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2020 (English)In: Inflammatory Bowel Diseases, ISSN 1078-0998, E-ISSN 1536-4844, Vol. 26, no 7, p. 974-984Article in journal (Refereed) Published
Abstract [en]
Background
Barrier dysfunction is recognized as a pathogenic factor in ulcerative colitis (UC) and irritable bowel syndrome (IBS), but it is unclear to what extent the factors related to barrier dysfunction are disease-specific. The aim of this study was to compare these aspects in UC patients in remission, IBS patients, and healthy controls (HCs).
Methods
Colonic biopsies were collected from 13 patients with UC in remission, 15 patients with IBS-mixed, and 15 HCs. Ulcerative colitis patients had recently been treated for relapse, and biopsies were taken from earlier inflamed areas. Biopsies were mounted in Ussing chambers for measurements of intestinal paracellular permeability to 51chromium (Cr)-ethylenediaminetetraacetic acid (EDTA). In addition, biopsies were analyzed for mast cells and eosinophils by histological procedures, and plasma tumor necrosis factor (TNF)-α was assessed by ELISA.
Results
Ussing chamber experiments revealed an increased 51Cr-EDTA permeability in UC and IBS (P < 0.05). The 51Cr-EDTA permeability was higher in UC compared with IBS (P < 0.005). There were increased numbers of mucosal mast cells and eosinophils in UC and IBS and more eosinophils in UC compared with IBS (P < 0.05). Also, increased extracellular granule content was found in UC compared with HCs (P < 0.05). The 51Cr-EDTA permeability correlated significantly with eosinophils in all groups. Plasma TNF-α concentration was higher in UC compared with IBS and HCs (P < 0.0005).
Conclusions
Results indicate a more permeable intestinal epithelium in inactive UC and IBS compared with HCs. Ulcerative colitis patients, even during remission, demonstrate a leakier barrier compared with IBS. Both eosinophil numbers and activation state might be involved in the increased barrier function seen in UC patients in remission.
Place, publisher, year, edition, pages
Oxford University Press, 2020
Keywords
eosinophils; intestinal permeability; irritable bowel syndrome; mast cells; ulcerative colitis
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:liu:diva-167644 (URN)10.1093/ibd/izz328 (DOI)000544164200013 ()31944236 (PubMedID)2-s2.0-85083096809 (Scopus ID)
Note
Funding Agencies|Bengt Ihre research Scholarship; AFA research foundation; Bengt-Ihre fonden, County Council of Ostergotland; Swedish Research Council (VR-Medicine and Health)Swedish Research Council [2017-02475]; Swedish Foundation For Strategic ResearchSwedish Foundation for Strategic Research [RB13-016]; Instituto de Salud Carlos IIIInstituto de Salud Carlos III; Fondo de Investigacion SanitariaInstituto de Salud Carlos III [PI16/00583]; Ministerio de Ciencia, Innovacion y Universidades, Spain [CIBER-EHD CB06/04/0021]
2020-07-202020-07-202024-01-10Bibliographically approved