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  • 1.
    Ali Malik, Muhammad
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    Gatto, Emanuela
    University Roma Tor Vergata.
    Macken, Stephen
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    DiNatale, Corrado
    University Roma Tor Vergata.
    Paolesse, Roberto
    University Roma Tor Vergata.
    DAmico, Arnaldo
    University Roma Tor Vergata.
    Lundström, Ingemar
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Imaging fingerprinting of excitation emission matrices2009In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 635, no 2, p. 196-201Article in journal (Refereed)
    Abstract [en]

    The spectral fingerprinting of the excitation emission matrix (EEM) of fluorescent substances is demonstrated using polychromatic light sources and tri-chromatic image detectors. A model of the measured fingerprints explaining their features and classification performance, based on the polychromatic excitation of the indicators is proposed.

    Substantial amount of spectral information is retained in the fingerprints as corroborated by multivariate analysis and experimental conditions that favor such situation are identified.

    In average, for five different substances, the model shows a fitting goodness measured by the Pearsons r coefficient and the root mean square deviation of 0.8541 and 0.0247 respectively, while principal component classification patterns satisfactorily compare with the EEM spectroscopy classification and respectively explain 96% and 93% of the information in the fist two principal components.

    The measurements can be performed using regular computer screens as illumination and web cameras as detectors, which constitute ubiquitous and affordable platforms compatible with distributed evaluations, in contrast to regular instrumentation for EEM measurements.

  • 2.
    Fallqvist, Amie
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Macken, Stephen
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Optically addressable field effect nano-environments for chemical imaging2009In: Procedia Chemistry, ISSN 1876-6196, Vol. 1, no 1, p. 224-227Article in journal (Refereed)
    Abstract [en]

    An optically addressable field effect device Incorporating functionalized nano-environments is demonstrated for the generation of multiple response signatures of a same analyte-sensing material combination. The device can operate unsupervised and different response mechanism can be simultaneously tested and incorporated in a multidimensional response. This proof of concept explores the well-known Pd-H-2 case, while the concept is a breadboard for chemical imaging experiments.

  • 3.
    Lundström, Ingemar
    et al.
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Filippini, Daniel
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Macken, Stephen
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
    Optical properties of microstructures for computer screen photo-assisted experiments2006In: Eurosensors XX,2006, 2006Conference paper (Other academic)
    Abstract [en]

          

  • 4.
    Lundström, Ingemar
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
    Macken, Stephen
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
    Filippini, Daniel
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Optical properties of microstructures for computer screen photoassisted experiments2006In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 89, no 25Article in journal (Refereed)
    Abstract [en]

    SU-8 microstructured devices aiding the fluorescence detection in computer screen photoassisted experiments are demonstrated. The structures exploit computer screens as controlled light sources and web cameras as imaging detectors, separating the excitation by total internal reflection and redirecting surface generated fluorescence toward the detector. The devices are essentially disposable and allow the collection of up to five times larger fluorescent signals, while remaining compatible with the formation of complex response patterns and with fluidic functions.

  • 5.
    Macken, Stephen
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Integrated Optical Solutions for Ubiquitous Sensing2010Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Ubiquitous chemical sensing aims at exploiting consumer electronic devices as temporary chemical sensing platforms. These devices are a highly disseminated infrastructure, not presently being exploited for chemical sensing purposes. The goal of the ubiquitous chemical sensing field is to create the methodologies and adapted devices to profit from this unexploited resource.

    DVD drives, flat bed scanners and computer screens used in combination with web cameras, and mobile phones have been demonstrated as measuring platforms, during the past 10 years.

    In particular the combination of computer screens with web cameras, a technique called the computer screen photo-assisted technique (CSPT), is the most versatile approach to support multiple types of optical detection phenomena, and together with mobile phones are the most ubiquitous type of platforms.

    The versatility of CSPT comes from the ease with which one can configure a spectrally controlled, 2D micro-positioned, linearly polarized, wide angle and internally modulated illumination with a programmable screen, just by controlling the displayed contents. Concurrently, the imaging devices in CSPT enable one to capture arbitrary assay layouts, the evaluation of which can then be numerically customized.

    The full exploitation of the CSPT platform requires optically adapted sensing interfaces and specialized methods, in order to increase sensitivity and make use of all available information, for a thorough and complete chemical sensing analysis. In this thesis I have explored the foundations of CSPT sensing on integrated devices, developing methods and sensing’s interfaces that provide enhanced optical detection customized to the CSPT platform.

    List of papers
    1. Optical properties of microstructures for computer screen photoassisted experiments
    Open this publication in new window or tab >>Optical properties of microstructures for computer screen photoassisted experiments
    2006 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 89, no 25Article in journal (Refereed) Published
    Abstract [en]

    SU-8 microstructured devices aiding the fluorescence detection in computer screen photoassisted experiments are demonstrated. The structures exploit computer screens as controlled light sources and web cameras as imaging detectors, separating the excitation by total internal reflection and redirecting surface generated fluorescence toward the detector. The devices are essentially disposable and allow the collection of up to five times larger fluorescent signals, while remaining compatible with the formation of complex response patterns and with fluidic functions.

    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:liu:diva-37633 (URN)10.1063/1.2423246 (DOI)36905 (Local ID)36905 (Archive number)36905 (OAI)
    Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
    2. Towards integrated devices for computer screen photo-assisted multi-parameter sensing
    Open this publication in new window or tab >>Towards integrated devices for computer screen photo-assisted multi-parameter sensing
    Show others...
    2009 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 632, no 1, p. 143-147Article in journal (Refereed) Published
    Abstract [en]

    The computer screen photo-assisted technique (CSPT) utilizes consumer electronic devices such as computer screens and web cameras for distributed chemical sensing. Key to this method is the development of small and disposable sensing assays able to aid the CSPT optical detection, and the evaluation of multiple indicators. Here we demonstrate CSPT identification of fluorescent indicators from individual 100 mu m SU-8 (10) pillars covering an area of 4 mm x 4 mm with a density of 47 elements mm-2. The extraction of distinctive spectral fingerprints is aided by the exploitation of a three-band Moire interference that enables the partial Bayer decoding of the CSPT images.

    Keywords
    Optical sensor, SU-8, Microstructures, Moire
    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:liu:diva-16633 (URN)10.1016/j.aca.2008.10.076 (DOI)
    Available from: 2009-02-08 Created: 2009-02-06 Last updated: 2017-12-14Bibliographically approved
    3. Imaging fingerprinting of excitation emission matrices
    Open this publication in new window or tab >>Imaging fingerprinting of excitation emission matrices
    Show others...
    2009 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 635, no 2, p. 196-201Article in journal (Refereed) Published
    Abstract [en]

    The spectral fingerprinting of the excitation emission matrix (EEM) of fluorescent substances is demonstrated using polychromatic light sources and tri-chromatic image detectors. A model of the measured fingerprints explaining their features and classification performance, based on the polychromatic excitation of the indicators is proposed.

    Substantial amount of spectral information is retained in the fingerprints as corroborated by multivariate analysis and experimental conditions that favor such situation are identified.

    In average, for five different substances, the model shows a fitting goodness measured by the Pearsons r coefficient and the root mean square deviation of 0.8541 and 0.0247 respectively, while principal component classification patterns satisfactorily compare with the EEM spectroscopy classification and respectively explain 96% and 93% of the information in the fist two principal components.

    The measurements can be performed using regular computer screens as illumination and web cameras as detectors, which constitute ubiquitous and affordable platforms compatible with distributed evaluations, in contrast to regular instrumentation for EEM measurements.

    Keywords
    Chemical sensing, Optical sensing, Excitation emission matrix, Computer screen photo-assisted technique, Spectral fingerprinting
    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:liu:diva-17292 (URN)10.1016/j.aca.2009.01.018 (DOI)
    Available from: 2009-03-16 Created: 2009-03-16 Last updated: 2017-12-13
    4. Monolithic SU-8 Microcavities for Efficient Fluorescence Collection
    Open this publication in new window or tab >>Monolithic SU-8 Microcavities for Efficient Fluorescence Collection
    2009 (English)In: Procedia Chemistry, ISSN 1876-6196, Vol. 1, no 1, p. 1115-1118Article in journal (Refereed) Published
    Abstract [en]

    The fabrication of capped microstructures such as channels or cavities typically involves multiple production steps. In this work we demonstrate a fabrication procedure that enables the generation of capped monolithic microstructures of arbitrary geometry in one single exposure step. The presented method also enables the embedment of metal self-aligned surfaces for use as electrodes or mirrors. The devices furthermore demonstrate a capability of increasing fluorescent collection, as measured by an epifluorescent inverted microscope, by up to 15 fold.

    Place, publisher, year, edition, pages
    ELSEVIER SCIENCE BV, 2009
    Keywords
    Fluorescence; microfluidics; SU-8; Fresnel diffraction
    National Category
    Engineering and Technology
    Identifiers
    urn:nbn:se:liu:diva-58422 (URN)10.1016/j.proche.2009.07.278 (DOI)000275995600056 ()
    Note

    Proceedings of the Eurosensors XXIII conference.

    Available from: 2010-08-12 Created: 2010-08-11 Last updated: 2015-06-01
    5. Colour moire interferometry for physical sensing using a standard web camera
    Open this publication in new window or tab >>Colour moire interferometry for physical sensing using a standard web camera
    2010 (English)In: Measurement science and technology, ISSN 0957-0233, E-ISSN 1361-6501, Vol. 21, no 10, p. 105305-Article in journal (Refereed) Published
    Abstract [en]

    Digital moire patterns are an effect seen in digital pictures when a high-contrast periodic object being imaged interferes with the periodic image detector in the camera. This is often an undesired effect, which is usually removed through various filtering processes. However in this work we demonstrate the ability to use a deliberately created moire pattern to measure the physical distance of an object from a web camera with a resolution of 131 mu m. This measurement principle is then adapted to measure weight as a ubiquitous scale with a resolution of 240 mu g.

    Place, publisher, year, edition, pages
    Iop Publishing Ltd, 2010
    Keywords
    interference, speckle and moire patterns, image forming and processing, optoelectronic device characterization, design and modelling
    National Category
    Engineering and Technology
    Identifiers
    urn:nbn:se:liu:diva-60233 (URN)10.1088/0957-0233/21/10/105305 (DOI)000281787100022 ()
    Note

    Original Publication: Stephen Macken and Daniel Filippini, Colour moiré interferometry for physical sensing using a standard web camera, 2010, MEASUREMENT SCIENCE and TECHNOLOGY, (21), 10, 105305. http://dx.doi.org/10.1088/0957-0233/21/10/105305 Copyright: Iop Publishing Ltd http://www.iop.org/

    Available from: 2010-10-08 Created: 2010-10-08 Last updated: 2017-12-12
    6. Optically addressable field effect nano-environments for chemical imaging
    Open this publication in new window or tab >>Optically addressable field effect nano-environments for chemical imaging
    2009 (English)In: Procedia Chemistry, ISSN 1876-6196, Vol. 1, no 1, p. 224-227Article in journal (Refereed) Published
    Abstract [en]

    An optically addressable field effect device Incorporating functionalized nano-environments is demonstrated for the generation of multiple response signatures of a same analyte-sensing material combination. The device can operate unsupervised and different response mechanism can be simultaneously tested and incorporated in a multidimensional response. This proof of concept explores the well-known Pd-H-2 case, while the concept is a breadboard for chemical imaging experiments.

    Place, publisher, year, edition, pages
    ELSEVIER SCIENCE BV, 2009
    Keywords
    field effect chemical sensing; chemical images; scanning light pulse technique; suspended gate devices; optical chemical sensors
    National Category
    Engineering and Technology
    Identifiers
    urn:nbn:se:liu:diva-58423 (URN)10.1016/j.proche.2009.07.056 (DOI)000275995600192 ()
    Note

    Proceedings of the Eurosensors XXIII conference.

    Available from: 2010-08-12 Created: 2010-08-11 Last updated: 2015-06-01
    7. HDR imaging evaluation of a NT-proBNP test with a mobile phone.
    Open this publication in new window or tab >>HDR imaging evaluation of a NT-proBNP test with a mobile phone.
    2011 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, no 5, p. 2107-2113Article in journal (Refereed) Published
    Abstract [en]

    The determination of NT-proBNP levels is key for the monitoring of patients with diagnosed heart failure and it is a routine measurement typically performed at health care centers, which would benefit from decentralized alternatives. Here we investigate the quantitative evaluation of a well-established NT-proBNP test using a standard mobile phone (Nokia 6720) as measuring platform rather than a dedicated instrument. A Java ME software developed for this application controls the illumination and imaging of the proBNP test under defined time intervals, which enables the composition of multi-exposure sets that are processed as high dynamic range (HDR) images for contrast enhancement. The results show that HDR processing significantly increases the sensitivity and resolution of the technique achieving a performance within the diagnostics range. These results demonstrate the feasibility to exploit a ubiquitous device to decentralize the evaluation of a routine test and identify key processing alternatives to bring the performance of such systems within the diagnostics range.

    National Category
    Natural Sciences
    Identifiers
    urn:nbn:se:liu:diva-63708 (URN)10.1016/j.bios.2010.09.015 (DOI)000286904400051 ()20926279 (PubMedID)
    Available from: 2010-12-30 Created: 2010-12-30 Last updated: 2017-12-11Bibliographically approved
  • 6.
    Macken, Stephen
    et al.
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
    Di Natale, C.
    Poalesse, R.
    D´Amico, A.
    Lundström, Ingemar
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Filippini, Daniel
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Microstructured devices for computer screen photo-assisted optical fingerprinting of high density response patterns2007In: Transducers ´07 - Eurosensors XXI,2007, 2007Conference paper (Refereed)
  • 7.
    Macken, Stephen
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    Di Natale, Corrado
    University Roma Tor Vergata, Italy.
    Paolesse, Roberto
    University Roma Tor Vergata, Italy.
    DAmico, Arnaldo
    University Roma Tor Vergata, Italy.
    Lundström, Ingemar
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Towards integrated devices for computer screen photo-assisted multi-parameter sensing2009In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 632, no 1, p. 143-147Article in journal (Refereed)
    Abstract [en]

    The computer screen photo-assisted technique (CSPT) utilizes consumer electronic devices such as computer screens and web cameras for distributed chemical sensing. Key to this method is the development of small and disposable sensing assays able to aid the CSPT optical detection, and the evaluation of multiple indicators. Here we demonstrate CSPT identification of fluorescent indicators from individual 100 mu m SU-8 (10) pillars covering an area of 4 mm x 4 mm with a density of 47 elements mm-2. The extraction of distinctive spectral fingerprints is aided by the exploitation of a three-band Moire interference that enables the partial Bayer decoding of the CSPT images.

  • 8.
    Macken, Stephen
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Colour moire interferometry for physical sensing using a standard web camera2010In: Measurement science and technology, ISSN 0957-0233, E-ISSN 1361-6501, Vol. 21, no 10, p. 105305-Article in journal (Refereed)
    Abstract [en]

    Digital moire patterns are an effect seen in digital pictures when a high-contrast periodic object being imaged interferes with the periodic image detector in the camera. This is often an undesired effect, which is usually removed through various filtering processes. However in this work we demonstrate the ability to use a deliberately created moire pattern to measure the physical distance of an object from a web camera with a resolution of 131 mu m. This measurement principle is then adapted to measure weight as a ubiquitous scale with a resolution of 240 mu g.

  • 9.
    Macken, Stephen
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
    Monolithic SU-8 Microcavities for Efficient Fluorescence Collection2009In: Procedia Chemistry, ISSN 1876-6196, Vol. 1, no 1, p. 1115-1118Article in journal (Refereed)
    Abstract [en]

    The fabrication of capped microstructures such as channels or cavities typically involves multiple production steps. In this work we demonstrate a fabrication procedure that enables the generation of capped monolithic microstructures of arbitrary geometry in one single exposure step. The presented method also enables the embedment of metal self-aligned surfaces for use as electrodes or mirrors. The devices furthermore demonstrate a capability of increasing fluorescent collection, as measured by an epifluorescent inverted microscope, by up to 15 fold.

  • 10.
    Macken, Stephen
    et al.
    Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
    Monolithic SU-8 microcavities for efficient fluorescence collection2009In: Journal of Micromechanics and Microengineering, ISSN 0960-1317, E-ISSN 1361-6439, Vol. 19, no 8, p. 085011-Article in journal (Refereed)
    Abstract [en]

    The fabrication of capped microstructures such as channels or cavities typically involves multiple production steps. In this work, we demonstrate a fabrication procedure that enables the generation of capped monolithic microstructures of arbitrary geometry in one single SU-8 exposure step. The presented method also enables the embedment of metal self-aligned surfaces for use as electrodes or mirrors. The devices furthermore demonstrate a capability of increasing fluorescent collection, as measured by an epi-fluorescent inverted microscope, by up to 15-fold.

  • 11.
    Malik, Muhammad Ali
    et al.
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
    Macken, Stephen
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
    Di Natale, C.
    Paolesse, R.
    D´Amico, A.
    Lundström, Ingemar
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Filippini, Daniel
    Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
    Computer screen photo-assisted fingerprinting of the excitation-emission-matrix of fluorescent indicators2007In: ISOEN 2007,2007, 2007Conference paper (Refereed)
  • 12.
    Preechaburana, P.
    et al.
    Thammasat University, Prathum-Thani, Thailand.
    Macken, Stephen
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
    Suska, Anke
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
    Filippini, Daniel
    Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
    HDR imaging evaluation of a NT-proBNP test with a mobile phone.2011In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, no 5, p. 2107-2113Article in journal (Refereed)
    Abstract [en]

    The determination of NT-proBNP levels is key for the monitoring of patients with diagnosed heart failure and it is a routine measurement typically performed at health care centers, which would benefit from decentralized alternatives. Here we investigate the quantitative evaluation of a well-established NT-proBNP test using a standard mobile phone (Nokia 6720) as measuring platform rather than a dedicated instrument. A Java ME software developed for this application controls the illumination and imaging of the proBNP test under defined time intervals, which enables the composition of multi-exposure sets that are processed as high dynamic range (HDR) images for contrast enhancement. The results show that HDR processing significantly increases the sensitivity and resolution of the technique achieving a performance within the diagnostics range. These results demonstrate the feasibility to exploit a ubiquitous device to decentralize the evaluation of a routine test and identify key processing alternatives to bring the performance of such systems within the diagnostics range.

1 - 12 of 12
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