organisation
Division of Clinical SciencesFaculty of Medicine and Health Sciences
In the same journal
Reproduction in domestic animals (1990)
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 45 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 161 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf