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Rye B chromosomes encode a functional Argonaute-like proteinwith in vitro slicer activities similar to its A chromosome paralog
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, 06466 Stadt Seeland, Germany;.
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, 06466 Stadt Seeland, Germany;.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Institute of Bioinformatics and Systems Biology/Munich Information Center for Protein Sequences, Helmholtz Center Munich, German Research Center for Environmental Health, 85764 Neuherberg, Germany.
Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Faculty of Life Sciences, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, 06120 Halle/Saale, Germany.
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2017 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 213, no 2, 904-916 p.Article in journal (Refereed) Published
Abstract [en]
  • B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, whichappear in many different species of eukaryote. So far, Bs have been considered to be geneti-cally inert elements without any functional genes.
  • Our comparative transcriptome analysis and the detection of active RNA polymerase II(RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) con-tribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open read-ing frame were expressed in generative and vegetative tissues, respectively. In addition to B-derived transposable element transcripts, a high percentage of short transcripts withoutdetectable similarity to known proteins and gene fragments from A chromosomes (As) werefound, suggesting an ongoing gene erosion process.
  • In vitro analysis of the A- and B-encoded AGO4B protein variants demonstrated that bothpossess RNA slicer activity. These data demonstrate unambiguously the presence of a func-tional AGO4B gene on Bs and that these Bs carry both functional protein coding genes andpseudogene copies.
  • Thus, B-encoded genes may provide an additional level of gene control and complexity incombination with their related A-located genes. Hence, physiological effects, associated withthe presence of Bs, may partly be explained by the activity of B-located (pseudo)genes.
Place, publisher, year, edition, pages
Wiley-Blackwell, 2017. Vol. 213, no 2, 904-916 p.
Keyword [en]
Argonaute, B chromosomes, B-located genes, gene erosion. gene expression, pseudogenization, Secale cereale
National Category
Genetics
Identifiers
URN: urn:nbn:se:liu:diva-130342DOI: 10.1111/nph.14110ISI: 000391452300039PubMedID: 27468091OAI: oai:DiVA.org:liu-130342DiVA: diva2:950301
Note

Funding agencies: China CSC scholarship; DFG Germany [HO 1779/10-1/14-1, CRC 648 C1, A7]; National Program of Sustainability [LO 2014]; Czech Science Foundation [P501-12-G090]

Available from: 2016-07-29 Created: 2016-07-29 Last updated: 2017-02-06

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