Rye B chromosomes encode a functional Argonaute-like proteinwith in vitro slicer activities similar to its A chromosome paralog
2016 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137Article in journal (Refereed) Epub ahead of print
- B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, whichappear in many different species of eukaryote. So far, Bs have been considered to be geneti-cally inert elements without any functional genes.
- Our comparative transcriptome analysis and the detection of active RNA polymerase II(RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) con-tribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open read-ing frame were expressed in generative and vegetative tissues, respectively. In addition to B-derived transposable element transcripts, a high percentage of short transcripts withoutdetectable similarity to known proteins and gene fragments from A chromosomes (As) werefound, suggesting an ongoing gene erosion process.
- In vitro analysis of the A- and B-encoded AGO4B protein variants demonstrated that bothpossess RNA slicer activity. These data demonstrate unambiguously the presence of a func-tional AGO4B gene on Bs and that these Bs carry both functional protein coding genes andpseudogene copies.
- Thus, B-encoded genes may provide an additional level of gene control and complexity incombination with their related A-located genes. Hence, physiological effects, associated withthe presence of Bs, may partly be explained by the activity of B-located (pseudo)genes.
Place, publisher, year, edition, pages
Argonaute, B chromosomes, B-located genes, gene erosion. gene expression, pseudogenization, Secale cereale
IdentifiersURN: urn:nbn:se:liu:diva-130342DOI: 10.1111/nph.14110PubMedID: 27468091OAI: oai:DiVA.org:liu-130342DiVA: diva2:950301