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Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa.
ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León, Spain.
ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León, Spain.
Cabárceno Park, Cantabria, Spain.
ITRA-ULE, INDEGSAL, University of León, León, Spain; Cell Biology, University of León, León, Spain.
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2012 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 77, no 6, 1119-1128 p.Article in journal (Refereed) Published
Abstract [en]

Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 77, no 6, 1119-1128 p.
Keyword [en]
Centrifugation, Density gradient, Cryopreservation, Brown bear, Spermatozoa
National Category
Developmental Biology
Identifiers
URN: urn:nbn:se:liu:diva-130193DOI: 10.1016/j.theriogenology.2011.10.016PubMedID: 22154477OAI: oai:DiVA.org:liu-130193DiVA: diva2:948915
Available from: 2016-07-14 Created: 2016-07-14 Last updated: 2016-07-25Bibliographically approved

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Alvarez-Rodriguez, Manuel
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