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  • 1.
    Jaaskelainen, Anne J.
    et al.
    Helsinki University of Central Hospital, Finland; University of Helsinki, Finland.
    Kallio-Kokko, Hannimari
    Helsinki University of Central Hospital, Finland; University of Helsinki, Finland.
    Ozkul, Aykut
    Ankara University, Turkey.
    Bodur, Hurrem
    Ankara Numune Training and Research Hospital, Turkey.
    Korukruoglu, Gulay
    Public Health Institute Turkey, Turkey.
    Mousavi, Mehrdad
    Swedish Institute Communicable Disease Control, Sweden.
    Pranav, Patel
    Robert Koch Institute, Germany.
    Vaheri, Antti
    University of Helsinki, Finland.
    Mirazimi, Ali
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Swedish Institute Communicable Disease Control, Sweden; Karolinska Institute, Sweden.
    Vapalahti, Olli
    Helsinki University of Central Hospital, Finland; University of Helsinki, Finland; University of Helsinki, Finland.
    Development and Evaluation of a Real-Time RT-qPCR for Detection of Crimean-Congo Hemorrhagic Fever Virus Representing Different Genotypes2014In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 14, no 12, p. 870-872Article in journal (Refereed)
    Abstract [en]

    Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic disease caused by a nairovirus belonging to family Bunyaviridae. The CCHF virus (CCHFV) can be transmitted to humans by Hyalomma ticks as well as by direct contact with infected body fluids or tissues from viremic livestock or humans. Our aim was to set up a fast RT-qPCR for detection of the different CCHFV genotypes in clinical samples, including an inactivation step to make the sample handling possible in lower biosafety levels (BSL) than BSL-4. This method was evaluated against commercial reference assays and international External Quality Assessment (EQA) samples. The analytical limit of detection for the developed CCHFV-S RT-qPCR was 11 CCHFV genomes per reaction. After exclusion of four dubious samples, we studied 38 CCHFV-positive samples (using reference tests) of which 38 were found positive by CCHFV-S RT-qPCR, suggesting a sensitivity of 100%. CCHFV-S RT q-PCR detected all eight different CCHFV strains representing five different CCHFV genotypes. In conclusion, the CCHFV-S RT-qPCR described in this study was evaluated using various sources of CCHFV samples and shown to be an accurate tool to detect human CCHFV infection caused by different genotypes of the virus.

  • 2.
    Karlberg, Helen
    et al.
    Public Health Agency Sweden, Sweden; Karolinska Institute, Sweden.
    Sharifi-Mood, Batool
    Zahedan University of Medical Science, Iran.
    Mousavi-Jazi, Mehrdad
    Public Health Agency Sweden, Sweden.
    Dilcher, Meik
    University of Medical Centre Gottingen, Germany.
    Lindegren, Gunnel
    Public Health Agency Sweden, Sweden.
    Mardani, Masoud
    Shahid Beheshti University of Medical Science, Iran.
    Bereskly, Sandor
    Public Health Agency Sweden, Sweden.
    Weidmann, Manfred
    University of Stirling, Scotland.
    Mirazimi, Ali
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Public Health Agency Sweden, Sweden; Karolinska Institute, Sweden; National Vet Institute, Sweden.
    Molecular and Serological Findings in Suspected Patients With Crimean-Congo Hemorrhagic Fever Virus in Iran2015In: Journal of Medical Virology, ISSN 0146-6615, E-ISSN 1096-9071, Vol. 87, no 4, p. 686-693Article in journal (Refereed)
    Abstract [en]

    Crimean-Congo hemorrhagic fever (CCHF) is an arthropod-borne disease of humans associated with a severe clinical picture, including hemorrhagic syndrome and a high mortality rate. CCHF virus is widely distributed throughout large areas of the world. To characterize the serological status in CCHF patients, paired clinical samples were collected from suspected CCHF patients and analyzed by microbiological and other laboratory analyses with the aim of: determining the presence of neutralizing antibodies against CCHF virus; investigating the cross-reactivity of these neutralizing antibodies against virus isolated from the same outbreak and against other available laboratory strain; and studying the relationship between the isolated virus with other virus by whole genome sequencing. Patients at Boo-Ali Hospital, Zahedan, Iran, with clinical symptoms ranging from mild to severe hemorrhagic fever were included in the study. Two serum samples were taken from each patient, the first as soon as the patient matched the criteria for CCHF notification and the second when the patient was discharged from hospital (2 weeks later). Commercial and in-house assays revealed a positive IgM signal in acute serum samples from six patients. A novel finding was that CCHF patients develop neutralizing antibodies soon after infection. Interestingly these antibodies were able to neutralize other CCHF virus strains too. The complete sequence of the Zahedan 2007 isolate, including the hitherto unknown first L-segment sequence, was identified using an original clinical sample from one patient with confirmed CCHF infection.

  • 3.
    Molinas, Andrea
    et al.
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Mirazimi, Ali
    Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden.
    Holm, Angelika
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Loitto, Vesa M.
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Magnusson, Karl-Eric
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Vikström, Elena
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Protective role of host aquaporin 6 against Hazara virus, a model for Crimean–Congo hemorrhagic fever virus infection2016In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 363, no 8, article id fnw058Article in journal (Refereed)
    Abstract [en]

    Crimean–Congo hemorrhagic fever virus (CCHFV) is an arthropod-borne pathogen that causes infectious disease with severe hemorrhagic manifestations in vascular system in humans. The proper function of the cells in the vascular system is critically regulated by aquaporins (AQP), water channels that facilitate fluxes of water and small solutes across membranes. With Hazara virus as a model for CCHFV, we investigated the effects of viruses on AQP6 and the impact of AQP6 on virus infectivity in host cells, using transiently expressed GFP-AQP6 cells, immunofluorescent assay for virus detection, epifluorescent imaging of living cells and confocal microscopy. In GFP-AQP6 expressing cells, Hazara virus reduced both the cellular and perinuclear AQP6 distribution and changed the cell area. Infection of human cell with CCHFV strain IbAR 10200 downregulated AQP6 expression at mRNA level. Interestingly, the overexpression of AQP6 in host cells decreased the infectivity of Hazara virus, speaking for a protective role of AQP6. We suggest the possibility for AQP6 being a novel player in the virus–host interactions, which may lead to less severe outcomes of an infection.

  • 4.
    Papa, Anna
    et al.
    Aristotle University of Thessaloniki, Greece.
    Mirazimi, Ali
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Swedish Institute Communicable Disease Control, Sweden; National Vet Institute, Sweden.
    Koksal, Iftihar
    Karadeniz Technical University, Turkey.
    Estrada-Pena, Augustin
    University of Zaragoza, Spain.
    Feldmann, Heinz
    NIAID, MT USA.
    Recent advances in research on Crimean-Congo hemorrhagic fever2015In: Journal of Clinical Virology, ISSN 1386-6532, E-ISSN 1873-5967, Vol. 64, p. 137-143Article in journal (Refereed)
    Abstract [en]

    Crimean-Congo hemorrhagic fever (CCHF) is an expanding tick-borne hemorrhagic disease with increasing human and animal health impact. Immense knowledge was gained over the past 10 years mainly due to advances in molecular biology, but also driven by an increased global interest in CCHFV as an emerging/re-emerging zoonotic pathogen. In the present article, we discuss the advances in research with focus on CCHF ecology, epidemiology, pathogenesis, diagnostics, prophylaxis and treatment. Despite tremendous achievements, future activities have to concentrate on the development of vaccines and antivirals/therapeutics to combat CCHF. Vector studies need to continue for better public and animal health preparedness and response. We conclude with a roadmap for future research priorities. (C) 2014 Elsevier B.V. All rights reserved.

  • 5.
    Papa, Anna
    et al.
    Aristotle University of Thessaloniki, Greece .
    Sidira, Persefoni
    Aristotle University of Thessaloniki, Greece .
    Larichev, Victor
    DI Ivanovskii Institute Virol, Russia .
    Gavrilova, Ludmila
    DI Ivanovskii Institute Virol, Russia .
    Kuzmina, Ksenia
    DI Ivanovskii Institute Virol, Russia .
    Mousavi-Jazi, Mehrdad
    Swedish Institute Communicable Disease Control, Sweden .
    Mirazimi, Ali
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
    Stroeher, Ute
    Centre Disease Control and Prevent, GA USA .
    Nichol, Stuart
    Centre Disease Control and Prevent, GA USA .
    Crimean-Congo Hemorrhagic Fever Virus, Greece2014In: Emerging Infectious Diseases, ISSN 1080-6040, E-ISSN 1080-6059, Vol. 20, no 2, p. 288-290Article in journal (Refereed)
    Abstract [en]

    Seroprevalence of Crimean-Congo hemorrhagic fever virus (CCHFV) is high in some regions of Greece, but only 1 case of disease has been reported. We used 4 methods to test 118 serum samples that were positive for CCHFV IgG by commercial ELISA and confirmed the positive results. A nonpathogenic or low-pathogenicity strain may be circulating.

  • 6.
    Rosenstierne, Maiken W.
    et al.
    Statens Serum Institut, Copenhagen, Denmark.
    McLoughlin, Kevin S.
    Lawrence Livermore National Laboratory, CA USA .
    Lindholm Olesen, Majken
    Statens Serum Institut, Copenhagen, Denmark.
    Papa, Anna
    Aristotle University of Thessaloniki, Greece .
    Gardner, Shea N.
    Lawrence Livermore National Laboratory, CA USA .
    Engler, Olivier
    Federal Office for Civil Protection, Spiez, Switzerland.
    Plumet, Sebastien
    French Army Forces Biomedical Institute (IRBA), Marseille, France.
    Mirazimi, Ali
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Swedish Institute for Communicable Disease Control, Solna, Sweden.
    Weidmann, Manfred
    University of Stirling, Scotland, UK.
    Niedrig, Matthias
    Robert Koch Institute, Berlin, Germany .
    Fomsgaard, Anders
    Statens Serum Institut, Copenhagen, Denmark; University of Southern Denmark, Odense.
    Erlandsson, Lena
    Statens Serum Institut, Copenhagen, Denmark.
    The Microbial Detection Array for Detection of Emerging Viruses in Clinical Samples - A Useful Panmicrobial Diagnostic Tool2014In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 6, p. e0100813-Article in journal (Refereed)
    Abstract [en]

    Emerging viruses are usually endemic to tropical and sub-tropical regions of the world, but increased global travel, climate change and changes in lifestyle are believed to contribute to the spread of these viruses into new regions. Many of these viruses cause similar disease symptoms as other emerging viruses or common infections, making these unexpected pathogens difficult to diagnose. Broad-spectrum pathogen detection microarrays containing probes for all sequenced viruses and bacteria can provide rapid identification of viruses, guiding decisions about treatment and appropriate case management. We report a modified Whole Transcriptome Amplification (WTA) method that increases unbiased amplification, particular of RNA viruses. Using this modified WTA method, we tested the specificity and sensitivity of the Lawrence Livermore Microbial Detection Array (LLMDA) against a wide range of emerging viruses present in both non-clinical and clinical samples using two different microarray data analysis methods.

1 - 6 of 6
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