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  • 1.
    Azharuddin, Mohammad
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för klinisk kemi. Linköpings universitet, Medicinska fakulteten.
    Zhu, Geyunjian H.
    Univ Cambridge, England.
    Das, Debapratim
    Indian Inst Technol Guwahati, India.
    Ozgur, Erdogan
    Hacettepe Univ, Turkey.
    Uzun, Lokman
    Hacettepe Univ, Turkey.
    Turner, Anthony P. F.
    Cranfield Univ, England.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Univ Cambridge, England.
    A repertoire of biomedical applications of noble metal nanoparticles2019Inngår i: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, Vol. 55, nr 49, s. 6964-6996Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Noble metals comprise any of several metallic chemical elements that are outstandingly resistant to corrosion and oxidation, even at elevated temperatures. This group is not strictly defined, but the tentative list includes ruthenium, rhodium, palladium, silver, osmium, iridium, platinum and gold, in order of atomic number. The emerging properties of noble metal nanoparticles are attracting huge interest from the translational scientific community and have led to an unprecedented expansion of research and exploration of applications in biotechnology and biomedicine. Noble metal nanomaterials can be synthesised both by top-down and bottom up approaches, as well as via organism-assisted routes, and subsequently modified appropriately for the field of use. Nanoscale analogues of gold, silver, platinum, and palladium in particular, have gained primary importance owing to their excellent intrinsic properties and diversity of applications; they offer unique functional attributes, which are quite unlike the bulk material. Modulation of noble metal nanoparticles in terms of size, shape and surface functionalisation has endowed them with unusual capabilities and manipulation at the chemical level, which can lead to changes in their electrical, chemical, optical, spectral and other intrinsic properties. Such flexibility in multi-functionalisation delivers Ockhams razor to applied biomedical science. In this feature article, we highlight recent advances in the adaptation of noble metal nanomaterials and their biomedical applications in therapeutics, diagnostics and sensing.

    Fulltekst tilgjengelig fra 2020-05-21 00:01
  • 2.
    Azzouzi, Sawsen
    et al.
    University of Sousse, Tunisia.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Ben Ali, Mounir
    University of Sousse, Tunisia.
    Nooredeen Abbas, Mohammed
    National Research Centre, Egypt.
    Dridi, Cherif
    Centre Research Microelect and Nanotechnol CRMN Sousse, Tunisia.
    Errachid, Abdelhamid
    University of Lyon 1, France.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Citrate-selective electrochemical mu-sensor for early stage detection of prostate cancer2016Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 228, s. 335-346Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The extremely specialised anatomical function of citrate inside the prostate, make it one of the preferred biomarkers for early stage detection of prostate cancer. However, current detection methods are seriously limited due to the very low citrate concentrations that need to be measured in order to follow disease progression. In the present work, we report a novel citrate-selective-sensor based on iron (III) phthalocyanine chloride-C-monoamido-Poly-n-Butyl Acrylate (Fe(III)MAPcC1 P n BA) modified gold -electrodes for the electrochemical determination and estimation of the pathophysiological range of citrate. The newly synthesised ionophore has been structurally characterised using Fourier transform infrared (FTIR) and UV-vis spectroscopy. Contact angle measurements and atomic force microscopy (AFM) have been used to investigate the adhesion and morphological properties of the membrane. The developed citrate-selective-electrodes had a Nernstian sensitivity of-19.34 +/- 0.83 mV/decade with a detection limit of about 9 x 10-6M and a linear range from 4 x 10(-5)M to 10(-1) M, which covered the pathologically important clinical range. Electrochemical impedance spectroscopy (EIS) showed very high sensitivity with a lower Limit of detection 1.7 x 10(-9) M and linear detection range (10(-8)-10(-1) M), which is very important not only for the early-stage diagnosis and screening procedures, but also in mapping the stage of the cancer too. (C) 2016 Elsevier B.V. All rights reserved.

  • 3.
    Bandyopadhyay, Souvik K.
    et al.
    GlaxoSmithKline Asia Pvt. Ltd., Bangalore, India.
    Azharuddin, Mohammad
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för klinisk kemi. Linköpings universitet, Medicinska fakulteten.
    Dasgupta, Anjan K.
    Department of Biochemistry, University of Calcutta, Kolkata, India.
    Ganguli, Bhaswati
    Department of Statistics, University of Calcutta, Kolkata, India.
    SenRoy, Sugata
    Department of Statistics, University of Calcutta, Kolkata, India.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Wolfson College, University of Cambridge, Cambridge, United Kingdom; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.
    Deb, Suryyani
    Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Kolkata, India.
    Probing ADP Induced Aggregation Kinetics During Platelet-Nanoparticle Interactions: Functional Dynamics Analysis to Rationalize Safety and Benefits2019Inngår i: Frontiers in Bioengineering and Biotechnology, E-ISSN 2296-4185, Vol. 7, s. 163-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Platelets, one of the most sensitive blood cells, can be activated by a range of external and internal stimuli including physical, chemical, physiological, and/or non-physiological agents. Platelets need to respond promptly during injury to maintain blood hemostasis. The time profile of platelet aggregation is very complex, especially in the presence of the agonist adenosine 5′-diphosphate (ADP), and it is difficult to probe such complexity using traditional linear dose response models. In the present study, we explored functional analysis techniques to characterize the pattern of platelet aggregation over time in response to nanoparticle induced perturbations. This has obviated the need to represent the pattern of aggregation by a single summary measure and allowed us to treat the entire aggregation profile over time, as the response. The modeling was performed in a flexible manner, without any imposition of shape restrictions on the curve, allowing smooth platelet aggregation over time. The use of a probabilistic framework not only allowed statistical prediction and inference of the aggregation signatures, but also provided a novel method for the estimation of higher order derivatives of the curve, thereby allowing plausible estimation of the extent and rate of platelet aggregation kinetics over time. In the present study, we focused on the estimated first derivative of the curve, obtained from the platelet optical aggregometric profile over time and used it to discern the underlying kinetics as well as to study the effects of ADP dosage and perturbation with gold nanoparticles. In addition, our method allowed the quantification of the extent of inter-individual signature variations. Our findings indicated several hidden features and showed a mixture of zero and first order kinetics interrupted by a metastable zero order ADP dose dependent process. In addition, we showed that the two first order kinetic constants were ADP dependent. However, we were able to perturb the overall kinetic pattern using gold nanoparticles, which resulted in autocatalytic aggregation with a higher aggregate mass and which facilitated the aggregation rate.

  • 4.
    Banerjee, Debarshi
    et al.
    Columbia University Medical Center, USA.
    Cieslar-Pobuda, Artur
    University of Oslo, Norway.
    Zhu, Geyunjian Harry
    University of Cambridge, UK.
    Wiechec, Emilia
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Patra, Hirak
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Department of Chemical Engineering and Biotechnology, University of Cambridge, UK; Wolfson College, University of Cambridge, UK.
    Adding nanotechnology to the metastasis treatment arsenal2019Inngår i: TIPS - Trends in Pharmacological Sciences, ISSN 0165-6147, E-ISSN 1873-3735, Vol. 40, nr 6, s. 403-418Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Metastasis is a major cause of cancer-related mortality, accounting for 90% of cancer deaths. The explosive growth of cancer biology research has revealed new mechanistic network information and pathways that promote metastasis. Consequently, a large number of antitumor agents have been developed and tested for their antimetastatic efficacy. Despite their exciting cytotoxic effects on tumor cells in vitro and antitumor activities in preclinical studies in vivo, only a few have shown potent antimetastatic activities in clinical trials. In this review, we provide a brief overview of current antimetastatic strategies that show clinical efficacy and review nanotechnology-based approaches that are currently being incorporated into these therapies to mitigate challenges associated with treating cancer metastasis.

    Fulltekst tilgjengelig fra 2020-05-07 12:06
  • 5.
    Chey, Chan Oeurn
    et al.
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska högskolan.
    Patra, Hirak K
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Tengdelius, Mattias
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska högskolan.
    Golabi, Mohsen
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Parlak, Onur
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Imani, Roghayeh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Elhag, Sami A. I.
    Linköpings universitet, Institutionen för teknik och naturvetenskap, Fysik och elektroteknik. Linköpings universitet, Tekniska högskolan.
    Yandi, Wetra
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Impact of nanotoxicology towards technologists to end users2013Inngår i: Advanced Materials Letters, ISSN 0976-3961, E-ISSN 0976-397X, Vol. 4, nr 8, s. 591-597Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The length scale for nanomaterial is small enough to be invisible and presume innocence for the initial avoidance of the toxicity issues. Again it was beyond the understanding of the time frame when nanotechnology just blooms that a length scale itself might be an important toxic parameter apart from its materialistic properties. We present this report to address the fundamental issues and questions related to the nanotoxicity issues from laboratory to the land of applications. We emphasize about the basic nanoscale materials that are regularly being used by the scientific community and the nanotechnology based materials that has already in the market or will come soon.

  • 6.
    Farahani, Ensieh
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Jangamreddy, Jaganmohan Reddy
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Rashedi, Iran
    University of Toronto, ON, Canada.
    Kawalec, Martha
    University of Manitoba, Winnipeg, Canada .
    Rao Pariti, Rama K.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Batakis, Petros
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska högskolan.
    Wiechec, Emilia
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Cell adhesion molecules and their relation to (cancer) cell stemness2014Inngår i: Carcinogenesis, ISSN 0143-3334, E-ISSN 1460-2180, Vol. 35, nr 4, s. 747-759Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Despite decades of search for anticancer drugs targeting solid tumors, this group of diseases remains largely incurable, especially if in advanced, metastatic stage. In this review, we draw comparison between reprogramming and carcinogenesis, as well as between stem cells (SCs) and cancer stem cells (CSCs), focusing on changing garniture of adhesion molecules. Furthermore, we elaborate on the role of adhesion molecules in the regulation of (cancer) SCs division (symmetric or asymmetric), and in evolving interactions between CSCs and extracellular matrix. Among other aspects, we analyze the role and changes of expression of key adhesion molecules as cancer progresses and metastases develop. Here, the role of cadherins, integrins, as well as selected transcription factors like Twist and Snail is highlighted, not only in the regulation of epithelial-to-mesenchymal transition but also in the avoidance of anoikis. Finally, we briefly discuss recent developments and new strategies targeting CSCs, which focus on adhesion molecules or targeting tumor vasculature.

  • 7.
    Lin, Qing
    et al.
    Sichuan Univ, Peoples R China; Univ Cambridge, England.
    Qu, Mengke
    Sichuan Univ, Peoples R China.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Univ Cambridge, England; Univ Cambridge, England.
    He, Shanshan
    Sichuan Univ, Peoples R China.
    Wang, Luyao
    Sichuan Univ, Peoples R China.
    Hua, Xun
    Sichuan Univ, Peoples R China; CQ MEDVT CO LTD, Peoples R China.
    Xiao, Linyu
    Sichuan Univ, Peoples R China.
    Fu, Yu
    Sichuan Univ, Peoples R China.
    Gong, Tao
    Sichuan Univ, Peoples R China.
    He, Qin
    Sichuan Univ, Peoples R China.
    Zhang, Ling
    Sichuan Univ, Peoples R China.
    Sun, Xun
    Sichuan Univ, Peoples R China.
    Zhang, Zhirong
    Sichuan Univ, Peoples R China.
    Mechanistic and therapeutic study of novel anti-tumor function of natural compound imperialine for treating non-small cell lung cancer2020Inngår i: Journal of Ethnopharmacology, ISSN 0378-8741, E-ISSN 1872-7573, Vol. 247, artikkel-id 112283Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Ethnopharmacological relevance: Bulbus Fritillaria cirrhosa D. Don (BFC) is a Chinese traditional herbal medicine that has long been used as an indispensable component in herbal prescriptions for bronchopulmonary diseases due to its well-established strong anti-inflammation and pulmonary harmonizing effects. Interestingly, there are few case reports in traditional Chinese medicine available where they found it to contribute in anti-tumor therapies. Imperialine is one of the most favored active substances extracted from BFC and has been widely recognized as an anti-inflammatory agent. Aim of the study: The aim of the current work is to provide first-hand evidences both in vitro and in vivo showing that imperialine exerts anti-cancer effects against non-small cell lung cancer (NSCLC), and to explore the molecular mechanism of this anti-tumor activity. It is also necessary to examine its systemic toxicity, and to investigate how to develop strategies for feasible clinical translation of imperialine. Materials and methods: To investigate anti-NSCLC efficacy of imperialine using both in vitro and in vivo methods where A549 cell line were chosen as in vitro model NSCLC cells and A549 tumor-bearing mouse model was constructed for in vivo study. The detailed underlying anti-cancer mechanism has been systematically explored for the first time through a comprehensive set of molecular biology methods mainly including immunohistochemistry, western blot and enzyme-linked immunosorbent assays. The toxicity profile of imperialine treatments were evaluated using healthy nude mice by examining hemogram and histopathology. An imperialine-loaded liposomal drug delivery system was developed using thin film hydration method to evaluate target specific delivery. Results: The results showed that imperialine could suppress both NSCLC tumor and associated inflammation through an inflammation-cancer feedback loop in which NF-kappa B activity was dramatically inhibited by imperialine. The NSCLC-targeting liposomal system was successfully developed for targeted drug delivery. The developed platform could favorably enhance imperialine cellular uptake and in vivo accumulation at tumor sites, thus improving overall anti-tumor effect. The toxicity assays revealed imperialine treatments did not significantly disturb blood cell counts in mice or exert any significant damage to the main organs. Conclusions: Imperialine exerts anti-cancer effects against NSCLC both in vitro and in vivo, and this previously unknown function is related to NF-kappa B centered inflammation-cancer feedback loop. Imperialine mediated anticancer activity is not through cytotoxicity and exhibit robust systemic safety. Furthermore, the liposome-based system we commenced would dramatically enhance therapeutic effects of imperialine while exhibiting extremely low side effects both on cellular and in NSCLC model. This work has identified imperialine as a promising novel anti-cancer compound and offered an efficient target-delivery solution that greatly facilitate practical use of imperialine.

  • 8.
    Lin, Qing
    et al.
    Sichuan Univ, Peoples R China; Univ Cambridge, England.
    Qu, Mengke
    Sichuan Univ, Peoples R China.
    Zhou, Bingjie
    Sichuan Univ, Peoples R China.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Univ Cambridge, England.
    Sun, Zihan
    Sichuan Univ, Peoples R China.
    Luo, Qiong
    Sichuan Univ, Peoples R China.
    Yang, Wenyu
    Sichuan Univ, Peoples R China.
    Wu, Yongcui
    Sichuan Univ, Peoples R China.
    Zhang, Yu
    Sichuan Univ, Peoples R China.
    Li, Lin
    Sichuan Univ, Peoples R China.
    Deng, Lang
    Sichuan Univ, Peoples R China.
    Wang, Leilei
    Sichuan Univ, Peoples R China.
    Gong, Tao
    Sichuan Univ, Peoples R China.
    He, Qin
    Sichuan Univ, Peoples R China.
    Zhang, Ling
    Sichuan Univ, Peoples R China.
    Sun, Xun
    Sichuan Univ, Peoples R China.
    Zhang, Zhirong
    Sichuan Univ, Peoples R China.
    Exosome-like nanoplatform modified with targeting ligand improves anti-cancer and anti-inflammation effects of imperialine2019Inngår i: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 311, s. 104-116Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Currently, most anti-cancer therapies are still haunted by serious and deleterious adverse effects. Here, we report a highly biocompatible tumor cell-targeting delivery systems utilizing exosome-like vesicles (ELVs) that delivers a low-toxicity anti-cancer agent imperialine against non-small cell lung cancer (NSCLC). First, we introduced a novel micelle-aided method to efficiently load imperialine into intact ELVs. Then, integrin alpha 3 beta 1-binding octapeptide cNGQGEQc was modified onto ELV platform for tumor targeting as integrin alpha 3 beta 1 is overexpressed on NSCLC cells. This system not only significantly improved imperialine tumor accumulation and retention, but also had extremely low systemic toxicity both in vitro and in vivo. Our discoveries offer new ways to utilize ELV more efficiently for both drug loading and targeting. The solid pharmacokinetics improvement and extraordinary safety of this system also highlight possibilities of alternative long course cancer therapies using similar strategies.

  • 9.
    Ozgur, Edogan
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Turkey.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Uzun, Lokman
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Hacettepe University, Turkey.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Smart polymerisable terbium (III) complex-based fluorescent MIP nanoparticles2016Inngår i: Biosensors 2016 – The World Congress on Biosensors, Gothenburg, Sweden, 25-27 May 2016, Elsevier, 2016Konferansepaper (Annet vitenskapelig)
  • 10.
    Patra, Hirak
    et al.
    University of Calcutta, Kolkata, India.
    Dasgupta, Anjan
    University of Calcutta, Kolkata, India.
    Cancer cell response to nanoparticles: criticality and optimality2012Inngår i: Nanomedicine: Nanotechnology, Biology and Medicine, ISSN 1549-9634, E-ISSN 1549-9642, Vol. 8, nr 6, s. 841-852Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A stochastic variation in size and electrical parameters is common in nanoparticles. Synthesizing gold nanoparticles with a varying range of size and zeta potential, we show that there is clustering at certain regions of hydrodynamic diameter and zeta potentials that can be classified using k-clustering technique. A cluster boundary was observed around 50 nm, a size known for its optimal response to cells. However, neither size nor zeta potential alone determined the optimal cellular response (e.g., percentage cell survival) induced by such nanoparticles. A complex interplay prevails between size, zeta potential, nature of surface functionalization, and extent of adhesion of the cell to a solid matrix. However, it follows that the ratio of zeta potential to surface area, which scales as the electrical field (by Gaussian law), serves as an appropriate indicator for optimal cellular response. The phase plot spanned by fractional survival and effective electric field (charge density) indicates a positive correlation between mean cell survival and the magnitude of the electric field. The phase plot spanned by fractional survival and effective electric field (charge density) associated with the nanosurface shows a bifurcation behavior. Wide variation of cell survival response is observed at certain critical values of the surface charge density, whereas in other ranges the cellular response is well behaved and more predictable. Existence of phase points near the critical region corresponds to wide fluctuation of nanoparticle-induced response, for small changes in the nanosurface property. Smaller nanoparticles with low zeta potential (e.g., those conjugated with arginine) can have such an attribute (i.e., higher electrical field strength), and eventually they cause more cell death. The study may help in optimal design of nanodrugs.

  • 11.
    Patra, Hirak
    et al.
    Department of Biochemistry, University of Calcutta, India.
    Dasgupta, Anjan Kr.
    Department of Biochemistry, University of Calcutta, India.
    Gold Nanostructures and their Applications in Diagnosis and Therapy of Cancer2012Inngår i: Nanotechnology: diagnosis and treatment of cancers / [ed] Rinti Banerjee, New Delhi, India: Narosa Publishing House, 2012, s. 27-35Kapittel i bok, del av antologi (Annet vitenskapelig)
    Abstract [en]

    The molecular level scenario of cancer is recently rescaled from interactive molecular interaction maps to network representation and dynamic modeling (Stromback et al.**). The rescaling is primarily motivated by a systems biology approach (Gitenkunst et al 2007; Pribyl**). The future of nanoparticle based biological research perhaps lies in such systems based picture of interaction of nanoparticles with cells and their different compartments.

    At present there are two distinct directions in which one finds prospects of nanoparticle based research in biology. Development of novel nano-probes used either for imaging or for therapeutic purpose and each of these perspectives are intricately related to the signaling networks existing in cancerous or normal cells (Irish et al., 2006). The nanotechnology approach can enable controlled perturbation in cell and intercellular compartments. In addition this will enable, efficient extraction of image based information of the cellular and sub cellular function, and design of drugs particularly in cases in which the targeted delivery is intended (Hu et al., 2007).

    Thus, nanotechnology based approaches are fast emerging as efficient aids to in vitro diagnostic techniques and to therapeutic procedures. The focus of this chapter is to concentrate on approaches in nanotechnology based diagnosis. This would be followed up by some discussions on formulation of nano-therapeutic strategy where specialized issues like toxicity and their significance would be touched-upon (Yadav et al, 2009).  The hypotheses with which the nanotechnology is applied in cancer research are (a) Early stage detection is very difficult due to less phenotypic expressions. (b) Most of the anticancer drugs cannot differentiate greatly between normal and cancerous cells. While the first point is pivotal in the diagnostic approaches, the second aspect is more relevant with respect to nano-based therapeutic procedures.

    Advanced technologies for tumor imaging, early detection, new methods for accurate diagnosis and prognosis are components of the former. The second issue, namely the targeted therapy has been subject of keen interest to a large section of researchers. Introduction of processes like laser or superficial X-ray irradiation in which nanoparticles may cause targeted and specific killing is an encouraging aspect of its use. Safety issues of nanoparticle-based drugs, their biodegradation or exclusion are the issues that have to develop side by side so as to make nano drugs acceptable in medicine. Other than this, special progresses in the treating aggressive and lethal cancers such as bone metastasis and development of nanomaterials that can be accessible to brain and other inaccessible tissues are some of the additional aspects that need a detailed consideration. A third equally important perspective exists for nanoparticle based sensing. This aspect is especially important for plasmonic nanoparticles like gold colloids.

  • 12.
    Patra, Hirak
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Imani, Roghayeh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Iglic, Ales
    Biophysics Laboratory, Faculty of Electrical Engineering, University of Ljubljana, Slovenia.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Novel anti-neoplastic approach for photodynamic theranostics by biocompatible TiO2 popcorn nanostructure for a high-throughput flash ROS generator2014Inngår i: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Reactive oxygen species (ROS) are important secondary messengers in the intracellular signaling system for regulating redox homeostasis in normal cells. Compared to normal cells, cancer cells have increased ROS levels due to a faster metabolic rate. We have used this discriminating overproduction of ROS levels in cancer cells  as a target for a photodynamic anti-neoplastic theranostic approach using mesoporous TiO2 microbeads with a popcorn nanostructure. We have created a novel flash ROS generator  using a two-step procedure consisting of sol-gel and solvothermal processes to obtain mesoporous TiO2 microbeads with high photocatalytic efficiency. A photon-induced comparative study has been carried out for the ROS generation ability using TiO2 nanoparticles and mesoporous TiO2 microbeads.  We have shown that in under otherwise identical conditions the extent of photocatalytical ROS generated by mesoporous TiO2 microbeads is more than twice that produced by TiO2 nanoparticles. In vitro in the absence of irradiation, the mesoporous TiO2 microbeads are exceptionally biocompatible, allowing almost ~100% cellular survival rate even at a dose of 100 µg/mL. In contrast, commercial nanoparticles showed concentration dependent cytotoxicity of nearly 15% within 24h in the absence of any irradiation. Upon photo activation, the mesoporous TiO2 microbead structures delivered their potential anticancer effect by interfering with the mitochondrial activity by producing ROS in the intracellular environment and thus reducing the survival rate of cells by more than 30% in comparison with commercial nanoparticles, where only an increase of 5% in cell death was observed. Thus we have developed a smart on/off switchable photodynamic anti-neoplastic theranostic approach that can be combined with specific cell recognition elements for future cancer management.

  • 13.
    Patra, Hirak K.
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    The potential legacy of cancer nanotechnology: celluar selection2014Inngår i: Trends in Biotechnology, ISSN 0167-7799, E-ISSN 1879-3096, Vol. 32, nr 1, s. 21-31Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Overexpression of oncogenes or loss of tumour suppressors can transform a normal cell to a cancerous one, resulting in uncontrolled regulation of intracellular signalling pathways and immunity to stresses, which both pose therapeutic challenges. Conventional approaches to cancer therapy, although they are effective at killing cancer cells, may still fail due to inadequate biodistribution and unwanted side effects. Nanotechnology-based approaches provide a promising alternative, with the possibility of targeting cells at an early stage, during their transformation into cancer cells. This review considers techniques that specifically target those molecular changes, which begin in only a very small percentage of normal cells as they undergo transformation. These techniques are crucial for early-stage diagnosis and therapy.

  • 14.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Univ Cambridge, England; Univ Cambridge, England.
    Sensible label-free bio-sensing: Towards in situ biopsy2018Inngår i: 2018 IEEE SENSORS, IEEE , 2018, s. 1721-1724Konferansepaper (Fagfellevurdert)
    Abstract [en]

    Integrative in situ bio-sensing approaches became ominous to advance early stage detection of complex and heterogeneous disease like cancer, chronic lung, inflammation, coronary syndromes etc. Particularly in cancer, we can save million lives with existing therapeutic strategies if we can detect them at their very early stage. Unfortunately, cancer offers very rare distinctly identifiable cues during its onset to identify them and posing the technological challenges to spot them at their very early stage. We have developed an aptamer based integrated switchable rare event sensing platform using variable angle spectroscopic ellipsometry to spot in situ, readout and monitor rare events in real-time in complex heterogeneous cell population. The developed aptasensors is being tested with spotting prostate cancer cell PC3 in a blood mimicking population of human peripheral blood mononuclear cells (PBMC). The proof-of-concept data showed that the developed platform can detect in situ up to 10 target cells/ml with 10 background cells. The integrated sensor is designed with switchable surface to safely capture and release the target cells for further clinical validation and analysis for advanced diagnosis. We envision this alternate integrated bio-sensors strategy will be an important step towards the development of label-free in situ biopsy technique for rare event detection and early stage detection of cancer.

  • 15.
    Patra, Hirak Kumar
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge, UK; Wolfson College, University of Cambridge, Cambridge, UK.
    Azharuddin, Mohammad
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för klinisk kemi. Linköpings universitet, Medicinska fakulteten.
    Islam, Mohammad Mirazul
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, USA.
    Papapavlou, Georgia
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för neuro- och inflammationsvetenskap. Linköpings universitet, Medicinska fakulteten.
    Deb, Suryyani
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Medicinska fakulteten. Department of Biochemistry, University of Calcutta, Calcutta, India; Department of Biotechnology, Maulana Abul Kalam Azad University of Technology (MAKAUT), West Bengal, India.
    Osterrieth, Johannes
    Department of Chemical Engineering and Biotechnology, Cambridge University, Philippa Fawcett Drive, Cambridge, UK.
    Zhu, Geyunjian Harry
    Department of Chemical Engineering and Biotechnology, Cambridge University, Philippa Fawcett Drive, Cambridge, UK.
    Romu, Thobias
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV.
    Dhara, Ashis K.
    Centre for Image Analysis, Uppsala University, Uppsala, Sweden; Department of Electrical Engineering, National Institute of Technology Durgapur, West Bengal, India.
    Jafari, Mohammad Javad
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Gadheri, Amineh
    Department of Oncology‐Pathology, Karolinska Institute, Stockholm, Sweden.
    Hinkula, Jorma
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för hematopoes och utvecklingsbiologi. Linköpings universitet, Medicinska fakulteten.
    Rajan, Madhavan S.
    Department of Ophthalmology, Cambridge University Hospitals NHS Trust and Vision and Eye Research Institute (VERI), Anglia Ruskin University, Cambridge, UK.
    Slater, Nigel K. H.
    Department of Chemical Engineering and Biotechnology, Cambridge University, Philippa Fawcett Drive, Cambridge, UK.
    Rational Nanotoolbox with Theranostic Potential for Medicated Pro-Regenerative Corneal Implants2019Inngår i: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, artikkel-id 1903760Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Cornea diseases are a leading cause of blindness and the disease burden is exacerbated by the increasing shortage around the world for cadaveric donor corneas. Despite the advances in the field of regenerative medicine, successful transplantation of laboratory‐made artificial corneas is not fully realized in clinical practice. The causes of failure of such artificial corneal implants are multifactorial and include latent infections from viruses and other microbes, enzyme overexpression, implant degradation, extrusion or delayed epithelial regeneration. Therefore, there is an urgent unmet need for developing customized corneal implants to suit the host environment and counter the effects of inflammation or infection, which are able to track early signs of implant failure in situ. This work reports a nanotoolbox comprising tools for protection from infection, promotion of regeneration, and noninvasive monitoring of the in situ corneal environment. These nanosystems can be incorporated within pro‐regenerative biosynthetic implants, transforming them into theranostic devices, which are able to respond to biological changes following implantation.

    Fulltekst tilgjengelig fra 2020-07-15 00:01
  • 16.
    Patra, Hirak Kumar
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Imani, Roghayeh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. Univ,of Ljubljana, Slovenia; University of Ljubljana, Slovenia.
    Jangamreddy, Jaganmohan
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Pazoki, Meysam
    Uppsala University, Sweden.
    Iglic, Ales
    University of Ljubljana, Slovenia.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten. Tekidag AB, SE-58330 Linkoping, Sweden.
    On/off-switchable anti-neoplastic nanoarchitecture2015Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, nr 14571, s. 1-9Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Throughout the world, there are increasing demands for alternate approaches to advanced cancer therapeutics. Numerous potentially chemotherapeutic compounds are developed every year for clinical trial and some of them are considered as potential drug candidates. Nanotechnology-based approaches have accelerated the discovery process, but the key challenge still remains to develop therapeutically viable and physiologically safe materials suitable for cancer therapy. Here, we report a high turnover, on/off-switchable functionally popping reactive oxygen species (ROS) generator using a smart mesoporous titanium dioxide popcorn (TiO2 Pops) nanoarchitecture. The resulting TiO2 Pops, unlike TiO2 nanoparticles (TiO2 NPs), are exceptionally biocompatible with normal cells. Under identical conditions, TiO2 Pops show very high photocatalytic activity compared to TiO2 NPs. Upon on/off-switchable photo activation, the TiO2 Pops can trigger the generation of high-turnover flash ROS and can deliver their potential anticancer effect by enhancing the intracellular ROS level until it crosses the threshold to open the death gate, thus reducing the survival of cancer cells by at least six times in comparison with TiO2 NPs without affecting the normal cells.

  • 17.
    Patra, Hirak Kumar
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Khaliq, Nisar Ul
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Romu, Thobias
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Wiechec, Emilia
    Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi.
    Borga, Magnus
    Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV. Linköpings universitet, Institutionen för medicinsk teknik, Medicinsk informatik. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony P. F.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    MRI-Visual Order–Disorder Micellar Nanostructures for Smart Cancer Theranostics2014Inngår i: Advanced Healthcare Materials, ISSN 2192-2640, Vol. 3, nr 4, s. 526-535Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The development of MRI-visual order–disorder structures for cancer nanomedicine explores a pH-triggered mechanism for theragnosis of tumor hallmark functions. Superparamagnetic iron oxide nanoparticles (SPIONs) stabilized with amphiphilic poly(styrene)-b-poly(acrylic acid)-doxorubicin with folic acid (FA) surfacing are employed as a multi-functional approach to specifically target, diagnose, and deliver drugs via a single nanoscopic platform for cancer therapy. The functional aspects of the micellar nanocomposite is investigated in vitro using human breast SkBr3 and colon cancer HCT116 cell lines for the delivery, release, localization, and anticancer activity of the drug. For the first time, concentration-dependent T2-weighted MRI contrast for a monolayer of clustered cancer cells is shown. The pH tunable order–disorder transition of the core–shell structure induces the relative changes in MRI contrast. The outcomes elucidate the potential of this material for smart cancer theranostics by delivering non-invasive real-time diagnosis, targeted therapy, and monitoring the course and response of the action before, during, and after the treatment regimen.

  • 18.
    Patra, Hirak Kumar
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik.
    Sharma, Yashpal
    International Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Sengen, Tsukuba, Ibaraki, Japan .
    Islam, Mohammad Mirazul
    Swedish Nanoscience Center, Karolinska Institute, Stockholm, Sweden.
    Jafari, Mohammad Javad
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Arul Murugan, N.
    Virtual Laboratory for Molecular Probes, Division of Theoretical Chemistry and Biology, School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden .
    Kobayashi, Hisatoshi
    International Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Sengen, Tsukuba, Ibaraki, Japan .
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten. International Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Sengen, Tsukuba, Ibaraki, Japan ; Tekidag AB, UCS, Linköping, Sweden; Vinoba Bhave Research Institute, Saidabad, Allahabad, India .
    Inflammation-sensitive in situ smart scaffolding for regenerative medicine2016Inngår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, nr 39, s. 17213-17222Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    To cope with the rapid evolution of the tissue engineering field, it is now essential to incorporate the use of on-site responsive scaffolds. Therefore, it is of utmost importance to find new Intelligent biomaterials that can respond to the physicochemical changes in the microenvironment. In this present report, we have developed biocompatible stimuli responsive polyaniline-multiwalled carbon nanotube/poly(N-isopropylacrylamide), (PANI-MWCNT/PNIPAm) composite nanofiber networks and demonstrated the physiological temperature coordinated cell grafting phenomenon on its surface. The composite nanofibers were prepared by a two-step process initiated with an assisted in situ polymerization followed by electro-spinning. To obtain a smooth surface in individual nanofibers with the thinnest diameter, the component ratios and electrospinning conditions were optimized. The temperature-gated rearrangements of the molecular structure are characterized by FTIR spectroscopy with simultaneous macromolecular architecture changes reflected on the surface morphology, average diameter and pore size as determined by scanning electron microscopy. The stimuli responsiveness of the nanofibers has first been optimized with computational modeling of temperature sensitive components (coil-like and globular conformations) to tune the mechanism for temperature dependent interaction during in situ scaffolding with the cell membrane. The nanofiber networks show excellent biocompatibility, tested with fibroblasts and also show excellent sensitivity to inflammation to combat loco-regional acidosis that delay the wound healing process by an in vitro model that has been developed for testing the proposed responsiveness of the composite nanofiber networks. Cellular adhesion and detachment are regulated through physiological temperature and show normal proliferation of the grafted cells on the composite nanofibers. Thus, we report for the first time, the development of physiological temperature gated inflammation-sensitive smart biomaterials for advanced tissue regeneration and regenerative medicine.

  • 19.
    Patra, Hirak Kumar
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Valyukh, Sergiy
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Tillämpad optik. Linköpings universitet, Tekniska fakulteten.
    Wiechec, Emilia
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för neuro- och inflammationsvetenskap. Linköpings universitet, Medicinska fakulteten.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Switchable label free apta-nanosensor: In situ biopsy?2016Inngår i: Biosensors 2016 – The World Congress on Biosensors, Gothenburg, Sweden, 25-27 May 2016, Elsevier, 2016Konferansepaper (Annet vitenskapelig)
  • 20.
    Patra, Hirak
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Tiwari, Ashutosh
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Smart inflammation sensitive self-reporting theragnosis2014Inngår i: FEBS-EMBO 2014 Congress, 2014Konferansepaper (Annet vitenskapelig)
    Abstract [en]

    We have designed and develop a novel class of nanocomposites for inflammation based hallmark functions using biocompatible metallic nano-objects (SPION, nanorod) assembled with a pH sensitive amphiphilic azide terminated block polymer, polystyrene-b-poly (acrylic acid) and temperature-responsive polymer Poly (N-isopropylacrylamide) (PNIPAAm) in a single nanoscopic platform. The nano-architecture is a uniform core-shell micellar assembly of polymer around the biocompatible metallic core. Doxorubicin and methotrexate are loaded within the architecture as the model therapeutic module. The drugs are linked through pH and enzyme sensitive bonds. The complete nano-architecture and linkages are characterized by electron microscopy, NMR and Photon Correlation Spectroscopy. The drug release response has been optimized with different cell line in vitro. The model suggest that change/increase in temperature, reduction of pH and the redox enzymatic activities are increased at the localized inflammatory sites, can be addressed by the developed module and the drug will be released at the inflammation sites only due to their specific linkage to the module. Again we have explored order–disorder micellar structures dependent T1 & T2 MRI properties of the module. This results indicate that the fabricated module can also be useful not only probing the inflammation site non invasively through MRI but also will give us idea on the extent of release of drugs at the inflammation sites. The outcomes of these results elucidate the potential of this fabricated nano-architecture for smart theranostics through physicochemical and microenvironment feature based drug delivery, site-specific therapy, real-time probing and non-invasive monitoring of the drug action course for personalized therapy.

     

  • 21.
    Ravichandran, Ranjithkumar
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Åstrand, C.
    KTH Royal Institute Technology, Sweden.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Chotteau, V.
    KTH Royal Institute Technology, Sweden.
    Phopase, Jaywant
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Intelligent ECM mimetic injectable scaffolds based on functional collagen building blocks for tissue engineering and biomedical applications2017Inngår i: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 7, nr 34, s. 21068-21078Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Hydrogels comprising natural extracellular matrix (ECM) components are very attractive as scaffolds for regenerative medicine applications due to their inherent biointeractive properties. Responsive materials that adapt to their surrounding environments and regulate transport of ions and bioactive molecules manifest significant advantages for biomedical applications. Although there are many exciting challenges, the opportunity to design, fabricate and engineer stimuli-responsive polymeric systems based on ECM components is particularly attractive for regenerative medicine. Here we describe a one-pot approach to fabricate in situ fast gellable intelligent ECM mimetic scaffolds, based on methacrylated collagen building blocks with mechanical properties that can be modulated in the kPa-MPa range and that are suitable for both soft and hard tissues. Physiochemical characterizations demonstrate their temperature and pH responsiveness, together with the structural and enzymatic resistance that make them suitable scaffolds for long-term use in regenerative medicine and biomedical applications. The multifunctionality of these hydrogels has been demonstrated as an in situ depot-forming delivery platform for the adjustable controlled release of proteins and small drug molecules under physiological conditions and as a structural support for adhesion, proliferation and metabolic activities of human cells. The results presented herein should be useful to the design and fabrication of tailor-made scaffolds with tunable properties that retain and exhibit sustained release of growth factors for promoting tissue regeneration.

  • 22.
    Tiwari, Ashutosh
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Patra, HirakLinköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.Turner, AnthonyLinköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Advanced Bioelectronic Materials2015Collection/Antologi (Annet vitenskapelig)
    Abstract [en]

    This book covers the recent advances in the development of bioelectronics systems and their potential application in future biomedical applications starting from system design to signal processing for physiological monitoring, to in situ biosensing.

    Advanced Bioelectronics Materialshas contributions from distinguished international scholars whose backgrounds mirror the multidisciplinary readership ranging from the biomedical sciences, biosensors and engineering communities with diverse backgrounds, interests and proficiency in academia and industry. The readers will benefit from the widespread coverage of the current literature, state-of-the-art overview of all facets of advanced bioelectronics materials ranging from real time monitoring, in situ diagnostics, in vivo imaging, image-guided therapeutics, biosensors, and translational biomedical devices and personalized monitoring.

  • 23.
    Tiwari, Ashutosh
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Patra, Hirak
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Preface2015Inngår i: Advanced bioelectronics materials / [ed] Ashutosh Tiwari, Hirak Patra and Anthony Turner, Beverly, MA, USA: Wiley-Scrivener , 2015, s. XV-Kapittel i bok, del av antologi (Annet vitenskapelig)
  • 24.
    Yilmaz, Erkut
    et al.
    Aksaray University, Turkey.
    Garipcan, Bora
    Bogazici University, Turkey.
    Patra, Hirak Kumar
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Uzun, Lokman
    Hacettepe University, Turkey.
    Molecular Imprinting Applications in Forensic Science2017Inngår i: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 17, nr 4, artikkel-id 691Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Producing molecular imprinting-based materials has received increasing attention due to recognition selectivity, stability, cast effectiveness, and ease of production in various forms for a wide range of applications. The molecular imprinting technique has a variety of applications in the areas of the food industry, environmental monitoring, and medicine for diverse purposes like sample pretreatment, sensing, and separation/purification. A versatile usage, stability and recognition capabilities also make them perfect candidates for use in forensic sciences. Forensic science is a demanding area and there is a growing interest in molecularly imprinted polymers (MIPs) in this field. In this review, recent molecular imprinting applications in the related areas of forensic sciences are discussed while considering the literature of last two decades. Not only direct forensic applications but also studies of possible forensic value were taken into account like illicit drugs, banned sport drugs, effective toxins and chemical warfare agents in a review of over 100 articles. The literature was classified according to targets, material shapes, production strategies, detection method, and instrumentation. We aimed to summarize the current applications of MIPs in forensic science and put forth a projection of their potential uses as promising alternatives for benchmark competitors.

1 - 24 of 24
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