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Potential Pitfalls of the Mx1-Cre System: Implications for Experimental Modeling of Normal and Malignant Hematopoiesis
Lund University, Sweden; Lund University, Sweden.
Lund University, Sweden.
Lund University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Haematology. Lund University, Sweden; Lund University, Sweden; Skånes University Hospital, Sweden.
2016 (English)In: Stem Cell Reports, ISSN 2213-6711, Vol. 7, no 1, 21-28 p.Article in journal (Refereed) Published
Abstract [en]

Conditional knockout mice are commonly used to study the function of specific genes in hematopoiesis. Different promoters that drive Cre expression have been utilized, with the interferon-inducible Mx1-Cre still being the most commonly used "deleter strain in experimental hematology. However, different pitfalls associated with this system could lead to misinterpretation in functional studies. We present here two of these issues related to the use of Mx1-Cre: first, a high spontaneous recombination rate when applying commonly used techniques in experimental hematology, and second, undesired short-term consequences of the use of polyinosinic: polycytidylic acid, including changes in cellular phenotypes that, however, resolve within days. Our studies emphasize therefore that proper controls are crucial when modeling gene deletion using the Mx1-Cre transgene.

Place, publisher, year, edition, pages
CELL PRESS , 2016. Vol. 7, no 1, 21-28 p.
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-131187DOI: 10.1016/j.stemcr.2016.06.002ISI: 000380492300003PubMedID: 27373927OAI: oai:DiVA.org:liu-131187DiVA: diva2:971931
Note

Funding Agencies|Barncancerfonden; ERC [615068]; Vetenskapsradet

Available from: 2016-09-19 Created: 2016-09-12 Last updated: 2017-05-23

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Cammenga, Jörg
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Citation style
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