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Endogenous Sulfur Dioxide Inhibits Vascular Calcification in Association with the TGF-beta/Smad Signaling Pathway
Peking University, Peoples R China.
Peking University, Peoples R China.
Peking University, Peoples R China; Minist Educ, Peoples R China.
Linköping University, Department of Medical and Health Sciences. Linköping University, Faculty of Medicine and Health Sciences.
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2016 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 17, no 3Article in journal (Refereed) PublishedText
Abstract [en]

The study was designed to investigate whether endogenous sulfur dioxide (SO2) plays a role in vascular calcification (VC) in rats and its possible mechanisms. In vivo medial vascular calcification was induced in rats by vitamin D3 and nicotine for four weeks. In vitro calcification of cultured A7r5 vascular smooth muscle cells (VSMCs) was induced by calcifying media containing 5 mmol/L CaCl2. Aortic smooth muscle (SM) alpha-actin, runt-related transcription factor 2 (Runx2), transforming growth factor-beta (TGF-beta) and Smad expression was measured. VC rats showed dispersed calcified nodules among the elastic fibers in calcified aorta with increased aortic calcium content and alkaline phosphatase (ALP) activity. SM alpha-actin was markedly decreased, but the osteochondrogenic marker Runx2 concomitantly increased and TGF-beta/Smad signaling was activated, in association with the downregulated SO2/aspartate aminotransferase (AAT) pathway. However, SO2 supplementation successfully ameliorated vascular calcification, and increased SM alpha-actin expression, but inhibited Runx2 and TGF-beta/Smad expression. In calcified A7r5 VSMCs, the endogenous SO2/AAT pathway was significantly downregulated. SO2 treatment reduced the calcium deposits, calcium content, ALP activity and Runx2 expression and downregulated the TGF-beta/Smad pathway in A7r5 cells but increased SM alpha-actin expression. In brief, SO2 significantly ameliorated vascular calcification in association with downregulation of the TGF-beta/Smad pathway.

Place, publisher, year, edition, pages
MDPI AG , 2016. Vol. 17, no 3
Keyword [en]
vascular calcification; sulfur dioxide; smooth muscle cell; transforming growth factor-beta
National Category
Basic Medicine
URN: urn:nbn:se:liu:diva-127752DOI: 10.3390/ijms17030266ISI: 000373712800147PubMedID: 26907267OAI: diva2:927502

Funding Agencies|National Natural Science Foundation of China [81400311, 31130030]; Major Basic Research Development Program of China [2012CB517806, 2013CB933801]

Available from: 2016-05-12 Created: 2016-05-12 Last updated: 2016-06-01

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Dong Liu, Angie
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