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  • 1.
    Comina, German
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Suska, Anke
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten.
    Filippini, Daniel
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska fakulteten.
    A 3D printed device for quantitative enzymatic detection using cell phones2016Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 8, nr 32, s. 6135-6142Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A disposable device for quantitative enzymatic detection capable of coupling illumination and image readouts from cell phones is demonstrated. The device integrates a calibration range for glutamate detection, utilizes the phone screen as a light source, and provides the necessary actuation for autonomous operation. Custom made optics required to couple to the cell phone camera is accomplished using affordable stereolithography (SLA) 3D printers. The described method does not involve polishing, requires only two steps from design to implementation, and can be locally applied to 3D printed lab-on-a-chip (LOC) prototypes, using the same materials. Optical finishing and dimensional variability within 2% were achieved, supporting entirely arbitrary geometries for elements larger than 400 mm in radius. Representative fabrication times and costs were 20 min and $0.50 USD per prototype.

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  • 2.
    Harris, L. F.
    et al.
    Dublin Institute Technology, Ireland.
    Rainey, P.
    Queens University of Belfast, North Ireland.
    Lindahl, Tomas
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Diagnostikcentrum, Klinisk kemi.
    Killard, A. J.
    University of West England, England.
    A fully integrated microfluidic device for point of care monitoring of antithrombotics2016Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 8, nr 35, s. 6500-6505Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The simplicity and efficiency of point of care diagnostics have revolutionised patient care. Current methods for measuring hypercoagulability often require trained technicians, large blood volumes, and result in long turnaround times. Standard testing for hypercoagulable disorders is performed in the central laboratory using automated coagulation analysers. However the trend is moving towards the development and implementation of point of care testing, as a result of the ever increasing number of patients on antithrombotic therapy. We present a novel microfluidic device and assay for monitoring the effect of two anticoagulants, unfractionated heparin (UFH) and low molecular weight heparin (LMWH). The assay is based on the anti-Xa assay principle but uses fluorescence detection. Our device is a disposable laminate microfluidic strip, fabricated from the cyclic polyolefin (COP), Zeonor (R), which is extremely suitable for application to fluorescent device platforms. We present data on the execution of the anti-Xa assay in this microfluidic format, demonstrating that the assay can be used to measure both UFH and LMWH in human plasma samples from 0 to 1 U mL(-1), with a rapid result obtained within 30-60 seconds.

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  • 3.
    Li, Shuai
    et al.
    Xiamen Univ, Peoples R China.
    Li, Yaoxuan
    Shanxi Med Univ, Peoples R China.
    Zhang, Shiji
    Xiamen Univ, Peoples R China.
    Fang, Haixiao
    Xiamen Univ, Peoples R China; Future Display Inst Xiamen, Peoples R China.
    Huang, Ze
    Xiamen Univ, Peoples R China.
    Zhang, Duoteng
    Xiamen Univ, Peoples R China.
    Ding, Aixiang
    Xiamen Univ, Peoples R China.
    Uvdal, Kajsa
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär ytfysik och nanovetenskap. Linköpings universitet, Tekniska fakulteten.
    Hu, Zhang-Jun
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär ytfysik och nanovetenskap. Linköpings universitet, Tekniska fakulteten.
    Huang, Kai
    Future Display Inst Xiamen, Peoples R China.
    Li, Lin
    Xiamen Univ, Peoples R China; Future Display Inst Xiamen, Peoples R China.
    Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection2024Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Temperature homeostasis is critical for cells to perform their physiological functions. Among the diverse methods for temperature detection, fluorescent temperature probes stand out as a proven and effective tool, especially for monitoring temperature in cells and suborganelles, with a specific emphasis on mitochondria. The utilization of these probes provides a new opportunity to enhance our understanding of the mechanisms and interconnections underlying various physiological activities related to temperature homeostasis. However, the complexity and variability of cells and suborganelles necessitate fluorescent temperature probes with high resolution and sensitivity. To meet the demanding requirements for intracellular/subcellular temperature detection, several strategies have been developed, offering a range of options to address this challenge. This review examines four fundamental temperature-response strategies employed by small molecule and polymer probes, including intramolecular rotation, polarity sensitivity, Forster resonance energy transfer, and structural changes. The primary emphasis was placed on elucidating molecular design and biological applications specific to each type of probe. Furthermore, this review provides an insightful discussion on factors that may affect fluorescent thermometry, providing valuable perspectives for future development in the field. Finally, the review concludes by presenting cutting-edge response strategies and research insights for mitigating biases in temperature sensing. In this review, we primarily summarized four temperature-response strategies. Then, we further analyzed the chemical modifications and biological applications of the probes. Finally, we have provided a prospective on the future development of probes.

  • 4.
    Nasef, Hany
    et al.
    Universitat Rovira i Virgili, Tarragona, Spain .
    Beni, Valerio
    Universitat Rovira i Virgili, Tarragona, Spain .
    O´Sullivan, Ciara K.
    Universitat Rovira i Virgili, Tarragona, Spain .
    Labelless electrochemical melting curve analysis for rapid mutation detection2010Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 2, nr 10, s. 1461-1466Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In the post-genome era there is an increasing demand for cost effective and rapid methods for thedetection of specific mutations and single nucleotide polymorphisms. Here we describe a method forthe rapid detection of mutations exploiting labelless electrochemical melting curve analysis, using thedetection of the cystic fibrosis associated DF508 mutant as a model. A 21-base long thiolated probe,complementary to the region of Exon 10 of the cystic fibrosis transmembrane regulatory gene where theDF508 mutation lies, was immobilised on a gold electrode and hybridised to a synthetic analogue ofsingle stranded PCR products for each of the mutant (85 bases) and wild type (82 bases) targets.Experimental conditions were optimised to exploit the guanine-specific interaction of the electroactiveindicator, methylene blue. Upon hybridisation of the immobilised probe to the target, the number ofguanine bases present in close proximity with the sensor surface increased from 3 to 14, resulting ina significant increase in signal. Ramping of the temperature caused denaturation of the on-surfaceimmobilised duplex and a concomitant reduction in signal. From the first derivative of the meltingcurves a clear differentiation between the mutant and wild-type target could be observed. The proposedapproach can be extended to array based melting curve analysis, allowing the simultaneous detection ofmultiple mutations and SNPs, and moreover the melting properties observed can also be used to designgenosensors for single target detection.

  • 5.
    Razmi, Nasrin
    et al.
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik, elektroteknik och matematik. Linköpings universitet, Tekniska fakulteten.
    Hasanzadeh, Mohammad
    Tabriz Univ Med Sci, Iran.
    Willander, Magnus
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik, elektroteknik och matematik. Linköpings universitet, Tekniska fakulteten.
    Nur, Omer
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik, elektroteknik och matematik. Linköpings universitet, Tekniska fakulteten.
    Electrochemical genosensor based on gold nanostars for the detection of Escherichia coli O157:H7 DNA2022Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 14, nr 16, s. 1562-1570Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Escherichia coli O157:H7 (E. coli O157:H7) is an enterohemorrhagic E. coli (EHEC), which has been issued as a major threat to public health worldwide due to fatal contamination of water and food. Thus, its rapid and accurate detection has tremendous importance in environmental monitoring and human health. In this regard, we report a simple and sensitive electrochemical DNA biosensor by targeting Z3276 as a genetic marker in river water. The surface of the designed gold electrode was functionalized with gold nanostars and an aminated specific sensing probe of E. coli O157:H7 to fabricate the genosensor. Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were applied for electrochemical characterization and detection. The synthesized gold nanostars were characterized using different characterization techniques. The fabricated DNA-based sensor exhibited a high selective ability for one, two, and three-base mismatched sequences. Regeneration, stability, selectivity, and kinetics of the bioassay were investigated. Under optimal conditions, the fabricated genosensor exhibited a linear response range of 10(-5) to 10(-17) mu M in the standard sample and 7.3 to 1 x 10(-17) mu M in water samples with a low limit of quantification of 0.01 zM in water samples. The detection strategy based on silver plated gold nanostars and DNA hybridization improved the sensitivity and specificity of the assay for E. coli O157:H7 detection in real water samples without filtration. The detection assay has the advantages of high selectivity, sensitivity, low amounts of reagents, short analysis time, commercialization, and potential application for the determination of other pathogenic bacteria.

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  • 6.
    Tran, Thuy
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biofysik och bioteknik. Linköpings universitet, Tekniska fakulteten.
    Martinsson, Erik
    ArgusEye AB, Spannmalsgatan 55, S-58336 Linkoping, Sweden.
    Gustavsson, Robert
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biofysik och bioteknik. Linköpings universitet, Tekniska fakulteten.
    Tronarp, Otto
    Wolfram MathCore AB, Teknikringen 1E, S-58330 Linkoping, Sweden.
    Nilsson, Mats
    BioInvent Int AB, Sweden.
    Hansson, Kristoffer Rudenholm
    BioInvent Int AB, Sweden.
    Lundström, Ingemar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensor- och aktuatorsystem. Linköpings universitet, Tekniska fakulteten.
    Mandenius, Carl-Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biofysik och bioteknik. Linköpings universitet, Tekniska fakulteten.
    Aili, Daniel
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biofysik och bioteknik. Linköpings universitet, Tekniska fakulteten.
    Process integrated biosensors for real-time monitoring of antibodies for automated affinity purification2022Ingår i: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 14, nr 44, s. 4555-4562Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Therapeutic monoclonal antibodies (mAbs) provide new means for treatments of a wide range of diseases and comprise a large fraction of all new approved drugs. Production of mAbs is expensive compared to conventional drug production, primarily due to the complex processes involved. The affinity purification step is dominating the cost of goods in mAb manufacturing. Process intensification and automation could reduce costs, but the lack of real-time process analytical technologies (PAT) complicates this development. We show a specific and robust fiber optical localized surface plasmon resonance (LSPR) sensor technology that is optimized for in-line product detection in the effluent in affinity capture steps. The sensor system comprises a flow cell and a replaceable sensor chip functionalized with biorecognition elements for specific analyte detection. The high selectivity of the sensor enable detection of mAbs in complex sample matrices at concentrations below 2.5 mu g mL(-1). In place regeneration of the sensor chips allowed for continuous monitoring of multiple consecutive chromatographic separation cycles. Excellent performance was obtained at different purification scales with flow rates up to 200 mL min(-1). This sensor technology facilitates efficient column loading, optimization, and control of chromatography systems, which can pave the way for continuous operation and automation of protein purification steps.

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