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Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA
Vet Teaching Hospital, Spain.
Vet Teaching Hospital, Spain.
Swedish University of Agriculture Science, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-5194-2124
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2016 (English)In: Reproduction in domestic animals (1990), ISSN 0936-6768, E-ISSN 1439-0531, Vol. 51, no 1, 18-25 p.Article in journal (Refereed) PublishedText
Abstract [en]

To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling of spermatozoa - including sex sorting - as basic ground for oxidative damage. The aim of this study was to disclose whether the flow cytometric sorting procedure increases the production of reactive oxygen species (ROS), and to identify if ROS production relates to DNA damage in sorted spermatozoa using specific flow cytometry-based assays. After sorting, oxidative stress increased from 26% to 33% in pre-and post-incubation controls, to 46% after sex sorting (p < 0.05). Proportions of DNA fragmentation index post-sorting were approximately 10% higher (31.3%); an effect apparently conduced via oxidative DNA damage as revealed by the oxyDNA assay. The probable origin of this increased oxidative stress owes the removal of enough seminal plasma due to the unphysiological sperm extension, alongside a deleterious effect of high pressure on mitochondria during the sorting procedure.

Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2016. Vol. 51, no 1, 18-25 p.
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:liu:diva-127456DOI: 10.1111/rda.12640ISI: 000373107600003PubMedID: 26592367OAI: oai:DiVA.org:liu-127456DiVA: diva2:925206
Note

Funding Agencies|Ministerio de Economia y Competitividad-FEDER in Madrid, Spain [AGL2013-43211-R]; Gobierno de Extremadura-FEDER [GR 10010, PCE1002]

Available from: 2016-04-30 Created: 2016-04-26 Last updated: 2016-05-17

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Rodriguez-Martinez, Heriberto
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