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Detection limits of 405 nm and 633 nm excited PpIX fluorescence for brain tumor detection during stereotactic biopsy
Klinikum der Univ. München.
MRC Systems GmbH.
Linköping University, The Institute of Technology. Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. (MINT)ORCID iD: 0000-0002-0555-8877
Klinikum der Univ. München.
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2016 (English)In: Biophotonics: Photonic Solutions for Better Health Care V,   03 April , 2016 ,Brussels, Belgium / [ed] Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco S. Pavone, Brussels, 2016, Vol. 9887, 98872Z-1-98872Z-12 p.Conference paper (Refereed)
Abstract [en]

5-aminolevulinic-acid-(5-ALA)-induced protoporphyrin IX (PpIX) fluorescence may be used to improve stereotactic brain tumor biopsies. In this study, the sensitivity of PpIX-based tumor detection has been investigated for two potential excitation wavelengths (405 nm, 633 nm). Using a 200 μm fiber in contact with semi-infinite optical phantoms containing ink and Lipovenös, PpIX detection limits of 4.0 nM and 200 nM (relating to 1 mW excitation power) were determined for 405 nm and 633 nm excitation, respectively. Hence, typical PpIX concentrations in glioblastomas of a few μM should be well detectable with both wavelengths. Additionally, blood layers of selected thicknesses were placed between fiber and phantom. Red excitation was shown to be considerably less affected by blood interference: A 50 μm blood layer, for instance, blocked the 405- nm-excited fluorescence completely, but reduced the 633-nm-excited signal by less than 50%. Ray tracing simulations demonstrated that – without blood layer – the sensitivity advantage of 405 nm rises for decreasing fluorescent volume from 50-fold to a maximum of 100-fold. However, at a tumor volume of 1 mm3, which is a typical biopsy sample size, the 633-nm-excited fluorescence signal is only reduced by about 10%. Further simulations revealed that with increasing fiber-tumor distance, the signal drops faster for 405 nm. This reduces the risk of detecting tumor tissue outside the needle’s coverage, but diminishes the overlap between optically and mechanically sampled volumes. While 405 nm generally offers a higher sensitivity, 633 nm is more sensitive to distant tumors and considerably superior in case of blood-covered tumor tissue. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Place, publisher, year, edition, pages
Brussels, 2016. Vol. 9887, 98872Z-1-98872Z-12 p.
, Proceedings of SPIE, ISSN 1996756X
Keyword [en]
Fluorescence, stereotactic biopsy, brain tumor, PpIX
National Category
Computational Mathematics
URN: urn:nbn:se:liu:diva-127671DOI: 10.1117/12.2225234OAI: diva2:926375
Photonics Europe, SPIE
Available from: 2016-05-06 Created: 2016-05-06 Last updated: 2016-05-12

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Haj-Hosseini, Neda
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The Institute of TechnologyBiomedical Instrumentation
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