Whether preference for subject-extracted relative clauses in language processing is a universal rule has been debated with evidences from both the first and the second language acquisition studies. But very few studies focus on learners of Chinese as a second language. The current research studied Chinese subject/object-extracted relative clauses processing among learners of Chinese as a second language by the self-paced reading experiment. The results demonstrate a faster and more accurate processing of subject-extracted relative clauses in both subject and object modifying conditions, adding more evidence to the universal preference for the subject-extracted relative clauses. Both Frequency-based Accounts and Memory-based Accounts are discussed related to the current findings.