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  • 1.
    Ali Kamyabi, Mohammad
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Hajari, Nasim
    University of Zanjan, Iran .
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    A high-performance glucose biosensor using covalently immobilised glucose oxidase on a poly(2,6-diaminopyridine)/carbon nanotube electrode2013In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 116, p. 801-808Article in journal (Refereed)
    Abstract [en]

    A highly-sensitive glucose biosensor amenable to ultra-miniaturisation was fabricated by immobilisation of glucose oxidase (GOx), onto a poly(2,6-diaminopyridine)/multi-walled carbon nanotube/glassy carbon electrode (poly(2,6-DP)/MWNT/GCE). Cyclic voltammetry was used for both the electrochemical synthesis of poly-(2,6-DP) on the surface of a MWNT-modified GC electrode, and characterisation of the polymers deposited on the GC electrode. The synergistic effect of the high active surface area of both the conducting polymer, i.e., poly-(2,6-DP) and MWNT gave rise to a remarkable improvement in the electrocatalytic properties of the biosensor. The transfer coefficient (alpha), heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.6, 4 s(-1) and 0.20 mM at pH 7.4, respectively. The GOx/poly(2,6-DP)/MWNT/GC bioelectrode exhibited two linear responses to glucose in the concentration ranging from 0.42 mu M to 8.0 mM with a correlation coefficient of 0.95, sensitivity of 52.0 mu AmM-1 cm(-2), repeatability of 1.6% and long-term stability, which could make it a promising bioelectrode for precise detection of glucose in the biological samples. (C) 2013 Elsevier B.V. All rights reserved.

  • 2.
    Ali Kamyabi, Mohammad
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Zanjan University, Iran.
    Hajari, Nasim
    Zanjan University, Iran.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Correction: A high-performance glucose biosensor using covalently immobilised glucose oxidase on a poly(2,6-diaminopyridine)/carbon nanotube electrode (vol 116, pg 801, 2013)2016In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 153, p. 414-415Article in journal (Refereed)
    Abstract [en]

    A highly-sensitive glucose biosensor amenable to ultraminiaturisation was fabricated by immobilization of glucose oxidase (wGOX), onto a poly(2,6-diaminopyridine)/multi-walled carbon nanotube/glassy carbon electrode (poly(2,6-DP)/MWCNT/GCE). Cyclic voltammetry was used for both the electrochemical synthesis of poly-(2,6-DP) on the surface of a MWCNT-modified GC electrode, and characterization of the polymers deposited on the GC electrode. The synergistic effect of the high active surface area of both the conducting-polymer, i.e., poly-(2,6-DP) and MWCNT gave rise to a remarkable improvement in the electrocatalytic properties of the biosensor. The transfer coefficient (alpha), heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.6, 4 s-1 and 0.22 mM at pH 7.4, respectively. The GOx/poly(2,6-DP)/MWCNT/GC bioelectrode exhibited two linear responses to glucose in the concentration ranging from 0.42 mu M to 8.0 mM with a correlation coefficient of 0.95, sensitivity of 52.0 mu AmM-1 cm-2, repeatability of 1.6% and long-term stability, which could make it a promising bioelectrode for precise detection of glucose in the biological samples. (C) 2016 Elsevier B.V. All rights reserved.

  • 3.
    Ashaduzzaman, Md
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Dhaka, Bangladesh.
    Anto Antony, Aswathi
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Murugan, N. Arul
    Royal Institute Technology, Sweden.
    Deshpande, Swapneel R.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Tekidag AB, UCS, S-58330 Linkoping, Sweden.
    Studies on an on/off-switchable immunosensor for troponin T2015In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73, p. 100-107Article in journal (Refereed)
    Abstract [en]

    Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (Delta G(coil)=-6.0 Kcal/mole) and 37 degrees C (Delta G(globular)=-41.0 kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with built-in programmable interactions of antigen-antibody upon receiving stimuli.

  • 4. Ashaduzzaman, Md
    et al.
    Parlak, Onur
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Fabrication of on/off-switchable enzymatic bioreactor for smart bioelectronics.2015In: Sweden-Japan Seminar on Nanomaterials and Nanotechnology – SJS-Nano, Linköping, Sweden, 10-11 March 2015., Japan Society for the Promotion of Science (JSPS), Stockholm , 2015, p. 36-37Conference paper (Refereed)
  • 5.
    Chey, Chan Oeurn
    et al.
    Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
    Patra, Hirak K
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tengdelius, Mattias
    Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
    Golabi, Mohsen
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Parlak, Onur
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Imani, Roghayeh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Elhag, Sami A. I.
    Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
    Yandi, Wetra
    Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Impact of nanotoxicology towards technologists to end users2013In: Advanced Materials Letters, ISSN 0976-3961, E-ISSN 0976-397X, Vol. 4, no 8, p. 591-597Article in journal (Refereed)
    Abstract [en]

    The length scale for nanomaterial is small enough to be invisible and presume innocence for the initial avoidance of the toxicity issues. Again it was beyond the understanding of the time frame when nanotechnology just blooms that a length scale itself might be an important toxic parameter apart from its materialistic properties. We present this report to address the fundamental issues and questions related to the nanotoxicity issues from laboratory to the land of applications. We emphasize about the basic nanoscale materials that are regularly being used by the scientific community and the nanotechnology based materials that has already in the market or will come soon.

  • 6.
    Imani, Roghayeh
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Iglič, Aleš
    Biophysics Laboratory, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia.
    Turner, Anthony P.F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Electrochemical detection of DNA damage through visible-light-induced ROS using mesoporous TiO2 microbeads2014In: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 40, p. 84-87Article in journal (Refereed)
    Abstract [en]

    Rapid detection of DNA damage could serve as a basis for genotoxicity studies of new bio-nanoconjugations. A novel TiO2 bio-nanoconjugation, consisting of mesoporous TiO2 microbeads, dopamine (DA) and ss-DNA, was constructed on fluorine-doped tin oxide-coated glass (FTO) and used for the detection of DNA damage in the photocatalytic reaction of TiO2 under visible light. Stable mesoporous TiO2 microbeads films were coated on FTO by the doctor-blade method; dopamine with oxygen containing ligands, was tightly coupled to the titanium surface prepaired under phase coordination. Specific single-strands of DNA were electronically linked to TiO2 by using a dopamine bridge. DNA damage, caused by reactive oxygen species (ROS) that were photogenerated through the photocatalytic reaction, was detected with square wave voltammetry (SWV) by recording the catalytic oxidation current of [Ru(NH3)6]3 +, an intercalated electroactive probe. The ability of antioxidant to protect DNA against damage in the photocatalytic reaction was also tested.

  • 7.
    Karimian, N
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Zavar, M H A
    Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    Chamsaz, M
    Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    On/off-switchable electrochemical sensor for folicacid based on molecularly imprinted technology.2013In: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 36, p. 92-95Article in journal (Refereed)
    Abstract [en]

    The combination of smart polymers with molecular imprinting offers a powerful tool to design more effective sensors and medical devices. In this study, a temperature sensitive amine-terminated poly(N-isopropylacrylamide) block with (N,N'-methylenebisacrylamide) cross-linker along with o-phenylenediamine was electropolymerised on a gold electrode in the presence of folic acid (FA) as template to produce an on/off-switchable molecularly imprinted polymer (MIP) affinity sensor for folic acid. Differential pulse voltammetry and cyclic voltammetry were used to characterise the FA-imprinted layer. Incubation of the MIP-modified electrode with FA resulted in a suppression of the ferro/ferricyanide redox process. The highest sensitivity of this temperature gated on/off-switchable folic acid sensor was achieved at 22 °C. Such switchable affinity materials offer considerable potential for the design of highly selective and controllable biosensors and immunoassays.

  • 8.
    Karimian, Najmeh
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. Ferdowsi University of Mashhad, Iran.
    Hossein Arbab Zavar, Mohammad
    Ferdowsi University of Mashhad, Iran.
    Chamsaz, Mahmoud
    Ferdowsi University of Mashhad, Iran.
    Ashraf, Narges
    Ferdowsi University of Mashhad, Iran.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. Tekidag AB, UCS, Linköping, Sweden.
    A potential-gated molecularly imprinted smart electrode for nicotinamide analysis2015In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 5, no 44, p. 35089-35096Article in journal (Refereed)
    Abstract [en]

    Triggered surface responsiveness paves the way for smart sensor technologies that not only have tunable retention, but also provide sensing through a built-in programming of electrode material. In this study, we report a potential-gated electrochemical sensor for determination of nicotinamide (NAM) based on a molecularly imprinted overoxidised polypyrrole electrode. The sensitive layer was prepared by electropolymerisation of pyrrole on a glassy carbon electrode in the presence of NAM as a template molecule, followed by alkali extraction. Electrochemical methods were used to monitor the processes of electropolymerisation, template removal and binding in the presence of a [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-) redox couple as an electrochemical probe. Several factors affecting the performance of the MIP-modified electrode were investigated and optimized. The peak current of the ferro/ferricyanide couple decreased linearly with successive addition of NAM in the concentration range 0.9 x 10(-6) to 9.9 x 10(-3) M with a detection limit of 1.7 x 10(-7) M (S/N = 3). The molecularly-imprinted polymer (MIP) electrode had excellent recognition capability for NAM compared to structurally related molecules. Moreover, the reproducibility and repeatability of the NAM-imprinted electrode were all found to be satisfactory. The results from sample analysis confirmed the applicability of the NAM-imprinted electrode to reusable quantitative analysis in commercial pharmaceutical samples.

  • 9.
    Karimian, Najmeh
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. Ferdowsi University of Mashhad, Mashhad, Iran.
    Turner, Anthony P.F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Electrochemical evaluation of troponin T imprinted polymer receptor2014In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 59, p. 160-165Article in journal (Refereed)
    Abstract [en]

    The selective detection and quantification of macromolecular targets is a fundamental biological mechanism in nature. Molecularly imprinted polymers (MIPs) have been identified as one of the most promising synthetic alternatives to bioreceptors. However, expanding this methodology towards selective recognition of bulky templates such as proteins appears to be extremely challenging due to problems associated with removal of the template from the polymeric network. In this study, polymer imprinted with troponin T (TnT) was assessed using electrochemical methods and the influence of various extraction methods, including conventional immersion extraction, thermal annealing and ultrasonic-assisted extraction, on the binding characteristics of the troponin-to-imprinted polymer receptor was elucidated. Cyclic voltammetric deposition of o-phenylenediamine (o-PD) film in the presence of TnT as a template was performed in acetate buffer (0.5 M, pH 5.2) on a gold substrate. Solvent extraction of the target molecule was optimised and followed by subsequent washing with water. The electrochemistry of a ferro/ferricyanide probe was used to characterise the TnT MIP receptor film. The incubation of the TnT MIP receptor-modified electrode with respect to TnT concentration resulted in a suppression of the ferro/ferricyanide redox current. The dissociation constant (KD) was calculated using a two-site model of template affinity for the TnT MIP receptor. The synthetic TnT MIP receptor had high affinity for TnT with a KD of 2.3×10−13 M.

  • 10.
    Karimian, Najmeh
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. Ferdowsi University of Mashhad, Iran.
    Vagin, Mikhail
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Hossein Arbab Zavar, Mohammad
    Ferdowsi University of Mashhad, Iran .
    Chamsaz, Mahmoud
    Ferdowsi University of Mashhad, Iran .
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    An ultrasensitive molecularly-imprinted human cardiac troponin sensor2013In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 50, p. 492-498Article in journal (Refereed)
    Abstract [en]

    Cardiac troponin T (TnT) is a highly sensitive cardiac biomarker for myocardial infarction. In this study, the fabrication and characterisation of a novel sensor for human TnT based on a molecularly-imprinted electrosynthesised polymer is reported. A TnT sensitive layer was prepared by electropolymerisation of o-phenylenediamine (o-PD) on a gold electrode in the presence of TnT as a template. To develop the molecularly imprinted polymer (MIP), the template molecules were removed from the modified electrode surface by washing with alkaline ethanol. Electrochemical methods were used to monitor the processes of electropolymerisation, template removal and binding. The imprinted layer was characterised by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM). The incubation of the MIP-modified electrode with respect to TnT concentration resulted in a suppression of the ferro/ferricyanide redox process. Experimental conditions were optimised and a linear relationship was observed between the peak current of [Fe(CN)(6)](3-)/[Fe (CN)(6)](4-) and the concentration of TnT in buffer over the range 0.009-0.8 ng/mL, with a detection limit of 9 pg/mL. The TnT MIP sensor was shown to have a high affinity to TnT in comparison with nonimprinted polymer (NIP) electrodes in both buffer and blood serum.

  • 11.
    Karimiana, Naymeh
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Zavarb, M H A
    Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    Chamsazb, M
    Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    On/off-switchable folic acid sensor using molecularly imprinted smart polymer electrode2014In: 24thAnniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper (Other academic)
    Abstract [en]

    Recently, much attention has been focused on the development of controlled switchable surfaces, also known as “smart surfaces”, which switch their physicochemical properties in response to external stimuli [1]. Switching of a surface based on temperature can be realised using thermo-sensitive polymers, which undergo a phase transition at the lower critical solution temperature (LCST), where their behavior switches between hydrophobic and hydrophilic [2]. LCST modulation can be achieved by copolymerisation with other monomers in order to produce a LCST close to physiological temperature. Thus, it could be useful in controllable, temperature-responsive bio-switches for biomedical and biotechnology applications [3]. The combination of smart polymers with molecular imprinting offers a powerful tool to design more effective sensors and medical devices. In this study, a temperature sensitive amine-terminated poly(N-isopropylacrylamide) block with (N,N'-methylenebisacrylamide) cross-linker along with o-phenylenediamine was electropolymerised on a gold electrode in the presence of folic acid (FA) as template to produce an on/off-switchable molecularly imprinted polymer (MIP) affinity sensor for folic acid. Differential pulse voltammetry and cyclic voltammetry were used to characterise the FA-imprinted layer. Incubation of the MIP-modified electrode with FA resulted in a suppression of the ferro/ferricyanide redox process. The highest sensitivity of this temperature gated on/off-switchable folic acid sensor was achieved at 22 ºC. Such switchable affinity materials offer considerable potential for the design of highly selective and controllable biosensors and immunoassays.

  • 12.
    Kashefi-Kheyrabadi, Leila
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. University of Isfahan, Iran.
    Mehrgardi, Masoud
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. University of Isfahan, Iran.
    Wiechec, Emilia
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences.
    Turner, Anthony P.F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Ultrasensitive detection of human liver hepatocellular carcinoma (HepG2) cells using a label-free aptasensor2014In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 86, no 10, p. 4956-4960Article in journal (Refereed)
    Abstract [en]

    Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast and colon tumours. The aptasensor delivered a wide linear dynamic range over 1 × 102 – 1 × 106 cell/mL, with a detection limit of 2 cell/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.

  • 13.
    Kumari, Poonam
    et al.
    School of Physics, Shoolini University, Solan, HP, India .
    Rai, Radheshyam
    School of Physics, Shoolini University, Solan, HP, India .
    Kholkin, Anderi L.
    Department of glass and ceramics, Aveiro University, Portugal.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Study of Ca doping on A- site on the structural and physical properties of BLTMNZ ceramics2014In: Advanced Materials Letters, ISSN 0976-3961, E-ISSN 0976-397X, Vol. 5, no 5, p. 255-259Article in journal (Refereed)
    Abstract [en]

    The ferroelectric Ca doped (Ba0.9575La0.04X0.0025) (Ti0.815Mn0.0025Nb0.0025Zr0.18)0.99O3 was prepared by a high-temperature solid state reaction technique. For the understanding of the electrical and dielectric property, the relation between the crystal structures, electrical transition and ferroelectric transitions with increasing temperature (–160 to 35°C) have been analyzed. X-ray diffraction analysis of the powders suggests the formation of a single-phase material with monoclinic structure. Capacitance and tanδ of the specimens were measured in the temperature range from -160 to 35°Cat frequencies 1 kHz – 1 MHz. Detailed studies of dielectric and electrical properties indicate that the Curie temperature shifted to higher temperature with the increase in frequency. Moreover, the dielectric maxima dropped down rapidly initially and the dielectric peaks became extremely broad. The AC conductivity increases with increase in frequency. The low value of activation energy obtained for the ceramic samples could be attributed to the influence of electronic contribution to the conductivity.

  • 14.
    Li, Songjun
    et al.
    Cranfield University.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, The Institute of Technology.
    Dedication and prominence: 31 years in ‘Biosensors and bioelectronics’: Dedicated to Professor Anthony P.F. Turner’s 61st birthday in Advanced Materials Letters2011In: Advanced Materials Letters, ISSN 0976-3961, E-ISSN 0976-397X, Vol. 2, no 2, p. 84-89Article in journal (Other academic)
    Abstract [en]

    The name Anthony P. F. Turner, biosensor pioneer, is often considered synonymous with his chosen field. This 5th June will be his 61st birthday. We track here his professional footprint, in order to celebrate his upcoming birthday and to commemorate his 31-years of dedication to biosensors. Commemorating this pioneer‟s achievements is a multidisciplinary celebration of his prominent contribution to biotechnology, chemistry, biomaterials and nanotechnology.

  • 15.
    Mishra, Ajay Kumar
    et al.
    UJ Nanomaterials Science Research Group, Department of Chemical Technology, University of Johannesburg, P.O. Box 17011, Doornfontien 2028, Johannesburg, South Africa.
    Mishra, Shivani B.
    UJ Nanomaterials Science Research Group, Department of Chemical Technology, University of Johannesburg, P.O. Box 17011, Doornfontien 2028, Johannesburg, South Africa.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Polymers/Composites Based Intelligent Transducers2012In: Intelligent Nanomaterials: processes, properties, and applications / [ed] Ashutosh Tiwari, Ajay Kumar Mishra, Hisatoshi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 571-584Chapter in book (Other academic)
    Abstract [en]

    The last three decades have seen extraordinary advances in the generation of new materials based on both fundamental elements and composites, driven by advances in synthetic chemistry and often drawing inspiration from nature. The concept of an intelligent material envisions additional functionality built into the molecular structure, such that a desirable response occurs under defined conditions.

    Divided into 4 parts: Inorganic Materials; Organic Materials; Composite Materials; and Biomaterials, the 22 chapters cover the latest research and developments in the processing, properties, and applications of intelligent nanomaterials. Included are molecular device materials, biomimetic materials, hybrid-type functionalized polymers-composite materials, information-and energy-transfer materials, as well as environmentally friendly materials.

  • 16.
    Mishra, Sachin
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of the Free State, Bloemfontein, South Africa.
    Ashaduzzaman, M.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Dhaka, Dhaka, Bangladesh..
    Mishra, Prashant
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of the Free State, Bloemfontein, South Africa.
    Swart, H.C.
    Department of Physics, University of the Free State, Bloemfontein, South Africa.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Vinoba Bhave Research Institute, Sirsa Road, Saidabad, Allahabad, India.
    Stimuli-enabled zipper-like graphene interface for auto-switchable bioelectronics.2017In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 89, p. 305-311Article in journal (Refereed)
    Abstract [en]

    Graphene interfaces with multi-stimuli responsiveness are of particular interest due to their diverse super-thin interfacial behaviour, which could be well suited to operating complex physiological systems in a single miniaturised domain. In general, smart graphene interfaces switch bioelectrodes from the hydrophobic to hydrophilic state, or vice versa, upon triggering. In the present work, a stimuli encoded zipper-like graphene oxide (GrO)/polymer interface was fabricated with in situ poly(N-isopropylacrylamide–co–diethylaminoethylmethylacrylate), i.e., poly(NIPAAm–co–DEAEMA) directed hierarchical self-assembly of GrO and glucose oxidase (GOx). The designed interface exhibited reversible on/off-switching of bio-electrocatalysis on changing the pH between 5 and 8, via phase transition from super hydrophilic to hydrophobic. The study further indicated that the zipper-like interfacial bioelectrochemical properties could be tuned over a modest change of temperature (i.e., 20–40 °C). The resulting auto-switchable interface has implications for the design of novel on/off-switchable biodevices with ‘in-built’ self-control.

  • 17.
    Mondal, Debasish
    et al.
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Electrospun Nanomatrix for Tissue Regeneration2012In: Biomedical Materials and Diagnostic Devices / [ed] Ashutosh Tiwari, Murugan Ramalingam, Hisashi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 577-596Chapter in book (Other academic)
    Abstract [en]

    The functional materials with the most promising outlook have the ability to precisely adjust the biological phenomenon in a controlled mode. Engineering of advanced bio- materials has found striking applications in used for biomedical and diagnostic device applications, such as cell separation, stem-cell, drug delivery, hyperthermia, automated DNA extraction, gene targeting, resonance imaging, biosensors, tissue engineering and organ regeneration

  • 18.
    Mondal, Debasish
    et al.
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Nanofibers Based Biomedical Devices2012In: Intelligent Nanomaterials: processes, properties, and applications / [ed] Ashutosh Tiwari, Ajay Kumar Mishra, Hisatoshi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 679-714Chapter in book (Other academic)
    Abstract [en]

    The last three decades have seen extraordinary advances in the generation of new materials based on both fundamental elements and composites, driven by advances in synthetic chemistry and often drawing inspiration from nature. The concept of an intelligent material envisions additional functionality built into the molecular structure, such that a desirable response occurs under defined conditions.

    Divided into 4 parts: Inorganic Materials; Organic Materials; Composite Materials; and Biomaterials, the 22 chapters cover the latest research and developments in the processing, properties, and applications of intelligent nanomaterials. Included are molecular device materials, biomimetic materials, hybrid-type functionalized polymers-composite materials, information-and energy-transfer materials, as well as environmentally friendly materials.

  • 19.
    Nordin, Anis
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Revuri, Vishnu
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Bhowmick, Neil
    Cedars-Sinai Medical Center, Los Angeles, USA.
    Voiculescu, Ioana
    City College of New York, USA.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Label-free Detection of Prostate Cancer Biomarker2014In: 24th Anniversary World Congress on Biosensors – Biosensors 2014 / [ed] Anthony Turner, Elsevier, 2014Conference paper (Other academic)
    Abstract [en]

    Prostate cancer is common and a frequent cause of cancer death, especially for American men. Most prostate cancers progress very slowly and while on the average about 30% of American males develop prostate cancer, only 3% die from the disease.  The disparity in the statistics indicates that a more effective screening method is required to differentiate the aggressive form of prostate cancer that causes mortality in patients. In this work, a label-free, novel microelectromechanical (MEMS) biosensor for detection of cysteine, a prostate cancer biomarker is presented. This biosensor merges two biosensing techniques, namely resonant frequency measurements and electrochemical impedance spectroscopy (EIS) on a single biosensor. The sensor is based on the innovative placement of the working microelectrodes for EIS technique as the top electrode of a quartz crystal microbalance (QCM) resonator. The QCM acoustic wave sensor consists of a thin AT-cut quartz substrate with two gold electrodes on both sides. The top metal electrode used for generating the acoustic wave is also used for EIS measurements of the biosensor, as illustrated in Fig. 1. The surfaces of the gold electrodes are modified using molecularly-imprinted polymers. Electrochemical methods were used to monitor the self-assembly of cysteine on the gold surface. The thiol-gold self-assembled layer causes impedance and mass change, which was characterised by EIS and acoustic-wave methods, respectively. Preliminary tests were performed on this sensor using cysteine in both phosphate buffer saline and human serum.

  • 20.
    Osikoya, Adeniyi
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Vanderbijlpark, South Africa.
    Parlak, Onur
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Murugan, N .Arul
    Stockholm, Sweden.
    Dikio, Ezekiel Dixon
    Vanderbijlpark, South Africa.
    Moloto, Harry
    Vanderbijlpark, South Africa.
    Uzun, Lokman
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Department of Chemistry, Hacettepe University, Ankara, Turkey.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. UCS, Tekidag AB, Linkoping,, Sweden; Vinoba Bhave Research Institute, Sirsa Road, Saidabad, Allahabad 221508, India.
    Acetylene-sourced CVD-synthesised catalytically active graphene for electrochemical biosensing.2017In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 89, p. 496-504Article in journal (Refereed)
    Abstract [en]

    In this study, we have demonstrated the use of a graphene sheet as a fundamental building block to obtain a highly ordered graphene-enzyme electrode for electrochemical biosensing. Firstly, thin graphene sheets were deposited on 1.00 mm thick copper sheet at 850 oC, via chemical vapour deposition (CVD), using acetylene (C2H2) as carbon source in an argon (Ar) and nitrogen (N2) atmosphere. An anionic surfactant was used to introduce electrostatic charges and increase wettability and hydrophilicity on the basal plane of the otherwise hydrophobic graphene, thereby facilitating the assembly of biomolecules on the graphene surface. The bioelectrocatalytic activity of the system was investigated by the assembly of glucose oxidase (GOx) on the surface of the graphene sheet by intermolecular attractive forces. The electrochemical sensing activity of the graphene-based system was explored as a model for bioelectrocatalysis. The bioelectrode exhibited a linear response to glucose concentration from 0.2 to 9.8 mM, with sensitivity of 0.087 µA/µM/cm2 and a detection limit of 0.12 µM (S/N=3). This work sets the stage for the use of acetylene-sourced graphene sheets as fundamental building blocks in the fabrication of electrochemical biosensors and other biocatalytic devices.

  • 21.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Ashaduzzaman, Md.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Dhaka, Bangladesh.
    Kollipara, Suresh B.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Faculty of Science & Engineering. Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. UCS, Tekidag AB, SE-58330 Linkoping, Sweden.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Switchable Bioelectrocatalysis Controlled by Dual Stimuli-Responsive Polymeric Interface2015In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, no 43, p. 23837-23847Article in journal (Refereed)
    Abstract [en]

    The engineering of bionanointerfaces using stimuli-responsive polymers offers a new dimension in the design of novel bioelectronic interfaces. The integration of electrode surfaces with stimuli-responsive molecular cues provides a direct control and ability to switch and tune physical and chemical properties of bioelectronic interfaces in various biodevices. Here, we report a dual-responsive biointerface employing a positively responding dual-switchable polymer, poly(NIPAAm-co-DEAEMA)-b-HEAAm, to control and regulate enzyme-based bioelectrocatalysis. The design interface exhibits reversible activation deactivation of bioelectrocatalytic reactions in response to change in temperature and in pH, which allows manipulation of biomolecular interactions to produce on/off switchable conditions. Using electrochemical measurements, we demonstrate that interfacial bioelectrochemical properties can be tuned over a modest range of temperature (i.e., 20-60 degrees C) and pH (i.e., pH 4-8) of the medium. The resulting dual-switchable interface may have important implications not only for the design of responsive biocatalysis and on-demand operation of biosensors, but also as an aid to elucidating electron-transport pathways and mechanisms in living organisms by mimicking the dynamic properties of complex biological environments and processes.

  • 22.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Beyazit, Selim
    CNRS Enzyme and Cell Engineering Laboratory, Université de Technologie de Compiègn Rue Roger Couttolenc, Compiègne, Cedex, France.
    Jafari, Mohammed J.
    Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
    Tse Sum Bui, Bernadette
    CNRS Enzyme and Cell Engineering Laboratory, Université de Technologie de Compiègn Rue Roger Couttolenc, Compiègne, Cedex, France.
    Haupt, Karsten
    CNRS Enzyme and Cell Engineering Laboratory, Université de Technologie de Compiègn Rue Roger Couttolenc, Compiègne, Cedex, France.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Tekidag AB, UCS, Linköping, Sweden.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Light-triggered switchable graphene-polymer hybrid bioelectronics2016In: Advanced Materials Interfaces, ISSN 2196-7350, Vol. 3, no 2, p. 1500353-1-1500353-7Article in journal (Refereed)
    Abstract [en]

    A light-switchable graphene interface to control and regulate electrobiocatalysis in a nanoconfined space is reported for the first time. The development of switchable and/or tunable interfaces on 2D nanosurfaces endowed with desirable functionalities, and incorporation of these interfaces into remote controlled biodevices, is a rapidly emerging area in bioelectronics.

  • 23.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Beyazit, Selim
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Jafari, Mohammed J.
    Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
    Tse Sum Bui, Bernadette
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Haupt, Karsten
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Tiwari, Ashutosh
    Tekidag AB, UCS, Mjärdevi Science Park, Linköping Sweden.
    Turner, Anthony P. F
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Light-triggered on/off-switchable graphene-based bioelectronicsManuscript (preprint) (Other academic)
  • 24.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Beyazit, Selim
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Tse Sum Bui, Bernadette
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Haupt, Karsten
    Sorbonne Universités, Université de Technologie de Compiègne, CNRS Laboratory for Enzyme and Cell Engineering, France.
    Turner, Anthony P. F
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Tekidag AB, UCS, Mjärdevi Science Park, Linköping Sweden.
    Programmable bioelectronics in a stimuli-encoded 3D grapheneManuscript (preprint) (Other academic)
  • 25.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Beyazit, Selim
    Compiègne Cedex, France.
    Tse Sum Bui, Bernadette
    Compiègne Cedex, France.
    Haupt, Karsten
    Compiègne Cedex, France.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Vinoba Bhave Research Institute, Sirsa Road, Saidabad, India .
    Programmable bioelectronics in a stimuli-encoded 3D graphene interfaces2016In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, p. 9976-9981Article in journal (Refereed)
    Abstract [en]

    The ability to program and mimic the dynamic microenvironment of living organisms is a crucial step towards the engineering of advanced bioelectronics. Here, we report for the first time a design for programmable bioelectronics, with ‘built-in’ switchable and tunable bio-catalytic performance that responds simultaneously to appropriate stimuli. The designed bio-electrodes comprise light and temperature responsive compartments, which allow the building of Boolean logic gates (i.e. “OR” and “AND”) based on enzymatic communications to deliver logic operations.

  • 26.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Mishra, Yogendra K.
    Functional Nanomaterials, Institute for Materials Science, Christian-Albrechts Universität zu Kiel, Kiel, Germany.
    Grigoriev, Anton
    Department of Physics and Astronomy, Uppsala University, Uppsala, Sweden.
    Mecklenburg, Matthias
    Institute of Polymers and Composites, Hamburg University of Technology, Hamburg, Germany.
    Schulte, Karl
    Institute of Polymers and Composites, Hamburg University of Technology, Hamburg, Germany.
    Ahuja, Rajeev
    Department of Physics and Astronomy, Uppsala University, Uppsala, Sweden.
    Adelung, Rainer
    Functional Nanomaterials, Institute for Materials Science, Christian-Albrechts Universität zu Kiel, Kiel, Germany.
    Turner, Anthony P. F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Tekidag AB, UCS, Mjärdevi Science Park, Linköping Sweden.
    Probing electrocatalytic properties of aerographite for the design of flexible bio-electrodeManuscript (preprint) (Other academic)
  • 27.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Mishra, Yogendra Kumar
    Functional Nanomaterials, Institute for Materials Science, Kiel University, Kiel, Germany.
    Grigoriev, Anton
    Department of Physics and Astronomy, Uppsala University, Uppsala, Sweden.
    Mecklenburg, Matthias
    e Institute of Polymers and Composites, Hamburg University of Technology, Hamburg, Germany.
    Luo, Wei
    d Department of Physics and Astronomy, Uppsala University, Uppsala, Sweden.
    Keene, Scott
    Department of Materials Science and Engineering, Stanford University, Stanford, USA.
    Salleo, Alberto
    Department of Materials Science and Engineering, Stanford University, Stanford, USA.
    Schulte, Karl
    e Institute of Polymers and Composites, Hamburg University of Technology, Hamburg, Germany.
    Ahuja, Rajeev
    d Department of Physics and Astronomy, Uppsala University, Uppsala, Sweden.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Hierarchical Aerographite Nano-Microtubular Tetrapodal Networks based Electrodes as Lightweight Supercapacitor.2017In: Nano Energy, ISSN 2211-2855, E-ISSN 2211-3282, Vol. 34, p. 570-577Article in journal (Refereed)
    Abstract [en]

    A great deal of interest has been paid to the application of carbon-based nano- and microstructured materials as electrodes due to their relatively low-cost production, abundance, large surface area, high chemical stability, wide operating temperature range, and ease of processing including many more excellent features. The nanostructured carbon materials usually offer various micro-textures due to their varying degrees of graphitisation, a rich variety in terms of dimensionality as well as morphologies, extremely large surface accessibility and high electrical conductivity, etc. The possibilities of activating them by chemical and physical methods allow these materials to be produced with further higher surface area and controlled distribution of pores from nanoscale upto macroscopic dimensions, which actually play the most crucial role towards construction of the efficient electrode/electrolyte interfaces for capacitive processes in energy storage applications. Development of new carbon materials with extremely high surface areas could exhibit significant potential in this context and motivated by this in present work, we report for the first time the utilization of ultralight and extremely porous nano-microtubular Aerographite  tetrapodal network as a functional interface to probe the electrochemical properties for capacitive energy storage. A simple and robust electrode fabrication strategy based on surface functionalized Aerographite with optimum porosity leads to significantly high specific capacitance (640 F/g) with high energy (14.2 Wh/kg) and power densities (9.67x103 W/kg) which has been discussed in detail.

  • 28.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Seshadri, Preeethi
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Structuring of nanoelectrode array of Au nanoparticle on two-dimensional WS2 interface for electrochemical biosensing2014In: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper (Other academic)
    Abstract [en]

    The reduction in size of an electrode improves the mass transport of molecules to the electrode surface because of the contribution of radial diffusion. Thus, nanoelectrodes are particularly well suited for electroanalytical applications [1]. However, the construction of nanoelectrodes generally involve specialised equipment that is relatively complex to fabricate and not suitable for mass production. However nanostructuring of macroelectrode surface by metallic nanoparticles have been recently used in a variety of biosensing applications due to their enhanced surface area, precise biomolecule-electrode connections. For electrochemical sensing, conductive nanostructures immobilised on electrodes enhance electrocatalytic behaviour due the quantum confinement and exhibit unique features including favourable Faradic-to-capacitive current ratios, higher current densities and faster mass transport by convergent diffusion than their larger micro/macro electrode counterparts. In order to increase biosensor current output to measurable levels, large arrays of nanostructures have been immobilised on electrode surface [2]. The nanoelectrode arrays biosensors have been fabricated by various nanostructures such as nanowires, nanotubes and nanoparticles, have demonstrated promising results, displaying high sensitivity and fast response time.

           In this study, we present a new nanostructured biosensor to address many limitations that nanoelectrode array biosensors currently face. Here, we used WS2-Au nanoparticle self-assembled structures as an interface element for electrochemical sensing of H2O2. The combination of zero-dimension nanoparticles on a two-dimensional support that is arrayed in the third dimension creates a biosensor platform with exceptional characteristics. The versatility of the biosensor platform was demonstrated by altering biosensor performance with a sensitivity (11.64 µA/µM/cm2), detection limit (0.085 µM), and linear sensing range (0.05-12.0 mM). This promising approach provides a novel methodology for structuring of Au nanoarray on two-dimensional surface and furnishes the basis for fabrication of flexible ultra-sensitive and efficient electrochemical biosensors.

     

  • 29.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Seshadri, Prethi
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Lundström, Ingemar
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony P.F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Two-dimensional gold-tungsten disulphide bio-interface for high-throughput electrocatalytic nano-bioreactors2014In: Advanced Materials Interfaces, ISSN 2196-7350, Vol. 1, no 6, p. 1400136-Article in journal (Refereed)
    Abstract [en]

    A high-throughput electrocatalytic nano-bioreactor on tungsten disulphide nanosheets is demonstrated for the first time. The fundamental goal of this research is to develop a higher surface area, resulting in a greater enzyme loading and thereby increasing bio-catalytic activity within a nano-confined volume. As a result, the nanobio-system is capable of highly specific recognition of target bioanalytes, therefore, showing significant potentials in a range of bioreactor applications.

  • 30.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Electro-catalytic nanoparticle-polymer fibre networks for efficient bioenergy devices.2015In: 1st International Conference on Green Chemistry and Sustainable Technologies, 2015Conference paper (Refereed)
  • 31.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Switchable Bioelectronics on a Graphene Interface2015In: 2nd International Biosensor Congress, 2015Conference paper (Other academic)
  • 32.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Switchable bioelectronics on graphene interface.2015In: Biosensing and Nanomedicine-VIII at SPIE Optics and Photonics, San Diego, CA, 9-13 August 2015., 2015Conference paper (Refereed)
    Abstract [en]

    Smart and flexible bioelectronics on graphene have emerged as a new frontier in the field of biosensors and bioelectronics. Graphene has begun to be seen as an ideal signal transducer and a promising alternative for the production of low-cost bioelectronic devices.1-2 However, biological systems used in these devices suffer from a lack of control and regulation. There is an obvious need to develop “switchable” and “smart” interfaces for both fundamental and applied studies. Here, we report the fabrication of a stimuli-responsive graphene interface, which is used to regulate biomolecular reactions.

    The present study aims to address the design and development of a novel auto-switchable graphene bio-interface that is capable of positively responding, by creating smart nanoarchitectures. The smart bio-interface consists of a two-dimensional graphene donor and a polymeric receptor, which are rationally assembled together based in a stoichiometric donor-receptor interaction. By changing the external conditions such as temperature, light and pH of the medium, we acheived control of the biochemical interactions. In the negative mode, access of an associated enzyme to its substrate is largely restricted, resulting in a decrease in the diffusion of reactants and the consequent activity of the system. In contrast, the biosubstrate could freely access the enzyme facilitating bioelectrocatalysis in a positive response. More importantly, this provides the first example of responsive bioelectronics being achieved on a two-dimensional graphene interface by controlling the various external stimuli in an on/off-switchable model.

    Using electrochemical techniques, we demonstrated that interfacial bio-electrochemical properties can be tuned by modest changes in conditions. Such an ability to independently regulate the behaviour of the interface has important implications for the design of novel bioreactors, biofuel cells and biosensors with inbuilt self-control features.

    Reference:

    [1] O. Parlak, A. P. F. Turner, A. Tiwari, Advanced Materials, 3 (2014), 482.

    [2] O. Parlak, A. Tiwari, A. P. F. Turner, A. Tiwari, Biosensors and Bioelectronics 49         (2013), 53.

  • 33.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Atul
    Hawaii Corrosion Laboratory, Department of Mechanical Engineering, University of Hawaii at Manoa, 96822 Hawaii, USA.
    Turner, Anthony P. F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Template-Directed Hierarchical Self-Assembly of Graphene Based Hybrid Structure for Electrochemical Biosensing2013In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 49, p. 53-62Article in journal (Refereed)
    Abstract [en]

    A template-directed self-assembly approach, using functionalised graphene as a fundamental building block to obtain a hierarchically ordered graphene-enzyme-nanoparticle bioelectrode for electrochemical biosensing, is reported. An anionic surfactant was used to prepare a responsive, functional interface and direct the assembly on the surface of the graphene template. The surfactant molecules altered the electrostatic charges of graphene, thereby providing a convenient template-directed assembly approach to a free-standing planar sheet of sp(2) carbons. Cholesterol oxidase and cholesterol esterase were assembled on the surface of graphene by intermolecular attractive forces while gold nanoparticles are incorporated into the hetero-assembly to enhance the electro-bio-catalytic activity. Hydrogen peroxide and cholesterol were used as two representative analytes to demonstrate the electrochemical sensing performance of the graphene-based hybrid structure. The bioelectrode exhibited a linear response to H2O2 from 0.01 to 14 mM, with a detection limit of 25 nM (S/N=3). The amperometric response with cholesterol had a linear range from 0.05 to 0.35 mM, sensitivity of 3.14 mu A/mu M/cm(2) and a detection limit of 0.05 mu M. The apparent Michaelis-Menten constant (K-m(app)) was calculated to be 1.22 mM. This promising approach provides a novel methodology for template-directed bio-self-assembly over planar sp(2) carbons of a graphene sheet and furnishes the basis for fabrication of ultra-sensitive and efficient electrochemical biosensors.

  • 34.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Turner, Anthony P. F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Tekidag AB, UCS, Mjärdevi Science Park, Linköping Sweden.
    pH-induced on/off-switchable graphene bioelectronics2015In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 3, no 37, p. 7434-7439Article in journal (Refereed)
    Abstract [en]

    Switchable interfaces can deliver functionally reversible reactivity with their corresponding analytes, which thus allows one to positively respond the activity of biological elements, including enzymes and other biomolecules, through an encoded stimulus. We have realised this by the design of stimuli-responsive graphene interfaces for pH-encoded operation of bioelectronics. In this study, we have demonstrated stimuli-responsive graphene interfaces for pH-encoded operation of bioelectronics. The resulting switchable interfaces are capable of highly specific, on-demand operation of biosensors, which has significant potential in a wide range of analytical applications.

  • 35.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    On/off-switchable zipper-like bioelectronics on a graphene interface.2014In: Advanced Materials, ISSN 0935-9648, E-ISSN 1521-4095, Vol. 26, no 3, p. 482-486Article in journal (Refereed)
    Abstract [en]

    An on/off-switchable graphene based zipper-like interface is architectured for efficient bioelectrocatalysis. The graphene interface transduces a temperature input signal into structural changes of the membrane, resulting in the amplification of electrochemical signals and their transformation into the gated transport of molecules through the membrane.

  • 36.
    Parlak, Onur
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Switchable bioelectronics on a graphene interface2014In: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper (Other academic)
    Abstract [en]

    Smart and flexible bioelectronics on graphene have emerged as a new frontier in the field of biosensors and bioelectronics [1]. Graphene has begun to be seen as an ideal signal transducer and a promising alternative for the production of low-cost bioelectronic devices. However, biological systems used in these devices suffer from a lack of control and regulation. There is an obvious need to develop “switchable” and “smart” interfaces for both fundamental and applied studies [2]. Here, we report for the first time the fabrication of an on/off-switchable graphene interface, which is used to regulate biomolecular reactions.

        The present study aims to address the design and development of a novel auto-switchable graphene bio-interface that is capable of positively responding, by creating unique “zipper” nanoarchitectures. The zipper consists of a two-dimensional graphene donor and a polymeric receptor, which are rationally assembled together based in a stoichiometric donor-receptor interaction. Preferably, at a relatively low temperature (20 oC) the active donor-receptor interaction (hydrogen bonding) creates a coalescence of the graphene interface, thereby causing considerable shrinkage in the donor-to-receptor interface. Thus access of an associated enzyme to its substrate is largely restricted, resulting in a decrease in the diffusion of reactants and the consequent activity of the system. In contrast, at a comparatively high temperature (40 oC) the donor-receptor interaction was subverted. As a result, the biosubstrate could freely access the enzyme facilitating bioelectrocatalysis. More importantly, this provides the first example of responsive bioelectronics being achieved on a two-dimensional graphene interface by controlling the external temperature as an on/off-switchable model.

         Using electrochemical techniques, we demonstrated that interfacial bio-electrochemical properties can be tuned by modest changes in temperature. Such an ability to independently regulate the behaviour of the interface has important implications for the design of novel bioreactors, biofuel cells and biosensors with inbuilt self-control features.

     

  • 37.
    Patra, Hirak
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Imani, Roghayeh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Iglic, Ales
    Biophysics Laboratory, Faculty of Electrical Engineering, University of Ljubljana, Slovenia.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Novel anti-neoplastic approach for photodynamic theranostics by biocompatible TiO2 popcorn nanostructure for a high-throughput flash ROS generator2014In: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper (Refereed)
    Abstract [en]

    Reactive oxygen species (ROS) are important secondary messengers in the intracellular signaling system for regulating redox homeostasis in normal cells. Compared to normal cells, cancer cells have increased ROS levels due to a faster metabolic rate. We have used this discriminating overproduction of ROS levels in cancer cells  as a target for a photodynamic anti-neoplastic theranostic approach using mesoporous TiO2 microbeads with a popcorn nanostructure. We have created a novel flash ROS generator  using a two-step procedure consisting of sol-gel and solvothermal processes to obtain mesoporous TiO2 microbeads with high photocatalytic efficiency. A photon-induced comparative study has been carried out for the ROS generation ability using TiO2 nanoparticles and mesoporous TiO2 microbeads.  We have shown that in under otherwise identical conditions the extent of photocatalytical ROS generated by mesoporous TiO2 microbeads is more than twice that produced by TiO2 nanoparticles. In vitro in the absence of irradiation, the mesoporous TiO2 microbeads are exceptionally biocompatible, allowing almost ~100% cellular survival rate even at a dose of 100 µg/mL. In contrast, commercial nanoparticles showed concentration dependent cytotoxicity of nearly 15% within 24h in the absence of any irradiation. Upon photo activation, the mesoporous TiO2 microbead structures delivered their potential anticancer effect by interfering with the mitochondrial activity by producing ROS in the intracellular environment and thus reducing the survival rate of cells by more than 30% in comparison with commercial nanoparticles, where only an increase of 5% in cell death was observed. Thus we have developed a smart on/off switchable photodynamic anti-neoplastic theranostic approach that can be combined with specific cell recognition elements for future cancer management.

  • 38.
    Patra, Hirak Kumar
    et al.
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
    Imani, Roghayeh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Univ,of Ljubljana, Slovenia; University of Ljubljana, Slovenia.
    Jangamreddy, Jaganmohan
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
    Pazoki, Meysam
    Uppsala University, Sweden.
    Iglic, Ales
    University of Ljubljana, Slovenia.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering. Tekidag AB, SE-58330 Linkoping, Sweden.
    On/off-switchable anti-neoplastic nanoarchitecture2015In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, no 14571, p. 1-9Article in journal (Refereed)
    Abstract [en]

    Throughout the world, there are increasing demands for alternate approaches to advanced cancer therapeutics. Numerous potentially chemotherapeutic compounds are developed every year for clinical trial and some of them are considered as potential drug candidates. Nanotechnology-based approaches have accelerated the discovery process, but the key challenge still remains to develop therapeutically viable and physiologically safe materials suitable for cancer therapy. Here, we report a high turnover, on/off-switchable functionally popping reactive oxygen species (ROS) generator using a smart mesoporous titanium dioxide popcorn (TiO2 Pops) nanoarchitecture. The resulting TiO2 Pops, unlike TiO2 nanoparticles (TiO2 NPs), are exceptionally biocompatible with normal cells. Under identical conditions, TiO2 Pops show very high photocatalytic activity compared to TiO2 NPs. Upon on/off-switchable photo activation, the TiO2 Pops can trigger the generation of high-turnover flash ROS and can deliver their potential anticancer effect by enhancing the intracellular ROS level until it crosses the threshold to open the death gate, thus reducing the survival of cancer cells by at least six times in comparison with TiO2 NPs without affecting the normal cells.

  • 39.
    Patra, Hirak Kumar
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Khaliq, Nisar Ul
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Romu, Thobias
    Linköping University, Center for Medical Image Science and Visualization (CMIV). Linköping University, Department of Biomedical Engineering, Medical Informatics. Linköping University, The Institute of Technology.
    Wiechec, Emilia
    Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology.
    Borga, Magnus
    Linköping University, Center for Medical Image Science and Visualization (CMIV). Linköping University, Department of Biomedical Engineering, Medical Informatics. Linköping University, The Institute of Technology.
    Turner, Anthony P. F.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    MRI-Visual Order–Disorder Micellar Nanostructures for Smart Cancer Theranostics2014In: Advanced Healthcare Materials, ISSN 2192-2640, Vol. 3, no 4, p. 526-535Article in journal (Refereed)
    Abstract [en]

    The development of MRI-visual order–disorder structures for cancer nanomedicine explores a pH-triggered mechanism for theragnosis of tumor hallmark functions. Superparamagnetic iron oxide nanoparticles (SPIONs) stabilized with amphiphilic poly(styrene)-b-poly(acrylic acid)-doxorubicin with folic acid (FA) surfacing are employed as a multi-functional approach to specifically target, diagnose, and deliver drugs via a single nanoscopic platform for cancer therapy. The functional aspects of the micellar nanocomposite is investigated in vitro using human breast SkBr3 and colon cancer HCT116 cell lines for the delivery, release, localization, and anticancer activity of the drug. For the first time, concentration-dependent T2-weighted MRI contrast for a monolayer of clustered cancer cells is shown. The pH tunable order–disorder transition of the core–shell structure induces the relative changes in MRI contrast. The outcomes elucidate the potential of this material for smart cancer theranostics by delivering non-invasive real-time diagnosis, targeted therapy, and monitoring the course and response of the action before, during, and after the treatment regimen.

  • 40.
    Patra, Hirak
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Smart inflammation sensitive self-reporting theragnosis2014In: FEBS-EMBO 2014 Congress, 2014Conference paper (Other academic)
    Abstract [en]

    We have designed and develop a novel class of nanocomposites for inflammation based hallmark functions using biocompatible metallic nano-objects (SPION, nanorod) assembled with a pH sensitive amphiphilic azide terminated block polymer, polystyrene-b-poly (acrylic acid) and temperature-responsive polymer Poly (N-isopropylacrylamide) (PNIPAAm) in a single nanoscopic platform. The nano-architecture is a uniform core-shell micellar assembly of polymer around the biocompatible metallic core. Doxorubicin and methotrexate are loaded within the architecture as the model therapeutic module. The drugs are linked through pH and enzyme sensitive bonds. The complete nano-architecture and linkages are characterized by electron microscopy, NMR and Photon Correlation Spectroscopy. The drug release response has been optimized with different cell line in vitro. The model suggest that change/increase in temperature, reduction of pH and the redox enzymatic activities are increased at the localized inflammatory sites, can be addressed by the developed module and the drug will be released at the inflammation sites only due to their specific linkage to the module. Again we have explored order–disorder micellar structures dependent T1 & T2 MRI properties of the module. This results indicate that the fabricated module can also be useful not only probing the inflammation site non invasively through MRI but also will give us idea on the extent of release of drugs at the inflammation sites. The outcomes of these results elucidate the potential of this fabricated nano-architecture for smart theranostics through physicochemical and microenvironment feature based drug delivery, site-specific therapy, real-time probing and non-invasive monitoring of the drug action course for personalized therapy.

     

  • 41.
    Rai, Radheshyam
    et al.
    Aveiro University, Portugal.
    Sharma, Seema
    Magadh University, India.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Synthesis and Characterization of Bi, Fe, Al and Sb- Modified PLZT2012In: Synthesis, Characterization and Application of Smart Materials / [ed] Radheshyam Rai, USA: Nova Science Publishers, Inc., 2012, p. 31-98Chapter in book (Other academic)
    Abstract [en]

    Smart materials, one of the more focused points in materials research, deal primarily with the chemistry, physics and applications of materials in the real world because it induces conformational changes in complex structures and properties which are useful for the control of them. The thrust area of these types of materials are the combination of functional properties like thermal, electric, magnetic, superconducting and optical, which have led to the development of a wide range of new technological devices. These types of materials have been found to be very useful and interesting for various solid state devices. This book examines research developments of smart materials, including processing, properties and applications, which include device materials and environmentally friendly materials. (Imprint: Nova)

  • 42.
    Rai, Radheshyam
    et al.
    Aveiro University, Portugal.
    Sharma, Seema
    Magadh University, India.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Singh, R. P.
    University of Allahabad, India.
    Experimental Techniques: An Introductory Overview2012In: Synthesis, Characterization and Application of Smart Materials / [ed] Radheshyam Rai, USA: Nova Science Publishers, Inc., 2012, p. 15-30Chapter in book (Other academic)
    Abstract [en]

    Smart materials, one of the more focused points in materials research, deal primarily with the chemistry, physics and applications of materials in the real world because it induces conformational changes in complex structures and properties which are useful for the control of them. The thrust area of these types of materials are the combination of functional properties like thermal, electric, magnetic, superconducting and optical, which have led to the development of a wide range of new technological devices. These types of materials have been found to be very useful and interesting for various solid state devices. This book examines research developments of smart materials, including processing, properties and applications, which include device materials and environmentally friendly materials. (Imprint: Nova)

  • 43.
    Ramalingam, Murugan
    et al.
    Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg 67085, France.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Polymeric Nanofibers and their Applications in Sensors2012In: Intelligent Nanomaterials: processes, properties, and applications / [ed] Ashutosh Tiwari, Ajay Kumar Mishra, Hisatoshi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 801-823Chapter in book (Other academic)
    Abstract [en]

    The last three decades have seen extraordinary advances in the generation of new materials based on both fundamental elements and composites, driven by advances in synthetic chemistry and often drawing inspiration from nature. The concept of an intelligent material envisions additional functionality built into the molecular structure, such that a desirable response occurs under defined conditions.

  • 44.
    Ramalingam, Murugan
    et al.
    Institut National de la Santé et de la Recherche Médicale U977, Faculté de Chirurgie Dentaire, Université de Strasbourg (UdS), France.
    Tiwari, AshutoshLinköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.Ramakrishna, SeeramHEM Labs at the National University of Singapore.Kobayashi, HisatoshiNational Institute for Materials Science, Tsukuba, Japan.
    Integrated Biomaterials for Biomedical Technology2012Collection (editor) (Other academic)
    Abstract [en]

    This cutting edge book provides all the important aspects dealing with the basic science involved in materials in biomedical technology, especially structure and properties, techniques and technological innovations in material processing and characterizations, as well as the applications. The volume consists of 12 chapters written by acknowledged experts of the biomaterials field and covers a wide range of topics and applications.

  • 45.
    Sanjay, Sharda Sunaram
    et al.
    Chemistry Department, Ewing Christian College Allahabad, Allahabad-211002, India.
    Singh, Ravindra P.
    Nano technology Application Centre, University of Allahabad, Allahabad 211002, India.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Pandey, Avinash C.
    Nano technology Application Centre, University of Allahabad, Allahabad-211002, India.
    Mode of Growth Mechanism of Nanocrystal Using Biomolecules2012In: Intelligent Nanomaterials: processes, properties, and applications / [ed] Ashutosh Tiwari, Ajay Kumar Mishra, Hisatoshi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 625-648Chapter in book (Other academic)
    Abstract [en]

    The last three decades have seen extraordinary advances in the generation of new materials based on both fundamental elements and composites, driven by advances in synthetic chemistry and often drawing inspiration from nature. The concept of an intelligent material envisions additional functionality built into the molecular structure, such that a desirable response occurs under defined conditions.

  • 46.
    Sharma, Deepali
    et al.
    Durban University of Technology, South Africa.
    Ashaduzzaman, Md.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Golabi, Mohsen
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Shriwastav, Amritanshu
    Indian Institute Technology, India.
    Bisetty, Krishna
    Durban University of Technology, South Africa.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology. Kanpur 208016, Uttar Pradesh, India..
    Studies on Bacterial Proteins Corona Interaction with Saponin Imprinted ZnO Nanohoneycombs and Their Toxic Responses2015In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, no 43, p. 23848-23856Article in journal (Refereed)
    Abstract [en]

    Molecular imprinting generates robust, efficient, and highly mesoporous surfaces for biointeractions. Mechanistic interfacial interaction between the surface of core substrate and protein corona is crucial to understand the substantial microbial toxic responses at a nanoscale. In this study, we have focused on the mechanistic interactions between synthesized saponin imprinted zinc oxide nanohoneycombs (SIZnO NHs), average size 80-125 nm, surface area 20.27 m(2)/g, average pore density 0.23 pore/nm and number-average pore size 3.74 nm and proteins corona of bacteria. The produced SIZnO NHs as potential antifungal and antibacterial agents have been studied on Sclerotium rolfsii (S. rolfsii), Pythium debarynum (P. debarynum) and Escherichia coil (E. coli), Staphylococcus aureus (S. aureus), respectively. SIZnO NHs exhibited the highest antibacterial (similar to 50%) and antifungal (similar to 40%) activity against Gram-negative bacteria (E. coil) and fungus (P. debarynum), respectively at concentration of 0.1 mol. Scanning electron spectroscopy (SEM) observation showed that the ZnO NHs ruptured the cell wall of bacteria and internalized into the cell. The molecular docking studies were carried out using binding proteins present in the gram negative bacteria (lipopolysaccharide and lipocalin Blc) and gram positive bacteria (Staphylococcal Protein A, SpA). It was envisaged that the proteins present in the bacterial cell wall were found to interact and adsorb on the surface of SIZnO NHs thereby blocking the active sites of the proteins used for cell wall synthesis. The binding affinity and interaction energies were higher in the case of binding proteins present in gram negative bacteria as compared to that of gram positive bacteria. In addition, a kinetic mathematical model (KMM) was developed in MATLAB to predict the internalization in the bacterial cellular uptake of the ZnO NHs for better understanding of their controlled toxicity. The results obtained from KMM exhibited a good agreement with the experimental data. Exploration of mechanistic interactions, as well as the formation of bioconjugate of proteins and ZnO NHs would play a key role to interpret more complex biological systems in nature.

  • 47.
    Sharma, Yashpal
    et al.
    National Institute Mat Science, Japan GJ University of Science and Technology, India .
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Hattori, Shinya
    National Institute Mat Science, Japan .
    Terada, Dohiko
    National Institute Mat Science, Japan .
    K Sharma, Ashok
    DCR University of Science and Technology, India .
    Ramalingam, Murugan
    University of Strasbourg, France Tohoku University, Japan .
    Kobayashi, Hisatoshi
    National Institute Mat Science, Japan JST CREST, Japan .
    Fabrication of conducting electrospun nanofibers scaffold for three-dimensional cells culture2012In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 51, no 4, p. 627-631Article in journal (Refereed)
    Abstract [en]

    Electrospinning is a versatile method to fabricate nanofibers of a range of polymeric and composite materials suitable as scaffolds for tissue engineering applications. In this study, we report the fabrication and characterization of polyaniline-carbon nanotube/poly(N-isopropyl acrylamide-co-methacrylic acid) (PANI-CNT/PNIPAm-co-MAA) composite nanofibers and PNIPAm-co-MAA nanofibers suitable as a three-dimensional (3D) conducting smart tissue scaffold using electrospinning. The chemical structure of the resulting nanofibers was characterized with FUR and H-1 NMR spectroscopy. The surface morphology and average diameter of the nanofibers were observed by SEM. Cellular response of the nanofibers was studied with mice L929 fibroblasts. Cell viability was checked on 7th day of cell culture by double staining the cells with calcein-AM and PI dye. PANI-CNT/PNIPAm-co-MAA composite nanofibers were shown the highest cell growth and cell viability as compared to PNIPAm-co-MAA nanofibers. Cell viability in the composite nanofibers was obtained in order of 98% that indicates the composite nanofibers provide a better environment as a 3D scaffold for the cell proliferation and attachment suitable for tissue engineering applications.

  • 48.
    Sharma, Yashpal
    et al.
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Kobayashi, Hisatoshi
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
    Conducting Polymer Composites for Tissue Engineering Scaffolds2012In: Biomedical Materials and Diagnostic Devices / [ed] Ashutosh Tiwari, Murugan Ramalingam, Hisashi Kobayashi, Anthony P. F. Turner, USA: John Wiley & Sons, 2012, p. 597-510Chapter in book (Other academic)
    Abstract [en]

    "The functional materials with the most promising outlook have the ability to precisely adjust the biological phenomenon in a controlled mode. Engineering of advanced bio- materials has found striking applications in used for biomedical and diagnostic device applications, such as cell separation, stem-cell, drug delivery, hyperthermia, automated DNA extraction, gene targeting, resonance imaging, biosensors, tissue engineering and organ regeneration"--Provided by publisher. 

  • 49.
    Sharma, Yashpal
    et al.
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Kobayashi, Hisatoshi
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan.
    Electrospun Nanofiberfor Three Dimensional Cell Culture2013In: Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering / [ed] Ashutosh Tiwari, Atul Tiwari, USA: John Wiley & Sons, 2013, p. 417-434Chapter in book (Other academic)
    Abstract [en]

    "Nanoscopic therapeutic systems incorporate therapeutic agents, molecular targeting and diagnostic imaging capabilities and are emerging as the next generation of multifarious nanomedicine to improve the therapeutic outcome including chemo and translational therapy. This reference work is one of the first to cover Nanotheragnostics which is the new edge of nanomedicine combining both diagnostic and therapeutic elements at nano level. This large multidisciplinary reference work is has four main parts: Biocompatible Nanomaterials; Nanomedicine: Drug Gene and Cell Delivery; Multi-functional Nanocarrier: Diagnosis, Imaging and Treatment; Tissue Engineering/Regenerative Medicine"--

  • 50.
    Shukla, S. K.
    et al.
    Bhaskaracharya College of Applied Sciences, University of Delhi, Delhi 110075, India.
    Bharadvaja, Anand
    Bhaskaracharya College of Applied Sciences, University of Delhi, Delhi 110075, India.
    Parashar, G. K.
    Swami Shradhanand College, University of Delhi, Delhi 110036, India.
    Mishra, A. P.
    Department of Science and Technology, Technology Bhavan, Delhi 110061, India.
    Dubey, G. C.
    Institute of Defense Scientists and Technologists, S. K. Mazumdar Road, Delhi110054, India.
    Tiwari, Ashutosh
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Fabrication of ultra-sensitive optical fiber based humidity sensor using TiO2 thin film2012In: Advanced Materials Letters, ISSN 0976-3961, Vol. 3, no 5, p. 365-370Article in journal (Refereed)
    Abstract [en]

    Thin films of titanium dioxide in anatase form have been prepared using isobutyl titanate as precursor. The resulting TiO2 was coated on an U-shaped pyrex glass rod to sense the humidity of a controlled humid environment using optical fiber approach. The humidity sensing characteristics and the sensing mechanism have been investigated by measuring the output power of the sensor at different humidity. The developed humidity sensor was responded in the humidity ranging from 10 to 95% of relative humidity and exhibited the sensitivity of 0.78, response time 36s and recovery time 73s.

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