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Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-0256-1958
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0003-0120-354X
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-5194-2124
2016 (English)In: Reproduction in domestic animals (1990), ISSN 0936-6768, E-ISSN 1439-0531Article in journal (Refereed) Epub ahead of print
Abstract [en]

Freezing of boar spermatozoa includes the cryoprotectant glycerol, but renders low cryosurvival, owing to major changes in osmolarity during freezing/thawing. We hypothesize that aquaporins (AQPs) 7 and 9 adapt their membrane domain location to these osmotic challenges, thus maintaining sperm homeostasis. Western blotting (WB) and immunocytochemistry (ICC) at light and electron microscope levels with several commercial primary antibodies and protocols explored AQP location on cauda epididymal and ejaculated spermatozoa (from different fractions of the ejaculate), unprocessed, extended, chilled and frozen-thawed. Although differences in WB and ICC labelling were seen among antibodies, AQP-7 was conspicuously located in the entire tail and cytoplasmic droplet in caudal spermatozoa, being restricted to the mid-piece and principal piece domains in ejaculated spermatozoa. AQP-9 was mainly localized in the sperm head in both caudal and ejaculated spermatozoa. While unaffected by chilling (+5°C), freezing and thawing of ejaculated spermatozoa clearly relocated the head labelling of AQP-7, but not that of AQP-9. In vitro mimicking of cell membrane expansion during quick thawing maintained the localization of AQP-9 but relocated AQP-7 towards the acrosome. AQP-7, but not AQP-9, appears as a relevant marker for non-empirical studies of sperm handling.

Place, publisher, year, edition, pages
Blackwell Verlag, 2016.
National Category
Developmental Biology
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URN: urn:nbn:se:liu:diva-130228DOI: 10.1111/rda.12728PubMedID: 27405395OAI: oai:DiVA.org:liu-130228DiVA: diva2:949479
Available from: 2016-07-19 Created: 2016-07-19 Last updated: 2016-08-21Bibliographically approved

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The full text will be freely available from 2017-07-13 18:21
Available from 2017-07-13 18:21

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Vicente-Carrillo, AlejandroÁlvarez-Rodríguez, ManuelRodriguez-Martinez, Heriberto
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Division of Clinical SciencesFaculty of Medicine and Health Sciences
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Reproduction in domestic animals (1990)
Developmental Biology

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