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  • 1.
    Balau, Luminita
    et al.
    Department of Physical - Chemistry, Technical University, Gh. Asachi, Iasi, Romania.
    Lisa, Gabriela
    Department of Physical - Chemistry, Technical University, Gh. Asachi, Iasi, Romania.
    Popa, M. I.
    Department of Physical - Chemistry, Technical University, Gh. Asachi, Iasi, Romania.
    Tura, V.
    Faculty of Physics, University, Al. I. Cuza, Iasi, Romania.
    Melnig, V.
    Faculty of Physics, University, Al. I. Cuza, Iasi, Romania.
    Physico-chemical properties of Chitosan films2004In: Central European Journal of Chemistry, ISSN 1895-1066, E-ISSN 1644-3624, Vol. 2, no 4, p. 638-647Article in journal (Refereed)
    Abstract [en]

    Chitosan films obtained by dry phase inversion were prepared from an aqueous solution of chitosan in acetic acid. The films, of thickness less than 20 μm, were transparent, very flexible and had smooth surfaces. Increasing the film thickness induced an increase of the internal tensions and the consequent formation of a rough surface. Structural investigations by X-ray diffraction and Fourier transform IR analysis, showed that the chitosan films, as prepared, are amorphous. Further annealing to evaporate acetic acid and water traces, changed the amorphous phase into a more ordered phase, characterized by diffraction peaks at 2θ values of 9, 17, 20 and 23 degrees. Thermal investigations by TG, DTG, and DTA revealed that the decomposition of the chitosan films as prepared proceeds in two stages, starting from 180°C and 540°C.

  • 2.
    Baranowska, Irena
    et al.
    Silesian Technical University, Gliwice, Poland.
    Magiera, Sylwia
    Silesian Technical University, Gliwice, Poland.
    Baranowski, Jacek
    Östergötlands Läns Landsting, Heart and Medicine Centre, Department of Clinical Physiology UHL.
    Use of multivariate statistical techniques to optimize the separation of isoflavones by liquid chromatography2011In: Central European Journal of Chemistry, ISSN 1895-1066, E-ISSN 1644-3624, Vol. 9, no 6, p. 972-981Article in journal (Refereed)
    Abstract [en]

    The aim of this paper is to optimize and validate a high performance liquid chromatography (HPLC) method for separation and quantification of five isoflavones. A statistical central composite design was used to separate all peaks. These multivariate procedures were efficient in determining the optimal separation condition using resolution, capacity factor, asymmetry and number of theoretical plates. less thanbrgreater than less thanbrgreater thanThe effective separation of the examined compounds was applied on a Develosil RP Aqueous AR 5 RP-30 column with a gradient mobile phase system and a DAD detector. less thanbrgreater than less thanbrgreater thanThe isolation and preconcentration of the isoflavones from urine and plasma samples were conducted by means of the solid-phase extraction (SPE). For optimize SPE conditions various sorbents were tested. Furthermore, high recoveries and good relative standard deviations were obtained when the samples were passed through the Oasis HLB column. The developed method was validated and successfully applied for determination of isoflavones in urine and plasma.

  • 3.
    Nikoleli, Georgia-Paraskevi
    et al.
    National Technical University of Athens, Greece .
    Hussain Ibupoto, Zafar
    Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
    Nikolelis, Dimitrios P.
    University of Athens, Greece .
    Likodimos, Vlassis
    NCSR Demokritos, Greece .
    Psaroudakis, Nikolas
    University of Athens, Greece .
    Tzamtzis, Nikolaos
    National Technical University of Athens, Greece .
    Willander, Magnus
    Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
    Hianik, Tibor
    Comenius University, Slovakia .
    Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane2013In: Central European Journal of Chemistry, ISSN 1895-1066, E-ISSN 1644-3624, Vol. 11, no 9, p. 1554-1561Article in journal (Refereed)
    Abstract [en]

    A novel potentiometric cholesterol biosensor has been fabricated through the immobilization of the stabilized polymeric lipid membrane onto graphene electrode. The stabilized polymeric lipid membrane is composed of cholesterol oxidase enzyme and polymerization mixture; which holds paramount influence on the properties of the cholesterol biosensor. The presented biosensor reveals an appreciable reproducibility, good selectivity and high sensing capability with a linear slope curve of similar to 64 mV per decade. The strong biocompatibility among stabilized polymeric lipid membranes and human biofluids provides the possibility to use for real blood samples and other biological applications.

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