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Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain
Umeå University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Pain and Rehabilitation Center. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Pain and Rehabilitation Center.
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2016 (English)In: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 6, no 1, 1-8 p.Article in journal (Refereed) Published
Abstract [en]

Proteomic screening analysis has detected myosin light chain (MLC) as a protein implied to be involved in chronic musculoskeletal neck and shoulder pain. Several analyses of MLC proteins have stated a difference in phosphorylation being the determining factor for protein activation hence altered contrability of the muscle in i.e. senescence. In continuation of a previous publication, this study is an attempt to analyze the different MLC isoforms by mass spectrometry and immune-analyses in myalgic and healthy trapezius muscle. In the present study no differences in phosphorylation level between the corresponding individual proteins were detected using LC-MSMS and immunoblotting; instead we assigned different isoforms of regulatory MLCs. To further elucidate the contrability: calcium (Ca2+) regulatory proteins, sarco(endo)plasmic reticulum Ca2+ ATPase 1 (SERCA-1) and calsequestrine (CSQ) were analyzed by western blot. The analysis revealed a significantly increased abundance of SERCA-1 protein in the myalgic muscle and a significantly increased abundance of CSQ in healthy muscle. Myalgic muscle contraction patterns have in previous studies shown to differ from healthy muscle which may be connected to the Ca2+ availability in the muscle. Here we present the proteomic characterization of differences in Ca2+ regulating proteins and particularly regulatory MLCs in trapezius muscle of women with chronic musculoskeletal neck and shoulder pain.

Place, publisher, year, edition, pages
2016. Vol. 6, no 1, 1-8 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-130539DOI: 10.5584/jiomics.v6i1.191OAI: oai:DiVA.org:liu-130539DiVA: diva2:952679
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2016-08-22

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Turkina, MariaCarlsson, AndersGerdle, BjörnLarsson, BrittGhafouri, Bijar
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Division of Cell BiologyFaculty of Medicine and Health SciencesDivision of Community MedicinePain and Rehabilitation Center
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