liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
EPH/ephrin profile and EPHB2 expression predicts patient survival in breast cancer
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. St Anna Kinderkrebsforsch eV, Austria.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Bioinformatics. Linköping University, Faculty of Science & Engineering.
Karolinska University Hospital, Sweden; Karolinska Institute, Sweden.
Show others and affiliations
2016 (English)In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 7, no 16, 21362-21380 p.Article in journal (Refereed) PublishedText
Abstract [en]

The EPH and ephrins function as both receptor and ligands and the output on their complex signaling is currently investigated in cancer. Previous work shows that some EPH family members have clinical value in breast cancer, suggesting that this family could be a source of novel clinical targets. Here we quantified the mRNA expression levels of EPH receptors and their ligands, ephrins, in 65 node positive breast cancer samples by RT-PCR with TaqMan (R) Micro Fluidics Cards Microarray. Upon hierarchical clustering of the mRNA expression levels, we identified a subgroup of patients with high expression, and poor clinical outcome. EPHA2, EPHA4, EFNB1, EFNB2, EPHB2 and EPHB6 were significantly correlated with the cluster groups and particularly EPHB2 was an independent prognostic factor in multivariate analysis and in four public databases. The EPHB2 protein expression was also analyzed by immunohistochemistry in paraffin embedded material (cohort 2). EPHB2 was detected in the membrane and cytoplasmic cell compartments and there was an inverse correlation between membranous and cytoplasmic EPHB2. Membranous EPHB2 predicted longer breast cancer survival in both univariate and multivariate analysis while cytoplasmic EPHB2 indicated shorter breast cancer survival in univariate analysis. Concluding: the EPH/EFN cluster analysis revealed that high EPH/EFN mRNA expression is an independent prognostic factor for poor survival. Especially EPHB2 predicted poor breast cancer survival in several materials and EPHB2 protein expression has also prognostic value depending on cell localization.

Place, publisher, year, edition, pages
IMPACT JOURNALS LLC , 2016. Vol. 7, no 16, 21362-21380 p.
Keyword [en]
EPHB2; EPH family; TaqMan array; gene expression; protein expression
National Category
Cancer and Oncology
URN: urn:nbn:se:liu:diva-130143DOI: 10.18632/oncotarget.7246ISI: 000377705900023PubMedID: 26870995OAI: diva2:948555

Funding Agencies|Swedish Research Council; Stockholm Cancer Society; Oncology Clinics Research Fund of Linkoping

Available from: 2016-07-12 Created: 2016-07-11 Last updated: 2016-08-05

Open Access in DiVA

fulltext(3648 kB)11 downloads
File information
File name FULLTEXT01.pdfFile size 3648 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Husa, Anna-MariaMagic, ZeljanaLarsson, MalinPerez-Tenorio, Gizeh
By organisation
Division of Clinical SciencesFaculty of Medicine and Health SciencesBioinformaticsFaculty of Science & Engineering
In the same journal
Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar
Total: 11 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link