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  • 1.
    Andersson, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Björk, Per
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Herland, Anna
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Tvingstedt, Kristofer
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Biomolecules and conjugated polyelectrolytes in patterning2006Inngår i: NaPa spring meeting 06 Köpenhamn,2006, 2006Konferansepaper (Annet vitenskapelig)
  • 2.
    Andersson, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Björk, Per
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Biomolecules and conjugated polyelectrolytes in patterning2006Inngår i: NaPa fall meeting 06 Glasgow,2006, 2006Konferansepaper (Annet vitenskapelig)
    Abstract [en]

      

  • 3.
    Andersson, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Åsberg, Peter
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Herland, Anna
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Fransson, Sophia
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    von Post, Fredrik
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Conjugated polyelectrolytes as reporter molecules; biochip constructed by Soft litography methods2006Inngår i: ICSM summer 06 Dublin,2006, 2006Konferansepaper (Annet vitenskapelig)
    Abstract [en]

       

  • 4.
    Björk, Per
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Thomsson, Daniel
    Lund University.
    Mirzov, Oleg
    Lund University.
    Andersson, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Scheblykin , Ivan G
    Lund University.
    Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters2009Inngår i: SMALL, ISSN 1613-6810 , Vol. 5, nr 1, s. 96-103Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.

  • 5.
    Hamedi, Mahiar
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Tai, Feng-i
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Björk, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Aili, Daniel
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
    Polypeptide-guided assembly of conducting polymer nanocomposites2010Inngår i: NANOSCALE, ISSN 2040-3364, Vol. 2, nr 10, s. 2058-2061Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    A strategy for fabrication of electroactive nanocomposites with nanoscale organization, based on self-assembly, is reported. Gold nanoparticles are assembled by a polypeptide folding-dependent bridging. The polypeptides are further utilized to recruit and associate with a water soluble conducting polymer. The polymer is homogenously incorporated into the nanocomposite, forming conducting pathways which make the composite material highly conducting.

  • 6.
    Hamedi, Mahiar
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Tai, Feng-I
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Björk, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Aili, Daniel
    Department of Materials and Institute of Bioengineering, Imperial College London, SW7 2AZ London, UK.
    Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites2010Manuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    The development of nanoelectronics has resulted in enormous advancements in fabrication techniques that have enabled massproduction of CMOS circuits with feature sizes below 45nm. There is a large interest in new methods to further push the size limits, lower the production costs and to facilitate the design of more advanced three-dimensional structures beyond today’s 2.5 dimensional architectures. Self-assembly is probably the most important scheme in this development and is currently applied to many different areas and classes of nanoelectronics. Self-assembly enables fabrication of structures well below 10 nm in feature size and allows for incorporation of novel nanomaterials, such as metallic and semiconducting nanoparticles with many interesting optical and electrical properties. The controlled self-assembly of electro-active nanocomposites is of great interest for the development of novel functional materials including biosensors, electrochromic/plasmonic hybrid devices, and polymer/nanoparticle-based memories.

  • 7.
    Hevekerl, Heike
    et al.
    Royal Institute Technology, Sweden .
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Persson, Gustav
    Royal Institute Technology, Sweden .
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Widengren, Jerker
    Royal Institute Technology, Sweden .
    Dark States in Ionic Oligothiophene Bioprobes-Evidence from Fluorescence Correlation Spectroscopy and Dynamic Light Scattering2014Inngår i: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 118, nr 22, s. 5924-5933Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Luminescent conjugated polyelectrolytes (LCPs) can upon interaction with biological macromolecules change their luminescent properties, and thereby serve as conformation- and interaction-sensitive biomolecular probes. However, to exploit this in a more quantitative manner, there is a need to better understand the photophysical processes involved. We report studies of the conjugated pentameric oligothiophene, derivative p-FTAA, which changes optical properties with different p-FTAA concentrations in aqueous buffers, and in a pH and oxygen saturation dependent manner. Using dynamic light scattering, luminescence spectroscopy and fluorescence correlation spectroscopy, we find evidence for a monomer dimer equilibrium, for the formation of large clusters of p-FTAA in aqueous environment, and can couple aggregation to changed emission properties of oligothiophenes. In addition, we observe the presence of at least two dark transient states, one presumably being a triplet state. Oxygen was found to statically quench the p-FTAA fluorescence but also to promote molecular fluorescence by quenching dark transient states of the p-FTAA molecules. Taken together, this study provides knowledge of fluorescence and photophysical features essential for applying p-FTAA and other oligothiophene derivatives for diagnostic purposes, including detection and staining of amyloid aggregates.

  • 8.
    Karlsson, Roger
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.
    Herland, Anna
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Hamedi, Mahiar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Åslund, Andreas
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.
    Liu, Xianjie
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska högskolan.
    Fahlman, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska högskolan.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Konradsson, Peter
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.
    Iron-Catalyzed Polymerization of Alkoxysulfonate-Functionalized 3,4-Ethylenedioxythiophene Gives Water-Soluble Poly(3,4-ethylenedioxythiophene) of High Conductivity2009Inngår i: Chemistry of Materials, ISSN 0897-4756, E-ISSN 1520-5002, Vol. 21, nr 9, s. 1815-1821Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Chemical polymerization of a 3,4-ethylenedioxythiophene derivative bearing a sulfonate group (EDOTS) is reported. The polymer, PEDOT-S, is fully water-soluble and has been produced by polymerizing EDOT-S in water, using Na2S2O8 and a catalytic amount of FeCl3. Elemental analysis and XPS measurements indicate that PEDOT-S is a material with a substantial degree of self-doping, but also contains free sulfate ions as charge-balancing counterions of the oxidized polymer. Apart from self-doping PEDOT-S, the side chains enable full water solubility of the material; DLS studies show an average cluster size of only 2 nm. Importantly, the solvation properties of the PEDOT-S are reflected in spin-coated films, which show a surface roughness of 1.2 nm and good conductivity (12 S/cm) in ambient conditions. The electro-optical properties of this material are shown with cyclic voltammetry and spectroelectrochemical experiment reveals an electrochromic contrast (similar to 48% at lambda(max) = 606 nm).

  • 9.
    Magnusson, Karin
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska högskolan.
    Appelqvist, Hanna
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska högskolan.
    Cieslar-Pobuda, Artur
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet. Institute of Automatic Control, Silesian University of of TechnologyGliwice, Poland.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan. Carl Zeiss AB, Sweden.
    Karlsson, Thommie
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Hälsouniversitetet. Application Specialist Confocal Microscopy at Leica MicrosystemsIL, United States.
    Los, Marek Jan
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet. Department of Pathology, Pomeranian Medical UniversitySzczecin, Poland.
    Kågedal, Bertil
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Hälsouniversitetet. Östergötlands Läns Landsting, Diagnostikcentrum, Klinisk kemi.
    Jonasson, Jon
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Region Östergötland, Diagnostikcentrum, Klinisk patologi och klinisk genetik.
    Nilsson, Peter
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
    Differential vital staining of normal fibroblasts and melanoma cells by an anionic conjugated polyelectrolyte2015Inngår i: Cytometry Part A, ISSN 1552-4922, E-ISSN 1552-4930, Vol. 87, nr 3, s. 262-272Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Molecular probes for imaging of live cells are of great interest for studying biological and pathological processes. The anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA), has previously been used for vital staining of cultured fibroblasts as well as transformed cells with results indicating differential staining due to cell phenotype. Herein, we investigated the behavior of PTAA in two normal and five transformed cells lines. PTAA fluorescence in normal cells appeared in a peripheral punctated pattern whereas the probe was more concentrated in a one-sided perinuclear localization in the five transformed cell lines. In fibroblasts, PTAA fluorescence was initially associated with fibronectin and after 24 h partially localized to lysosomes. The uptake and intracellular target in malignant melanoma cells was more ambiguous and the intracellular target of PTAA in melanoma cells is still elusive. PTAA was well tolerated by both fibroblasts and melanoma cells, and microscopic analysis as well as viability assays showed no signs of negative influence on growth. Stained cells maintained their proliferation rate for at least 12 generations. Although the probe itself was nontoxic, photoinduced cellular toxicity was observed in both cell lines upon irradiation directly after staining. However, no cytotoxicity was detected when the cells were irradiated 24 h after staining, indicating that the photoinduced toxicity is dependent on the cellular location of the probe. Overall, these studies certified PTAA as a useful agent for vital staining of cells, and that PTAA can potentially be used to study cancer-related biological and pathological processes.

  • 10.
    Teixeira, A.I.
    et al.
    Karolinska Institute.
    Ilkhanizadeh, S.
    Karolinska Institute.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Duckworth, J.K.
    Karolinska Institute.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Hermanson, O.
    Karolinska Institute.
    The promotion of neuronal maturation on soft substrates2009Inngår i: Biomaterials, ISSN 0142-9612, Vol. 30, nr 27, s. 4567-4572Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Microenvironmental mechanical properties of stem cell niches vary across tissues and developmental stages. Accumulating evidence suggests that matching substrate elasticity with in vivo tissue elasticity facilitates stem cell differentiation. However, it has not been established whether substrate elasticity can control the maturation stage of cells generated by stem cell differentiation. Here we show that soft substrates with elasticities commensurable to the elasticity of the brain promote the maturation of neural stem cell-derived neurons. In the absence of added growth factors, neurons differentiated on soft substrates displayed long neurites and presynaptic terminals, contrasting with the bipolar immature morphology of neurons differentiated on stiff substrates. Further, soft substrates supported an increase in astrocytic differentiation. However, stiffness cues could not override the dependency of astrocytic differentiation on Notch signaling. These results demonstrate that substrate elasticity per se can drive neuronal maturation thus defining a crucial parameter in neuronal differentiation of stem cells.

  • 11.
    Wigenius, Jens
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

    Delarbeid
    1. Limits to Nanopatterning of Fluids on Surfaces in Soft Lithography
    Åpne denne publikasjonen i ny fane eller vindu >>Limits to Nanopatterning of Fluids on Surfaces in Soft Lithography
    2008 (engelsk)Inngår i: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 18, nr 17, s. 2563-2571Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Soft lithographic microcontact printing using the residual polydimethylsiloxane (PDMS) found in elastomeric PDMS stamps is demonstrated to lead to unstable prints with sub-micrometer dimensions. The statics and dynamics of the process have been followed with time-resolved atomic force microscopy, imaging ellipsometry, water contact angle measurement, and optical diffraction. It is proposed that this instability places a fundamental limitation on patterning by macromolecular fluids, which is of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

    Emneord
    Lithography, microcontact printing (µCP), nanostructures, nanowires, surface patterning
    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-42810 (URN)10.1002/adfm.200800073 (DOI)68960 (Lokal ID)68960 (Arkivnummer)68960 (OAI)
    Tilgjengelig fra: 2009-10-10 Laget: 2009-10-10 Sist oppdatert: 2017-12-13
    2. Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
    Åpne denne publikasjonen i ny fane eller vindu >>Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
    2008 (engelsk)Inngår i: BIOINTERPHASES, ISSN 1559-4106 , Vol. 3, nr 3, s. 75-82Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly (dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain.

    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-17907 (URN)10.1116/1.2988771 (DOI)
    Tilgjengelig fra: 2009-04-26 Laget: 2009-04-24 Sist oppdatert: 2010-04-20
    3. DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode
    Åpne denne publikasjonen i ny fane eller vindu >>DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode
    Vise andre…
    2010 (engelsk)Inngår i: LANGMUIR, ISSN 0743-7463, Vol. 26, nr 5, s. 3753-3759Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    We show how to use well-defined conjugated polyelectrolytes (CPEs) combined With Surface energy patterning to Fabricate DNA Chips utilizing A fluorescence signal amplification. Cholesterol-modified DNA strands in complex with it CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with it short complementary strand from Solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.

    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-54255 (URN)10.1021/la903101v (DOI)000274636900113 ()
    Tilgjengelig fra: 2010-03-05 Laget: 2010-03-05 Sist oppdatert: 2015-05-29
    4. Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters
    Åpne denne publikasjonen i ny fane eller vindu >>Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters
    Vise andre…
    2009 (engelsk)Inngår i: SMALL, ISSN 1613-6810 , Vol. 5, nr 1, s. 96-103Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.

    Emneord
    aggregation, conjugated polymers, DNA, fluorescence, single-molecule spectroscopy
    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-16829 (URN)10.1002/smll.200800855 (DOI)
    Tilgjengelig fra: 2009-02-20 Laget: 2009-02-20 Sist oppdatert: 2010-04-20
    5. Dark states in oligothiophenes: evidence from fluorescence correlation spectroscopy and dynamic light scattering
    Åpne denne publikasjonen i ny fane eller vindu >>Dark states in oligothiophenes: evidence from fluorescence correlation spectroscopy and dynamic light scattering
    Vise andre…
    (engelsk)Manuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    We report studies of the conjugated pentameric oligothiophene derivative p-FTAA, which changes optical properties in aqueous buffers of varying pH and concentration. Using dynamic light scattering, luminescence spectroscopy and fluorescence correlation spectroscopy, we find evidence for the formation of large clusters of p-FTAA in aqueous environment, formation of very large non-emissive clusters, and the presence of at least two dark transient states, one presumably being a triplet state. The clustering of p-FTAA is therefore an important mechanism. This work provides an interpretation of fluorescence spectra used for the detection of misfolding proteins through interaction with p-FTAA.

    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-54897 (URN)
    Tilgjengelig fra: 2010-04-20 Laget: 2010-04-20 Sist oppdatert: 2014-04-08
    6. Interactions between a luminescent conjugated oligoelectrolyte and insulin during early phases of amyloid formation
    Åpne denne publikasjonen i ny fane eller vindu >>Interactions between a luminescent conjugated oligoelectrolyte and insulin during early phases of amyloid formation
    2011 (engelsk)Inngår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 11, nr 8, s. 1120-1127Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Folding of an amino acid polypeptide chain into its native three-dimensional protein is a delicate process. Misfolding may cause assembly of dysfunctional proteins leading to aggregated assemblies, in medicine denoted amyloids, causing Alzheimer’s, Parkinson and a number of other protein related diseases. Amyloids have also shown promising results as building blocks in organic electronic applications, associated to conjugated polymers. Luminescent conjugated oligo- and polythiophenes (LCPs) have been further developed for biosensor applications exhibiting good ability to discriminate and determine different types of amyloid enrichment in complex environments, such as in tissue sections. The nature of interaction between the amyloid assemblies and LCPs is still not fully understood. In this study we use steady-state fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy and fluorescence correlation spectroscopy to follow the interplay between the anionic oligothiophene derivative 4',3'''-Bis-carboxymethyl-[2,2';5',2'';5'',2''';5''',2'''']quinque thiophene-5,5''''-dicarboxylic acid (p-FTAA), and prefibrillar protein assemblies present during the earlier stage of in vitro fibrillation of bovine insulin. Our findings confirm that p-FTAA interacts with pre-fibrillar species of insulin preceding the formation of mature insulin amyloid fibrils, and insights regarding the molecular interplay between p-FTAA and these species are provided.

    sted, utgiver, år, opplag, sider
    Wiley, 2011
    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-54898 (URN)10.1002/mabi.201100016 (DOI)000294160900011 ()
    Merknad
    Funding Agencies|Swedish Science Council (VR)||Strategic Research Foundation (SSF) through the center for organic bioelectronics (OBOE)||Knut and Alice Wallenberg foundation||Tilgjengelig fra: 2010-04-20 Laget: 2010-04-20 Sist oppdatert: 2017-12-12
    7. Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes
    Åpne denne publikasjonen i ny fane eller vindu >>Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes
    2010 (engelsk)Inngår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 10, nr 8, s. 836-841Artikkel i tidsskrift (Fagfellevurdert) Published
    Abstract [en]

    Designed polypeptides with controllable folding properties are utilized as supramolecular templates for fabrication of ordered nanoscale molecular and fibrous assemblies of luminescent conjugated polymers (LCPs). The properties of the LCPs as well as the three dimensional conformation of the polypeptide-scaffold determine how the polymers are arranged in the supramolecular construct, which highly affects the properties of the hybrid material. The ability to control the polypeptide conformation and assembly into fibers provide a promising route for tuning the optical properties of LCPs and for fabrication of complex functional supramolecules with well defined structural properties.

    sted, utgiver, år, opplag, sider
    John Wiley & Sons, 2010
    Emneord
    α-helical; conjugated polymers; hybrid materials; peptides; self-assembly
    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-54899 (URN)10.1002/mabi.200900463 (DOI)000281387900002 ()
    Tilgjengelig fra: 2010-04-20 Laget: 2010-04-20 Sist oppdatert: 2017-12-12bibliografisk kontrollert
    8. Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites
    Åpne denne publikasjonen i ny fane eller vindu >>Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites
    Vise andre…
    2010 (engelsk)Manuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    The development of nanoelectronics has resulted in enormous advancements in fabrication techniques that have enabled massproduction of CMOS circuits with feature sizes below 45nm. There is a large interest in new methods to further push the size limits, lower the production costs and to facilitate the design of more advanced three-dimensional structures beyond today’s 2.5 dimensional architectures. Self-assembly is probably the most important scheme in this development and is currently applied to many different areas and classes of nanoelectronics. Self-assembly enables fabrication of structures well below 10 nm in feature size and allows for incorporation of novel nanomaterials, such as metallic and semiconducting nanoparticles with many interesting optical and electrical properties. The controlled self-assembly of electro-active nanocomposites is of great interest for the development of novel functional materials including biosensors, electrochromic/plasmonic hybrid devices, and polymer/nanoparticle-based memories.

    Emneord
    Conducting Polymers, Organic Electronics, Conducting Nanowires, Self- Assembly, Supramolecular Materials
    HSV kategori
    Identifikatorer
    urn:nbn:se:liu:diva-54901 (URN)
    Tilgjengelig fra: 2010-04-20 Laget: 2010-04-20 Sist oppdatert: 2010-04-20
  • 12.
    Wigenius, Jens A.
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Persson, Gustav
    Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
    Nilsson, Peter
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Organisk Kemi. Linköpings universitet, Tekniska högskolan.
    Widengren, Jerker
    Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Dark states in oligothiophenes: evidence from fluorescence correlation spectroscopy and dynamic light scatteringManuskript (preprint) (Annet vitenskapelig)
    Abstract [en]

    We report studies of the conjugated pentameric oligothiophene derivative p-FTAA, which changes optical properties in aqueous buffers of varying pH and concentration. Using dynamic light scattering, luminescence spectroscopy and fluorescence correlation spectroscopy, we find evidence for the formation of large clusters of p-FTAA in aqueous environment, formation of very large non-emissive clusters, and the presence of at least two dark transient states, one presumably being a triplet state. The clustering of p-FTAA is therefore an important mechanism. This work provides an interpretation of fluorescence spectra used for the detection of misfolding proteins through interaction with p-FTAA.

  • 13.
    Wigenius, Jens A.
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Persson, Gustav
    Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
    Widengren, Jerker
    Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Interactions between a luminescent conjugated oligoelectrolyte and insulin during early phases of amyloid formation2011Inngår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 11, nr 8, s. 1120-1127Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Folding of an amino acid polypeptide chain into its native three-dimensional protein is a delicate process. Misfolding may cause assembly of dysfunctional proteins leading to aggregated assemblies, in medicine denoted amyloids, causing Alzheimer’s, Parkinson and a number of other protein related diseases. Amyloids have also shown promising results as building blocks in organic electronic applications, associated to conjugated polymers. Luminescent conjugated oligo- and polythiophenes (LCPs) have been further developed for biosensor applications exhibiting good ability to discriminate and determine different types of amyloid enrichment in complex environments, such as in tissue sections. The nature of interaction between the amyloid assemblies and LCPs is still not fully understood. In this study we use steady-state fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy and fluorescence correlation spectroscopy to follow the interplay between the anionic oligothiophene derivative 4',3'''-Bis-carboxymethyl-[2,2';5',2'';5'',2''';5''',2'''']quinque thiophene-5,5''''-dicarboxylic acid (p-FTAA), and prefibrillar protein assemblies present during the earlier stage of in vitro fibrillation of bovine insulin. Our findings confirm that p-FTAA interacts with pre-fibrillar species of insulin preceding the formation of mature insulin amyloid fibrils, and insights regarding the molecular interplay between p-FTAA and these species are provided.

  • 14.
    Wigenius, Jens
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Björk, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Hamedi, Mahiar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Aili, Daniel
    Department of Materials, Imperial College London, SW7 2AZ London, UK.
    Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes2010Inngår i: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 10, nr 8, s. 836-841Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Designed polypeptides with controllable folding properties are utilized as supramolecular templates for fabrication of ordered nanoscale molecular and fibrous assemblies of luminescent conjugated polymers (LCPs). The properties of the LCPs as well as the three dimensional conformation of the polypeptide-scaffold determine how the polymers are arranged in the supramolecular construct, which highly affects the properties of the hybrid material. The ability to control the polypeptide conformation and assembly into fibers provide a promising route for tuning the optical properties of LCPs and for fabrication of complex functional supramolecules with well defined structural properties.

  • 15.
    Wigenius, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Fransson, Sophia
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Conjugated Polyelectrolytes as Reporter Molecules2007Inngår i: E-MRS 2007 Strasbourg,2007, 2007Konferansepaper (Fagfellevurdert)
  • 16.
    Wigenius, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Fransson, Sophia
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Conjugated Polyelectrolytes as Reporter Molecules;Biochip Constructed by Soft Litography Method2007Inngår i: BIOSCOPE 2007,2007, 2007Konferansepaper (Annet vitenskapelig)
    Abstract [en]

       

  • 17.
    Wigenius, Jens
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Fransson, Sophia
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    von Post, Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Inganäs , Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes2008Inngår i: BIOINTERPHASES, ISSN 1559-4106 , Vol. 3, nr 3, s. 75-82Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly (dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain.

  • 18.
    Wigenius, Jens
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Hamedi, Mahiar
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Limits to Nanopatterning of Fluids on Surfaces in Soft Lithography2008Inngår i: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 18, nr 17, s. 2563-2571Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Soft lithographic microcontact printing using the residual polydimethylsiloxane (PDMS) found in elastomeric PDMS stamps is demonstrated to lead to unstable prints with sub-micrometer dimensions. The statics and dynamics of the process have been followed with time-resolved atomic force microscopy, imaging ellipsometry, water contact angle measurement, and optical diffraction. It is proposed that this instability places a fundamental limitation on patterning by macromolecular fluids, which is of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

  • 19.
    Wigenius, Jens
    et al.
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Inganäs, Olle
    Linköpings universitet, Tekniska högskolan. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik.
    Limits to Nanopatterning of Fluids on Surfaces2007Inngår i: E-MRS Strasbourg 2007,2007, 2007Konferansepaper (Annet vitenskapelig)
  • 20.
    Wigenius, Jens
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Magnusson, Karin
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
    Björk, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    Andersson, Olof
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
    Inganäs, Olle
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biomolekylär och Organisk Elektronik. Linköpings universitet, Tekniska högskolan.
    DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode2010Inngår i: LANGMUIR, ISSN 0743-7463, Vol. 26, nr 5, s. 3753-3759Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We show how to use well-defined conjugated polyelectrolytes (CPEs) combined With Surface energy patterning to Fabricate DNA Chips utilizing A fluorescence signal amplification. Cholesterol-modified DNA strands in complex with it CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with it short complementary strand from Solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.

1 - 20 of 20
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