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Treatment of platelet concentrates with ultraviolet C light for pathogen reduction increases cytokine accumulation
Karolinska University Hospital, Sweden; Karolinska Institute, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Immunology and Transfusion Medicine.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Region Östergötland, Center for Diagnostics, Department of Clinical Immunology and Transfusion Medicine. Linköping University, Faculty of Medicine and Health Sciences.
2016 (English)In: Transfusion, ISSN 0041-1132, E-ISSN 1537-2995, Vol. 56, no 6, 1377-1383 p.Article in journal (Refereed) PublishedText
Abstract [en]

BACKGROUNDPathogen reduction technologies use photoactive substances in combination with ultraviolet (UV) light to inactivate pathogens. A new method uses only UVC light for pathogen reduction. This study assesses the effects of UVC light treatment on cytokine release in platelet (PLT) concentrates (PCs). STUDY DESIGN AND METHODSA PC with 35% plasma and 65% PLT additive solution (SSP+) was prepared from five buffy coats. Three such PCs were pooled and divided into 3 units. One unit was used as a nonirradiated control, the second was a gamma-irradiated control, and the third unit was treated with UVC light technology. Ten units of each type were investigated. Cytokine release was analyzed on Days 1, 5, and 7 of storage. Correlation between cytokines, PLT surface markers, and hemostatic properties was investigated. RESULTSSwirling was well preserved and pH was above the reference limit of 6.4 during storage of PLTs in all groups. Cytokine levels increased during storage in all groups but to a larger degree in PCs treated with UVC light. Only weak correlation was found between cytokines and PLT surface markers (ramp;lt;0.5). However, several cytokines showed strong correlation (ramp;gt;0.6) with the PLTs ability to promote clot retraction. CONCLUSIONUVC treatment resulted in increased release from PLT alpha granules as evident by a higher cytokine release compared to nonirradiated and gamma-irradiated PCs. The clinical relevance of these findings needs to be further evaluated.

Place, publisher, year, edition, pages
WILEY-BLACKWELL , 2016. Vol. 56, no 6, 1377-1383 p.
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:liu:diva-130296DOI: 10.1111/trf.13601ISI: 000378553200048PubMedID: 27080102OAI: oai:DiVA.org:liu-130296DiVA: diva2:950502
Note

Funding Agencies|Region Ostergotland; Linkoping Medical Society; MacoPharma

Available from: 2016-07-31 Created: 2016-07-28 Last updated: 2016-07-31

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Berlin, GöstaTynngård, Nahreen
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Division of Neuro and Inflammation ScienceFaculty of Medicine and Health SciencesDepartment of Clinical Immunology and Transfusion MedicineDivision of Microbiology and Molecular Medicine
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Transfusion
Pharmacology and Toxicology

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