liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Detection limits of 405 nm and 633 nm excited PpIX fluorescence for brain tumor detection during stereotactic biopsy
Laser-Forschungslabor, LIFE-Zentrum, Klinikum der Universität München, Munich, Germany .
MRC Systems GmbH, Heidelberg, Germany.
Linköping University, Department of Biomedical Engineering, Biomedical Instrumentation. Linköping University, Faculty of Medicine and Health Sciences.ORCID iD: 0000-0002-0555-8877
Laser-Forschungslabor, LIFE-Zentrum, Klinikum der Universität München, Munich, Germany .
Show others and affiliations
2016 (English)In: Biophotonics: Photonic Solutions for Better Health Care V: Proceedings of SPIE / [ed] Jürgen Popp, Valery V. Tuchin, Dennis L. Matthews, Francesco S. Pavone, Brussels: SPIE - International Society for Optical Engineering, 2016, Vol. 9887, 98872ZConference paper, Poster (Refereed)
Abstract [en]

5-aminolevulinic-acid-(5-ALA)-induced protoporphyrin IX (PpIX) fluorescence may be used to improve stereotactic brain tumor biopsies. In this study, the sensitivity of PpIX-based tumor detection has been investigated for two potential excitation wavelengths (405 nm, 633 nm). Using a 200 μm fiber in contact with semi-infinite optical phantoms containing ink and Lipovenös, PpIX detection limits of 4.0 nM and 200 nM (relating to 1 mW excitation power) were determined for 405 nm and 633 nm excitation, respectively. Hence, typical PpIX concentrations in glioblastomas of a few μM should be well detectable with both wavelengths. Additionally, blood layers of selected thicknesses were placed between fiber and phantom. Red excitation was shown to be considerably less affected by blood interference: A 50 μm blood layer, for instance, blocked the 405- nm-excited fluorescence completely, but reduced the 633-nm-excited signal by less than 50%. Ray tracing simulations demonstrated that - without blood layer - the sensitivity advantage of 405 nm rises for decreasing fluorescent volume from 50-fold to a maximum of 100-fold. However, at a tumor volume of 1 mm3, which is a typical biopsy sample size, the 633-nm-excited fluorescence signal is only reduced by about 10%. Further simulations revealed that with increasing fiber-tumor distance, the signal drops faster for 405 nm. This reduces the risk of detecting tumor tissue outside the needle's coverage, but diminishes the overlap between optically and mechanically sampled volumes. While 405 nm generally offers a higher sensitivity, 633 nm is more sensitive to distant tumors and considerably superior in case of blood-covered tumor tissue.

Place, publisher, year, edition, pages
Brussels: SPIE - International Society for Optical Engineering, 2016. Vol. 9887, 98872Z
Series
, SPIE - International Society for Optical Engineering. Proceedings, ISSN 0277-786X, 1996-756X (electronic) ; 9887
Keyword [en]
5-aminolevulinic acid, Fluorescence spectroscopy, Glioblastoma multiforme, Optical phantoms, Protoporphyrin IX, Ray tracing simulations, Stereotactic biopsy
National Category
Other Medical Engineering
Identifiers
URN: urn:nbn:se:liu:diva-130962DOI: 10.1117/12.2225234ISBN: 9781510601321OAI: oai:DiVA.org:liu-130962DiVA: diva2:957377
Conference
Biophotonics: Photonic Solutions for Better Health Care V, Brussels, Belgium, April 4-7, 2016
Note

Funding agencies|German Ministry of Education and Research (BMBF)

Available from: 2016-09-01 Created: 2016-09-01 Last updated: 2016-09-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Haj Hosseini, Neda
By organisation
Biomedical InstrumentationFaculty of Medicine and Health Sciences
Other Medical Engineering

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 16 hits
ReferencesLink to record
Permanent link

Direct link