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Lab on chip microdevices for cellular mechanotransduction in urothelial cells
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
Karolinska Institute, Sweden.
Karolinska Institute, Sweden.
Karolinska Institute, Sweden.
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2016 (English)In: Proc. SPIE 9798, Electroactive Polymer Actuators and Devices (EAPAD) 2016, SPIE - International Society for Optical Engineering, 2016, Vol. 9798, 97981R-1-97981R-9 p.Conference paper, Published paper (Refereed)
Abstract [en]

Cellular mechanotransduction is crucial for physiological function in the lower urinary tract. The bladder is highly dependent on the ability to sense and process mechanical inputs, illustrated by the regulated filling and voiding of the bladder. However, the mechanisms by which the bladder integrates mechanical inputs, such as intravesicular pressure, and controls the smooth muscles, remain unknown. To date no tools exist that satisfactorily mimic in vitro the dynamic micromechanical events initiated e.g. by an emerging inflammatory process or a growing tumour mass in the urinary tract. More specifically, there is a need for tools to study these events on a single cell level or in a small population of cells. We have developed a micromechanical stimulation chip that can apply physiologically relevant mechanical stimuli to single cells to study mechanosensitive cells in the urinary tract. The chips comprise arrays of microactuators based on the electroactive polymer polypyrrole (PPy). PPy offers unique possibilities and is a good candidate to provide such physiological mechanical stimulation, since it is driven at low voltages, is biocompatible, and can be microfabricated. The PPy microactuators can provide mechanical stimulation at different strains and/or strain rates to single cells or clusters of cells, including controls, all integrated on one single chip, without the need to preprepare the cells. This paper reports initial results on the mechano-response of urothelial cells using the micromechanical stimulation chips. We show that urothelial cells are viable on our microdevices and do respond with intracellular Ca2+ increase when subjected to a micro-mechanical stimulation.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2016. Vol. 9798, 97981R-1-97981R-9 p.
Series
SPIE proceedings, ISSN 0277-786X
National Category
Medical Engineering
Identifiers
URN: urn:nbn:se:liu:diva-128248DOI: 10.1117/12.2218799ISI: 000388439700034ISBN: 978-1-5106-0039-3 (print)OAI: oai:DiVA.org:liu-128248DiVA: diva2:930337
Conference
SPIE Electroactive Polymer Actuators and Devices (EAPAD) 2016
Funder
Swedish Research Council, VR-2014-3079
Note

Funding agencies: Swedish Research Council [VR-2014-3079]; Linkoping University; European Science Foundation COST Action ESNAM (European Scientific Network for Artificial Muscles) [MP1003]; Swedish Society of Medicine [SLS-249841]

Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2017-02-24

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Publisher's full texthttp://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2516017

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