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  • 301.
    Carlsson, Staffan
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Skillnader i mulmvolymer mellan fem trädslag i Östergötlands eklandskap2013Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Gamla hålträd av ek hyser ofta en stor mängd mulm. Tyvärr har dessa träd det senaste århundradet minskat i antal. Det har fått till följd att arter som är beroende av dessa habitat riskerar att minska eller dö ut regionalt. Tidigare studier har visat att volymen av mulm i ett träd är en viktig faktor för förekomst och populationsstorlek. Syftet med denna studie var att undersöka hur mulmvolymer varierar mellan olika trädslag. Mulmvolymen mättes därför hos ask (

    Fraxinus excelsior), lind (Tilia sp.), lönn (Acer platanoides), asp (Populus tremula) och ek (Quercus robur). På varje träd mättes mulmdjup, invändig håldiameter, invändig hålhöjd, omkrets samt om trädet hade vitröta eller brunröta. Sammanlagt mättes 23 askar, 20 lindar, 24 lönnar, 24 aspar och 21 ekar runt Motala (Östergötland) med omnejd. Resultaten visar att det finns signifikanta skillnader mellan trädslagen gällande mulmvolymer. Eken hyser större volymer än de övriga, men ask och lönn har även de relativt stora volymer mulm. Asken har dock större volym mulm än vad eken har vid samma omkrets. Studien visar att man vid naturvårdsplaneringar bör ta med ask som ett komplement till eken i ett fragmenterat landskap. Eftersom asken kan generera större volymer mulm än eken vid samma omkrets medför detta möjligheten att asken kan fungera som habitat i ett tidigare skede. I isolerade fragment av landskapet kan då chansen för överlevnad möjligtvis öka för de arter som är beroende av mulmhabitat.

  • 302.
    Carlsson, Staffan
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Bergman, Karl-Olof
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Jansson, Nicklas
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Ranius, Thomas
    Department of Ecology, Swedish University of Agricultural Sciences, Box 7044, 750 07 Uppsala, Sweden.
    Milberg, Per
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Ekologi. Linköpings universitet, Tekniska fakulteten.
    Boxing for biodiversity: evaluation of an artificiallycreated decaying wood habitat2016Ingår i: Biodiversity and Conservation, ISSN 0960-3115, E-ISSN 1572-9710, Vol. 25, nr 2, s. 393-405Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Many saproxylic species are threatened in Europe because of habitat decline.Hollow trees represent an important habitat for saproxylic species. Artificial habitats mayneed to be created to maintain or increase the amount of habitat due to natural habitat decline.This study investigated the extent to which saproxylic beetles use artificial habitats in woodenboxes. The boxes were placed at various distances (0–1800 m) from known biodiversityhotspots with hollow oaks and studied over 10 years. Boxes were mainly filled with oak sawdust, oak leaves, hay and lucerne flour. In total, 2170 specimens of 91 saproxylic beetlespecies were sampled in 43 boxes. The abundance of species associated with tree hollows,wood rot and animal nests increased from the fourth to the final year, but species richnessdeclined for all groups. This study shows that wooden boxes can function as saproxylicspecies habitats. The artificial habitats developed into a more hollow-like environment duringthe decade long experiment with fewer but more abundant tree hollow specialists.

  • 303.
    Carrasco Del Amor, Ana Maria
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Freitas, Sara
    Interdisciplinary Ctr Marine and Environm Res, Portugal.
    Urbatzka, Ralph
    Interdisciplinary Ctr Marine and Environm Res, Portugal.
    Fresnedo, Olatz
    Univ Basque Country, Spain.
    Cristobal, Susana
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten. Univ Basque Country, Spain.
    Application of Bioactive Thermal Proteome Profiling to Decipher the Mechanism of Action of the Lipid Lowering 13(2)-Hydroxy-pheophytin Isolated from a Marine Cyanobacteria2019Ingår i: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 17, nr 6Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The acceleration of the process of understanding the pharmacological application of new marine bioactive compounds requires identifying the compound protein targets leading the molecular mechanisms in a living cell. The thermal proteome profiling (TPP) methodology does not fulfill the requirements for its application to any bioactive compound lacking chemical and functional characterization. Here, we present a modified method that we called bTPP for bioactive thermal proteome profiling that guarantees target specificity from a soluble subproteome. We showed that the precipitation of the microsomal fraction before the thermal shift assay is crucial to accurately calculate the melting points of the protein targets. As a probe of concept, the protein targets of 13(2)-hydroxy-pheophytin, a compound previously isolated from a marine cyanobacteria for its lipid reducing activity, were analyzed on the hepatic cell line HepG2. Our improved method identified 9 protein targets out of 2500 proteins, including 3 targets (isocitrate dehydrogenase, aldehyde dehydrogenase, phosphoserine aminotransferase) that could be related to obesity and diabetes, as they are involved in the regulation of insulin sensitivity and energy metabolism. This study demonstrated that the bTPP method can accelerate the field of biodiscovery, revealing protein targets involved in mechanisms of action (MOA) connected with future applications of bioactive compounds.

  • 304.
    Cassens, U.
    et al.
    Institute of Transfusion Medicine, University of Münster, D-48149 Münster, Germany.
    Lewinski, G.
    Unit of Surgery, Municipal Hospital, 38-300 Gorlice, Poland.
    Samraj, A. K.
    Institute of Molecular Medicine, University of Düsseldorf, D-40225 Düsseldorf, Germany.
    von Bernuth, H.
    Children´s University Clinic, Laboratory for Clinical Research, D-01307 Dresden, Germany.
    Baust, H.
    Department of Radiotherapy, University of Ulm, D-89070 Ulm, Germany.
    Khazaie, K.
    Department of Cancer Immunology and Aids, Dana Farber Cancer Institute/Harvard Medical School, Boston, Massachusetts, MA 02115, USA.
    Los, Marek Jan
    Institute of Experimental Dermatology, University of Muenster, Germany.
    Viral modulation of cell death by inhibition of caspases2003Ingår i: Archivum Immunologiae et Therapiae Experimentalis, ISSN 0004-069X, E-ISSN 1661-4917, Vol. 51, nr 1, s. 19-27Artikel, forskningsöversikt (Refereegranskat)
    Abstract [en]

    Caspases are key effectors of the apoptotic process. Some of them play important roles in the immune system, being involved in the proteolytic maturation of the key cytokines, including interleukin 1beta (IL-1beta) and IL-18. The latter directs the production of interferon gamma (IFN-gamma). Among pathogens, particularly viruses express various modulators of caspases that inhibit their activity by direct binding. By evading the apoptotic process, viruses can better control their production in the infected cell and avoid the attack of the immune system. Targeting the maturation of the key cytokines involved in the initiation of (antiviral) immune response helps to avoid recognition and eradication by the immune system. The three main classes of caspase inhibitors frequently found among viruses include serine proteinase inhibitors (serpins: CrmA/SPI-2), viral IAPs (vIAPs) and p35. Their molecular mechanisms of action, structures and overall influence on cellular physiology are discussed in the review below.

  • 305.
    Castells-Nobau, Anna
    et al.
    Radboud University of Nijmegen, Netherlands.
    Nijhof, Bonnie
    Radboud University of Nijmegen, Netherlands.
    Eidhof, Ilse
    Radboud University of Nijmegen, Netherlands.
    Wolf, Louis
    Radboud University of Nijmegen, Netherlands.
    Scheffer-de Gooyert, Jolanda M.
    Radboud University of Nijmegen, Netherlands.
    Monedero Cobeta, Ignacio
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Medicinska fakulteten. University of Autonoma Madrid, Spain.
    Torroja, Laura
    University of Autonoma Madrid, Spain.
    van der Laak, Jeroen A. W. M.
    Radboud University of Nijmegen, Netherlands; Radboud University of Nijmegen, Netherlands.
    Schenck, Annette
    Radboud University of Nijmegen, Netherlands.
    Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology2017Ingår i: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, nr 123, artikel-id e55395Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Synaptic morphology is tightly related to synaptic efficacy, and in many cases morphological synapse defects ultimately lead to synaptic malfunction. The Drosophila larval neuromuscular junction (NMJ), a well-established model for glutamatergic synapses, has been extensively studied for decades. Identification of mutations causing NMJ morphological defects revealed a repertoire of genes that regulate synapse development and function. Many of these were identified in large-scale studies that focused on qualitative approaches to detect morphological abnormalities of the Drosophila NMJ. A drawback of qualitative analyses is that many subtle players contributing to NMJ morphology likely remain unnoticed. Whereas quantitative analyses are required to detect the subtler morphological differences, such analyses are not yet commonly performed because they are laborious. This protocol describes in detail two image analysis algorithms Drosophila NMJ Morphometrics and Drosophila NMJ Bouton Morphometrics, available as Fiji-compatible macros, for quantitative, accurate and objective morphometric analysis of the Drosophila NMJ. This methodology is developed to analyze NMJ terminals immunolabeled with the commonly used markers Dlg-1 and Brp. Additionally, its wider application to other markers such as Hrp, Csp and Syt is presented in this protocol. The macros are able to assess nine morphological NMJ features: NMJ area, NMJ perimeter, number of boutons, NMJ length, NMJ longest branch length, number of islands, number of branches, number of branching points and number of active zones in the NMJ terminal.

  • 306.
    Cauchoix, M.
    et al.
    CNRS, France; Inst Adv Study Toulouse, France.
    Chow, P. K. Y.
    Univ Exeter, England; Hokkaido Univ, Japan.
    van Horik, J. O.
    Univ Exeter, England.
    Atance, C. M.
    Univ Ottawa, Canada.
    Barbeau, E. J.
    UPS, France.
    Barragan-Jason, G.
    Inst Adv Study Toulouse, France.
    Bize, P.
    Univ Aberdeen, Scotland.
    Boussard, A.
    Stockholm Univ, Sweden.
    Buechel, S. D.
    Stockholm Univ, Sweden.
    Cabirol, A.
    Univ Paul Sabatier, France.
    Cauchard, L.
    Univ Montreal, Canada.
    Claidiere, N.
    Aix Marseille Univ, France.
    Dalesman, S.
    Aberystwyth Univ, Wales.
    Devaud, J. M.
    Univ Paul Sabatier, France.
    Didic, M.
    AP HM Timone, France; Inst Neurosci Syst, France.
    Doligez, B.
    Univ Lyon 1, France.
    Fagot, J.
    Aix Marseille Univ, France.
    Fichtel, C.
    German Primate Ctr, Germany; Univ Gottingen, Germany; Leibniz Sci Campus Primate Cognit, Germany.
    Henke-von der Malsburg, J.
    German Primate Ctr, Germany; Univ Gottingen, Germany; Leibniz Sci Campus Primate Cognit, Germany.
    Hermer, E.
    Univ Gottingen, Germany.
    Huber, L.
    Leibniz Sci Campus Primate Cognit, Germany.
    Huebner, F.
    German Primate Ctr, Germany; Univ Gottingen, Germany; Leibniz Sci Campus Primate Cognit, Germany.
    Kappeler, P. M.
    German Primate Ctr, Germany; Univ Gottingen, Germany; Leibniz Sci Campus Primate Cognit, Germany.
    Klein, S.
    Univ Paul Sabatier, France.
    Langbein, J.
    Leibniz Inst Farm Anim Biol, Germany.
    Langley, E. J. G.
    Univ Exeter, England.
    Lea, S. E. G.
    Univ Exeter, England.
    Lihoreau, M.
    Univ Paul Sabatier, France.
    Lovlie, Hanne
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Matzel, L. D.
    Rutgers State Univ, NJ USA.
    Nakagawa, S.
    Univ New South Wales, Australia; Univ New South Wales, Australia.
    Nawroth, C.
    Leibniz Inst Farm Anim Biol, Germany.
    Oesterwind, S.
    Univ Rostock, Germany.
    Sauce, B.
    Rutgers State Univ, NJ USA.
    Smith, E. A.
    Univ Lincoln, England.
    Sorato, Enrico
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Tebbich, S.
    Univ Vienna, Austria.
    Wallis, L. J.
    Univ Vienna, Austria; Eotvos Lorand Univ, Hungary.
    Whiteside, M. A.
    Univ Exeter, England.
    Wilkinson, A.
    Univ Lincoln, England.
    Chaine, A. S.
    CNRS, France; Inst Adv Study Toulouse, France.
    Morand-Ferron, J.
    Univ Ottawa, Canada.
    The repeatability of cognitive performance: a meta-analysis2018Ingår i: Philosophical Transactions of the Royal Society of London. Biological Sciences, ISSN 0962-8436, E-ISSN 1471-2970, Vol. 373, nr 1756, artikel-id 20170281Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Behavioural and cognitive processes play important roles in mediating an individuals interactions with its environment. Yet, while there is a vast literature on repeatable individual differences in behaviour, relatively little is known about the repeatability of cognitive performance. To further our understanding of the evolution of cognition, we gathered 44 studies on individual performance of 25 species across six animal classes and used meta-analysis to assess whether cognitive performance is repeatable. We compared repeatability (R) in performance (1) on the same task presented at different times (temporal repeatability), and (2) on different tasks that measured the same putative cognitive ability (contextual repeatability). We also addressed whether R estimates were influenced by seven extrinsic factors (moderators): type of cognitive performance measurement, type of cognitive task, delay between tests, origin of the subjects, experimental context, taxonomic class and publication status. We found support for both temporal and contextual repeatability of cognitive performance, with mean R estimates ranging between 0.15 and 0.28. Repeatability estimates were mostly influenced by the type of cognitive performance measures and publication status. Our findings highlight the widespread occurrence of consistent inter-individual variation in cognition across a range of taxa which, like behaviour, may be associated with fitness outcomes. This article is part of the theme issue Causes and consequences of individual differences in cognitive abilities.

  • 307.
    Cavanillas, Santiago
    et al.
    University of Barcelona, Spain.
    Winquist, Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska högskolan.
    Eriksson, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemiska och optiska sensorsystem. Linköpings universitet, Tekniska högskolan.
    A self-polishing platinum ring voltammetric sensor and its application to complex media2015Ingår i: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 859, s. 29-36Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A self-polishing voltammetric sensor was recently developed and has been applied to samples of urea, milk and sewage water. The polishing device continuously grinds a platinum ring electrode, offering a reproducible and clean electrode surface. Principal component analysis (PCA) and partial least squares (PLS) techniques were applied to interpret the data and to build prediction models. In an evaluation of samples with different urea concentrations, the grinding step allows for repeatable measurements, similar to those after electrochemical cleaning. Furthermore, for the determination of sewage water concentrations in drinking water and for the evaluation of different fat contents in milk samples, the polishing eliminates sensor drift produced by electrode fouling. The results show that the application of a self-polishing unit offers a promising tool for electrochemical studies of difficult analytes and complex media. (C) 2014 Elsevier B.V. All rights reserved.

  • 308.
    Cederberg, Lisa
    Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Felaktig alternativ splicing: Vissa mutationer i BRCA1, BRCA2, ERα och ERβ är starkt förknippade med bröstcancer2011Självständigt arbete på grundnivå (kandidatexamen), 10,5 poäng / 16 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Alternative splicing is a process that partly rejects the common definition of a gene – that one gene codes for one specific protein. By variable combination of coding regions (exons) and exclusion of non-coding regions (introns), formation of several different mRNA-transcripts, and consequently several different proteins, can derive from the same gene. Alternative splicing is an important condition for the development of complex life forms, but it is also a highly sensitive process and inaccurate splicing is the cause of approximately 15 % of mutations that cause genetic diseases. This article presents four genes, BRCA1, BRCA2, ERα and ERβ, and inaccurate splicing of these genes increases the risk of developing cancer, particularly breast cancer and ovarian cancer. Breast cancer is the second most common form of lethal cancer among women. After identifying the cancerogenic mutations, women of high-risk families can undergo genetic testing and preventive therapy can reduce the morbidity and mortality. The article also presents a short discussion around the ethical problems of genetic testing, and the social and psychological dilemmas women of high-risk families are facing when they are given the option to undergo genetic testing.

  • 309.
    Cederlund, Joakim
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi.
    Behavioural responses of Amur tigers (Panthera tigris altaica) and African lions (Panthera leo) to conspecific urine and to a component of tiger marking fluid2018Självständigt arbete på avancerad nivå (masterexamen), 40 poäng / 60 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Olfactory signals are an important means of social communication among felids. However, not much is known about how individual volatiles of body-borne odours influence behavioural responses. 2-acetyl-1pyrroline has recently been identified as a characteristic component of tiger marking fluid, while being absent from lion marking fluid. One pride each of captive Amur tigers (Panthera tigris altaica) and African lions (Panthera leo) were presented with wooden logs impregnated with four different odours and their behaviour was observed. The tigers displayed significantly more interactions towards the marking fluid component (2-acetyl-1-pyrroline), the conspecific urine odour, and the fruity odour (iso-pentyl acetate) than towards the near odourless control (diethyl phthalate). The lions displayed significantly more behaviours towards conspecific urine than towards any of the other odours.  In general all lions interacted more with the logs than tigers. Hence, these results support the notion that 2-acetyl-1-pyrroline is a species-specific odorant for tiger olfactory communication. Furthermore, the results show that a single compound (2-acetyl-1pyrroline) can elicit behavioural responses to the same degree as a complex chemical mixture (tiger urine). The high number of interactions performed by both species towards the wooden logs impregnated with conspecific urine suggests that conspecific odours are suitable to use as olfactory enrichment for captive felids.

  • 310.
    Cederlund, Joakim
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi.
    Eye preference in humans and its correlation with eye dominance, visual acuity and handedness2016Självständigt arbete på grundnivå (kandidatexamen), 10,5 poäng / 16 hpStudentuppsats (Examensarbete)
    Abstract [en]

    Handedness is the most obvious expression of lateralized behaviour in humans. However, there is only limited knowledge about other forms of lateralized behaviour, e.g. preferential use of an eye and whether these may correlate with handedness. Thus to investigate this, 100 subjects (50 males and 50 females) between 11 and 80 years of age were assessed for their eye preference, eye dominance, visual acuity, and handedness. Eye preference was assessed by performing four different monocular tasks, eye dominance by performing the binocular Dolman test, visual acuity was assessed with a Snellen chart and handedness was surveyed using the Edinburgh Handedness Inventory. Regarding eye preference, the right eye was preferred by 69% of the subjects. 90 % of the subjects were consistent for their preferred eye across all four tasks. 66% of the subjects had a dominant right eye, 33% had left eye dominance and 1% could not be assessed using the Dolman test. 56% of the subjects differed in their visual acuity between both eyes, while 43% had the same visual acuity in both of their eyes. 86% of the subjects were right-handed while 4% were left handed and 10% were ambidextrous. Significant correlations were found between visual acuity and eye preference and between visual acuity and eye dominance. The study also found a positive correlation between handedness and eye preference. These results support the notion that there is a weak correlation between the different aspects of lateralized behaviour in humans. 

  • 311.
    Chaabane, Wiem
    et al.
    Linköpings universitet, Medicinska fakulteten. Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för läkemedelsforskning.
    Lindqvist Appell, Malin
    Linköpings universitet, Institutionen för medicin och hälsa, Avdelningen för läkemedelsforskning. Linköpings universitet, Medicinska fakulteten.
    Interconnections between apoptotic and autophagic pathways during thiopurine-induced toxicity in cancer cells: the role of reactive oxygen species2016Ingår i: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 7, nr 46, s. 75616-75634Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Thiopurines (azathioprine, 6-mercaptopurine and 6-thioguanine) are a class of genotoxic drugs extensively used in the treatment of various illnesses including leukemia. Their underlying molecular mechanism of action involves the activation of apoptosis and autophagy but remains widely unclear. Here we present evidence that autophagy induction by thiopurines is a survival mechanism that antagonizes apoptosis and is involved in degrading damaged mitochondria through mitophagy. On the other hand, apoptosis is the main cell death mechanism by thiopurines as its inhibition prohibited cell death. Thus a tight interplay between apoptosis and autophagy controls cell fate in response to thiopurine treatment. Moreover, thiopurines disrupt mitochondrial function and induce a loss of the mitochondrial transmembrane potential. The involvement of the mitochondrial pathway in thiopurine-induced apoptosis was further confirmed by increased formation of reactive oxygen species (ROS). Inhibiting oxidative stress protected the cells from thiopurine-induced cell death and ROS scavenging prohibited autophagy induction by thiopurines. Our data indicate that the anticarcinogenic effects of thiopurines are mediated by complex interplay between cellular mechanisms governing redox homeostasis, apoptosis and autophagy.

  • 312.
    Chasapis, Christos T.
    et al.
    Hellas Forth, Greece.
    Makridakis, Manousos
    Acad Athens BRFAA, Greece.
    Damdimopoulos, Anastassios E.
    Karolinska Inst, Sweden.
    Zoidakis, Jerome
    Acad Athens BRFAA, Greece.
    Lygirou, Vasiliki
    Acad Athens BRFAA, Greece.
    Mavroidis, Manolis
    Acad Athens BRFAA, Greece.
    Vlahou, Antonia
    Acad Athens BRFAA, Greece.
    Miranda-Vizuete, Antonio
    Univ Seville, Spain.
    Spyrou, Giannis
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för klinisk kemi. Linköpings universitet, Medicinska fakulteten.
    Vlamis-Gardikas, Alexios
    Univ Patras, Greece.
    Implications of the mitochondrial interactome of mammalian thioredoxin 2 for normal cellular function and disease2019Ingår i: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 137, s. 59-73Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Multiple thioredoxin isoforms exist in all living cells. To explore the possible functions of mammalian mitochondrial thioredoxin 2 (Trx2), an interactome of mouse Trx2 was initially created using (i) a monothiol mouse Trx2 species for capturing protein partners from different organs and (ii) yeast two hybrid screens on human liver and rat brain cDNA libraries. The resulting interactome consisted of 195 proteins (Trx2 included) plus the mitochondrial 16S RNA. 48 of these proteins were classified as mitochondrial (MitoCarta2.0 human inventory). In a second step, the mouse interactome was combined with the current four-membered mitochondrial sub-network of human Trx2 (BioGRID) to give a 53-membered human Trx2 mitochondrial interactome (52 interactor proteins plus the mitochondrial 16S RNA). Although thioredoxins are thiol-employing disulfide oxidoreductases, approximately half of the detected interactions were not due to covalent disulfide bonds. This finding reinstates the extended role of thioredoxins as moderators of protein function by specific non-covalent, protein-protein interactions. Analysis of the mitochondrial interactome suggested that human Trx2 was involved potentially in mitochondrial integrity, formation of iron sulfur clusters, detoxification of aldehydes, mitoribosome assembly and protein synthesis, protein folding, ADP ribosylation, amino acid and lipid metabolism, glycolysis, the TCA cycle and the electron transport chain. The oxidoreductase functions of Trx2 were verified by its detected interactions with mitochondrial peroxiredoxins and methionine sulfoxide reductase. Parkinsons disease, triosephosphate isomerase deficiency, combined oxidative phosphorylation deficiency, and lactate dehydrogenase b deficiency are some of the diseases where the proposed mitochondrial network of Trx2 may be implicated.

  • 313.
    Chaskiel, Lea
    et al.
    Univ Bordeaux, France.
    Bristow, Adrian D.
    Natl Inst Biol Stand and Controls, England.
    Bluthe, Rose-Marie
    Univ Bordeaux, France.
    Dantzer, Robert
    Univ Texas Houston, TX 77030 USA.
    Blomqvist, Anders
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelning för neurobiologi. Linköpings universitet, Medicinska fakulteten.
    Konsman, Jan Pieter
    Univ Bordeaux, France.
    Interleukin-1 reduces food intake and body weight in rat by acting in the arcuate hypothalamus2019Ingår i: Brain, behavior, and immunity, ISSN 0889-1591, E-ISSN 1090-2139, Vol. 81, s. 560-573Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A reduction in food intake is commonly observed after bacterial infection, a phenomenon that can be reproduced by peripheral administration of Gram-negative bacterial lipopolysaccharide (LPS) or interleukin-lbeta (IL-1 beta), a pro-inflammatory cytokine released by LPS-activated macrophages. The arcuate nucleus of the hypothalamus (ARH) plays a major role in food intake regulation and expresses IL-1 type 1 receptor (IL-1R1) mRNA. In the present work, we tested the hypothesis that IL-1R1 expressing cells in the ARH mediate IL-1 beta and/or LPS-induced hypophagia in the rat. To do so, we developed an IL-1 beta-saporin conjugate, which eliminated IL-R1 expressing neurons in the hippocampus, and micro-injected it into the ARH prior to systemic IL-1 beta and LPS administration. ARH IL-1 beta-saporin injection resulted in loss of neuropeptide Y-containing cells and attenuated hypophagia and weight loss after intraperitoneal IL-1 beta, but not LPS, administration. In conclusion, the present study shows that ARH NPY-containing neurons express functional IL-1R1s that mediate peripheral IL-1 beta-, but not LPS-, induced hypophagia. Our present and previous findings indicate that the reduction of food intake after IL-1 beta and LPS are mediated by different neural pathways.

    Publikationen är tillgänglig i fulltext från 2020-07-13 13:53
  • 314.
    Chen, Gefei
    et al.
    Karolinska Institute, Sweden.
    Abelein, Axel
    Karolinska Institute, Sweden.
    Nilsson, Harriet E.
    Karolinska Institute, Sweden; KTH Royal Institute Technology, Sweden.
    Leppert, Axel
    Karolinska Institute, Sweden.
    Andrade-Talavera, Yuniesky
    Karolinska Institute, Sweden.
    Tambaro, Simone
    Karolinska Institute, Sweden.
    Hemmingsson, Lovisa
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten. Karolinska Institute, Sweden.
    Roshan, Firoz
    Karolinska Institute, Sweden.
    Landreh, Michael
    University of Oxford, England; Karolinska Institute, Sweden.
    Biverstal, Henrik
    Karolinska Institute, Sweden; Latvian Institute Organ Synth, Latvia.
    Koeck, Philip J. B.
    Karolinska Institute, Sweden; KTH Royal Institute Technology, Sweden.
    Presto, Jenny
    Karolinska Institute, Sweden.
    Hebert, Hans
    Karolinska Institute, Sweden; KTH Royal Institute Technology, Sweden.
    Fisahn, Andre
    Karolinska Institute, Sweden.
    Johansson, Jan
    Karolinska Institute, Sweden.
    Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state2017Ingår i: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 8, artikel-id 2081Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    . Protein misfolding and aggregation is increasingly being recognized as a cause of disease. In Alzheimers disease the amyloid-beta peptide (A beta) misfolds into neurotoxic oligomers and assembles into amyloid fibrils. The Bri2 protein associated with Familial British and Danish dementias contains a BRICHOS domain, which reduces A beta fibrillization as well as neurotoxicity in vitro and in a Drosophila model, but also rescues proteins from irreversible nonfibrillar aggregation. How these different activities are mediated is not known. Here we show that Bri2 BRICHOS monomers potently prevent neuronal network toxicity of A beta, while dimers strongly suppress A beta fibril formation. The dimers assemble into high-molecular-weight oligomers with an apparent two-fold symmetry, which are efficient inhibitors of non-fibrillar protein aggregation. These results indicate that Bri2 BRICHOS affects qualitatively different aspects of protein misfolding and toxicity via different quaternary structures, suggesting a means to generate molecular chaperone diversity.

  • 315.
    Cheung Mak, Wing
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Kwan Yee, Cheung
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Orban, Jenny
    Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik.
    Lee, Chyan-Jang
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Sensorvetenskap och Molekylfysik. Linköpings universitet, Tekniska högskolan.
    Turner, Anthony
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biosensorer och bioelektronik. Linköpings universitet, Tekniska fakulteten.
    Griffith, May
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Surface-Engineered Contact Lens as an Advanced Theranostic Platform for Modulation and Detection of Viral Infection2015Ingår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, nr 45, s. 25487-25494Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    We have demonstrated an entirely new concept of a wearable theranostic device in the form of a contact lens (theranostic lens) with a dual-functional hybrid surface to modulate and detect a pathogenic attack, using a the corneal HSV serotype-1 (HSV-1) model. The theranostic lenses were constructed using a facile layer-by-layer surface engineering technique, keeping the theranostic lenses with good surface wettability, optically transparency, and nontoxic toward human corneal epithelial cells. The theranostic lenses were used to capture and concentrate inflammatory cytokines such as interleukin-1 alpha (IL-1 alpha), which is upregulated during HSV-1 reactivation, for sensitive, noninvasive diagnostics. The theranostic lens also incorporated an antiviral coating to serve as a first line of defense to protect patients against disease. Our strategy tackles major problems in tear diagnostics that are mainly associated with the sampling of a relatively small volume of fluid and the low concentration of biomarkers. The theranostic lenses show effective anti-HSV-1 activity and good analytical performance for the detection of IL-1a, with a limit of detection of 1.43 pg mL(-1) and a wide linear range covering the clinically relevant region. This work offers a new paradigm for wearable noninvasive healthcare devices combining diagnosis and protection against disease, while supporting patient compliance. We believe that this approach holds immense promise as a next-generation point-of-care and decentralized diagnostic/theranostic platform for a range of biomarkers.

  • 316. Chlichlia, K.
    et al.
    Los, Marek Jan
    Department of Immunology and Cell Biology, University of Muenster, Roentgenstr. 21, D-48149 Muenster, Germany.
    Schulze-Osthoff, Klaus
    Department of Immunology and Cell Biology, University of Muenster, Roentgenstr. 21, D-48149 Muenster, Germany.
    Gazzolo, L.
    INSERM U412, Ecole Normale S périeure de Lyon, 69367 Lyon, Cedex 07, France.
    Schirrmacher, V.
    Division of Cellular Immunology (G0100), Tumor Immunology Program, German Cancer Research Center, Im Neuenheimer Feld 280, D69120 Heidelberg, Germany.
    Khazaie, K.
    Division of Cellular Immunology (G0100), Tumor Immunology Program, German Cancer Research Center, Im Neuenheimer Feld 280, D69120 Heidelberg, Germany; 4Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, U.S.A..
    Redox events in HTLV-1 tax-induced apoptotic T-cell death2002Ingår i: Antioxidants and Redox Signaling, ISSN 1523-0864, E-ISSN 1557-7716, Vol. 4, nr 3, s. 471-477Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A number of studies implicate reactive oxygen intermediates in the induction of DNA damage and apoptosis. Recent studies suggest that the human T-cell leukemia virus type I (HTLV-1) Tax protein induces oxidative stress and apoptotic T-cell death. Activation of the T-cell receptor/CD3 pathway enhances the Tax-mediated oxidative and apoptotic effects. Tax-mediated apoptosis and oxidative stress as well as activation of nuclear factor-kappaB can be potently suppressed by antioxidants. This review focuses on Tax-dependent changes in the intracellular redox status and their role in Tax-mediated DNA damage and apoptosis. The relevance of these observations to HTLV-1 virus-mediated T-cell transformation and leukemogenesis are discussed.

  • 317.
    Choong, Ferdinand X.
    et al.
    Karolinska Inst, Sweden.
    Bäck, Marcus
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
    Schulz, Anette
    Karolinska Inst, Sweden.
    Nilsson, Peter
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
    Edlund, Ulrica
    KTH Royal Inst Technol, Sweden.
    Richter-Dahlfors, Agneta
    Karolinska Inst, Sweden.
    Stereochemical identification of glucans by oligothiophenes enables cellulose anatomical mapping in plant tissues2018Ingår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, artikel-id 3108Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Efficient use of plant-derived materials requires enabling technologies for non-disruptive composition analysis. The ability to identify and spatially locate polysaccharides in native plant tissues is difficult but essential. Here, we develop an optical method for cellulose identification using the structure-responsive, heptameric oligothiophene h-FTAA as molecular fluorophore. Spectrophotometric analysis of h-FTAA interacting with closely related glucans revealed an exceptional specificity for beta-linked glucans. This optical, non-disruptive method for stereochemical differentiation of glycosidic linkages was next used for in situ composition analysis in plants. Multi-laser/multi-detector analysis developed herein revealed spatial localization of cellulose and structural cell wall features such as plasmodesmata and perforated sieve plates of the phloem. Simultaneous imaging of intrinsically fluorescent components revealed the spatial relationship between cell walls and other organelles, such as chloroplasts and lignified annular thickenings of the trachea, with precision at the sub-cellular scale. Our non-destructive method for cellulose identification lays the foundation for the emergence of anatomical maps of the chemical constituents in plant tissues. This rapid and versatile method will likely benefit the plant science research fields and may serve the biorefinery industry as reporter for feedstock optimization as well as in-line monitoring of cellulose reactions during standard operations.

  • 318.
    Choudhury, Md. Maidul Islam
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Ekologi. Linköpings universitet, Tekniska högskolan.
    Does an evolutionary change in the water sowbug Asellus aquaticus L. alter its functional role?2011Självständigt arbete på avancerad nivå (masterexamen), 60 poäng / 90 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The ecology behind evolutionary diversification is a well studied area of research, whereas the effects of evolution on ecosystems get little attention. In line with ecological theory, evolutionary diversification of a species could influence different ecosystem aspects such as food web composition, energy flow, nutrient cycling etc. The main objective of this study was to investigate whether two diverging ecotypes (reed and chara) of Asellus aquaticus differ regarding their role in two aquatic ecosystem processes: decomposition of terrestrial leaves and grazing of periphyton. Their role in ecosystem process as well as treatment effects on fitness, measured as growth and survival, were investigated in a laboratory experiment with various levels of intra-specific competition and inter-specific interactions with the amphipod Gammarus pulex. The isopods were collected from two Swedish lakes: Lake Tåkern and Lake Fardume. These two lakes represent different history of ecotype divergence. The experimental design consisted of 2-L aquaria, each providing elm leaves (Ulmus glabra), oak leaves (Quercus roburleaves) and periphyton as food sources. Ten treatments with five replicates were applied for each lake and the experiment lasted for four weeks. The study showed that there was no significant difference between chara and reed ecotype in their functional role. However, the rate of ecosystem processes per individual decreased in competitive interactions. In high density, decomposition per dry weight consumer was low and total algae biomass was high at the end of four weeks due to intra-specific competition. Moreover, ecosystem processes were lowest in inter-specific competition between Gammarus pulex and each ecotype. Present study also shows that ecotypes from the different lakes, having different history, had different responses to mortality and growth. 

  • 319.
    Christianou, Maria
    Linköpings universitet, Institutionen för fysik och mätteknik. Linköpings universitet, Tekniska högskolan.
    Interaction strength and keystone species in model food webs2003Licentiatavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Ecological communities are often exposed to different kind of disturbances of varied magnitude. The response to disturbances can be affected by, among other factors, the strength of interactions between species within the community. The main purpose of this thesis is the study of the relation between interaction strength (distribution and positioning of strong and weak links) and the response of model communities to disturbances. Additionally, we wish to identify keystone species and keystone links and to recognize species vulnerable to secondary extinctions with respect to the interaction strength concept.

    The first part of this study (paper I) deals with species removal from model ecological communities. The number of secondary extinctions following the loss of one species measures the response to this disturbance. The loss of the following species categories triggered, on average, the largest number of secondary extinctions: a) rare resources strongly interacting with many consumers b) abundant intermediate consumer species strongly interacting with many resources and c) abundant weakly interacting resources. Species vulnerable to secondary extinctions were mainly weakly interacting consumers and strongly interacting resources (excluding primary producers). This vulnerability to secondary extinctions was not only dependent on characteristics of the species themselves but also on which species was initially removed. Some species were facing a high probability of extinction after the deletion of a wide range of species categories, while others were vulnerable only to the deletion of few species categories.

    The second part (paper II) deals with less severe disturbances; small changes in the intrinsic growth rate of species and small changes in the interaction strength of links. In this way the importance of both species and links was revealed. The response of the community was measured as the effect of these changes on the resilience of the system. Overall, the resilience of the model communities was more sensitive to changes in weakly interacting species or weak links.

    The main message of this work is that keystone species and keystone links could be context dependent, both with respect to characteristics of the community and the stability criterion used. Studying the effects of severe disturbances, such as species loss, and using the number of secondary extinctions to measure the community's response, the disturbance of strongly interacting species affected the community most. On the contrary, by applying small perturbations to community parameters and measuring the response as the effect on resilience, the perturbation of weakly interacting species and weak links affected the community most. Thus, both strong and weak agents of the community can be important for stability properties under different regimes of disturbance.

  • 320.
    Christoffersson, Jonas
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
    Aronsson, Christopher
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Jury, Michael
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Selegård, Robert
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Aili, Daniel
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär fysik. Linköpings universitet, Tekniska fakulteten.
    Mandenius, Carl-Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska fakulteten.
    Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device2018Ingår i: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 11, nr 1, s. 1-13, artikel-id 015013Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Liver cell culture models are attractive in both tissue engineering and for development of assays for drug toxicology research. To retain liver specific cell functions, the use of adequate cell types and culture conditions, such as a 3D orientation of the cells and a proper supply of nutrients and oxygen, are critical. In this article, we show how extracellular matrix mimetic hydrogels can support hepatocyte viability and functionality in a perfused liver-on-a-chip device. A modular hydrogel system based on hyaluronan and poly(ethylene glycol) (HA-PEG), modified with cyclooctyne moieties for bioorthogonal strain-promoted alkyne-azide 1, 3-dipolar cycloaddition (SPAAC), was developed, characterized, and compared for cell compatibility to hydrogels based on agarose and alginate. Hepatoma cells (HepG2) formed spheroids with viable cells in all hydrogels with the highest expression of albumin and urea in alginate hydrogels. By including an excess of cyclooctyne in the HA backbone, azide-modified cell adhesion motifs (linear and cyclic RGD peptides) could be introduced in order to enhance viability and functionality of human induced pluripotent stem cell derived hepatocytes (hiPS-HEPs). In the HA-PEG hydrogels modified with cyclic RGD peptides hiPS-HEPs migrated and grew in 3D and showed an increased viability and higher albumin production compared to when cultured in the other hydrogels. This flexible SPAAC crosslinked hydrogel system enabled fabrication of perfused 3D cell culture of hiPS-HEPs and is a promising material for further development and optimization of liver-on-a-chip devices.

  • 321.
    Cirtwill, Alyssa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten.
    Dalla Riva, Giulio V.
    Department of Statistics, University of British Columbia, Vancouver, Canada; School of Mathematics and Statistics, University of Canterbury, Christchurch, New Zealand.
    Gaiarsa, Marilia P.
    Departamento de Ecologia Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil; Department of Entomology, University of California, Riverside, California, USA.
    Bimler, Malyon D.
    School of Biological Sciences, University of Queensland, Brisbane, Australia.
    Cagua, E. Fernando
    Centre for Integrative Ecology, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
    Coux, Camille
    UMR 1065 Santé et Agroécologie du Vignoble, INRA Bordeaux-Aquitaine, France; Centre d’Etudes Biologiques de Chizé UMR 7372, CNRS, Université de La Rochelle, France.
    Dehling, D. Matthias
    Centre for Integrative Ecology, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
    A review of species role concepts in food webs2018Ingår i: Food Webs, E-ISSN 2352-2496, Vol. 16, artikel-id e00093Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Many different concepts have been used to describe species' roles in food webs (i.e., the ways in which species participate in their communities as consumers and resources). As each concept focuses on a different aspect of food-web structure, it can be difficult to relate these concepts to each other and to other aspects of ecology. Here we use the Eltonian niche as an overarching framework, within which we summarize several commonly-used role concepts (degree, trophic level, motif roles, and centrality). We focus mainly on the topological versions of these concepts but, where dynamical versions of a role concept exist, we acknowledge these as well. Our aim is to highlight areas of overlap and ambiguity between different role concepts and to describe how these roles can be used to group species according to different strategies (i.e., equivalence and functional roles). The existence of “gray areas” between role concepts make it essential for authors to carefully consider both which role concept(s) are most appropriate for the analyses they wish to conduct and what aspect of species' niches (if any) they wish to address. The ecological meaning of differences between species' roles can change dramatically depending on which role concept(s) are used.

  • 322.
    Cirtwill, Alyssa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten. Stockholm Univ, Sweden.
    Eklöf, Anna
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten.
    Roslin, Tomas
    Swedish Univ Agr Sci, Sweden.
    Wootton, Kate
    Swedish Univ Agr Sci, Sweden.
    Gravel, Dominique
    Univ Sherbrooke, Canada.
    A quantitative framework for investigating the reliability of empirical network construction2019Ingår i: Methods in Ecology and Evolution, ISSN 2041-210X, E-ISSN 2041-210X, Vol. 10, nr 6, s. 902-911Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Descriptions of ecological networks typically assume that the same interspecific interactions occur each time a community is observed. This contrasts with the known stochasticity of ecological communities: community composition, species abundances and link structure all vary in space and time. Moreover, finite sampling generates variation in the set of interactions actually observed. For interactions that have not been observed, most datasets will not contain enough information for the ecologist to be confident that unobserved interactions truly did not occur. Here, we develop the conceptual and analytical tools needed to capture uncertainty in the estimation of pairwise interactions. To define the problem, we identify the different contributions to the uncertainty of an interaction. We then outline a framework to quantify the uncertainty around each interaction by combining data on observed co-occurrences with prior knowledge. We illustrate this framework using perhaps the most extensively sampled network to date. We found significant uncertainty in estimates for the probability of most pairwise interactions. This uncertainty can, however, be constrained with informative priors. This uncertainty scaled up to summary measures of network structure such as connectance and nestedness. Even with informative priors, we are likely to miss many interactions that may occur rarely or under different local conditions. Overall, we demonstrate the importance of acknowledging the uncertainty inherent in network studies, and the utility of treating interactions as probabilities in pinpointing areas where more study is needed. Most importantly, we stress that networks are best thought of as systems constructed from random variables, the stochastic nature of which must be acknowledged for an accurate representation. Doing so will fundamentally change network analyses and yield greater realism.

  • 323.
    Cirtwill, Alyssa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten. University of Canterbury, New Zealand; University of Otago, New Zealand.
    Lagrue, Clement
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Poulin, Robert
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Stouffer, Daniel B.
    University of Canterbury, New Zealand.
    Host taxonomy constrains the properties of trophic transmission routes for parasites in lake food webs2017Ingår i: Ecology, ISSN 0012-9658, E-ISSN 1939-9170, Vol. 98, nr 9, s. 2401-2412Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Some parasites move from one host to another via trophic transmission, the consumption of the parasite (inside its current host) by its future host. Feeding links among free-living species can thus be understood as potential transmission routes for parasites. As these links have different dynamic and structural properties, they may also vary in their effectiveness as trophic transmission routes. That is, some links may be better than others in allowing parasites to complete their complex life cycles. However, not all links are accessible to parasites as most are restricted to a small number of host taxa. This restriction means that differences between links involving host and non-host taxa must be considered when assessing whether transmission routes for parasites have different food web properties than other links. Here we use four New Zealand lake food webs to test whether link properties (contribution of a link to the predators diet, prey abundance, prey biomass, amount of biomass transferred, centrality, and asymmetry) affect trophic transmission of parasites. Critically, we do this using both models that neglect the taxonomy of free-living species and models that explicitly include information about which free-living species are members of suitable host taxa. Although the best-fit model excluding taxonomic information suggested that transmission routes have different properties than other feeding links, when including taxonomy, the best-fit model included only an intercept. This means that the taxonomy of free-living species is a key determinant of parasite transmission routes and that food-web properties of transmission routes are constrained by the properties of host taxa. In particular, many intermediate hosts (prey) attain high biomasses and are involved in highly central links while links connecting intermediate to definitive (predator) hosts tend to be dynamically weak.

  • 324.
    Cirtwill, Alyssa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten. Univ Canterbury, New Zealand.
    Roslin, Tomas
    Swedish Univ Agr Sci, Sweden; Univ Helsinki, Finland.
    Rasmussen, Claus
    Aarhus Univ, Denmark.
    Olesen, Jens Mogens
    Aarhus Univ, Denmark.
    Stouffer, Daniel B.
    Univ Canterbury, New Zealand.
    Between-year changes in community composition shape species' roles in an Arctic plant-pollinator network2018Ingår i: Oikos, ISSN 0030-1299, E-ISSN 1600-0706, Vol. 127, nr 8, s. 1163-1176Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Inter-annual turnover in community composition can affect the richness and functioning of ecological communities. If incoming and outgoing species do not interact with the same partners, ecological functions such as pollination may be disrupted. Here, we explore the extent to which turnover affects species' roles - as defined based on their participation in different motifs positions - in a series of temporally replicated plant-pollinator networks from high-Arctic Zackenberg, Greenland. We observed substantial turnover in the plant and pollinator assemblages, combined with significant variation in species' roles between networks. Variation in the roles of plants and pollinators tended to increase with the amount of community turnover, although a negative interaction between turnover in the plant and pollinator assemblages complicated this trend for the roles of pollinators. This suggests that increasing turnover in the future will result in changes to the roles of plants and likely those of pollinators. These changing roles may in turn affect the functioning or stability of this pollination network.

  • 325.
    Cirtwill, Alyssa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten.
    Eklöf, Anna
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska fakulteten.
    Feeding environment and other traits shape species roles in marine food webs2018Ingår i: Ecology Letters, ISSN 1461-023X, E-ISSN 1461-0248, Vol. 21, nr 6, s. 875-884Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Food webs and meso-scale motifs allow us to understand the structure of ecological communities and define species roles within them. This species-level perspective on networks permits tests for relationships between species traits and their patterns of direct and indirect interactions. Such relationships could allow us to predict food-web structure based on more easily obtained trait information. Here, we calculated the roles of species (as vectors of motif position frequencies) in six well-resolved marine food webs and identified the motif positions associated with the greatest variation in species roles. We then tested whether the frequencies of these positions varied with species traits. Despite the coarse-grained traits we used, our approach identified several strong associations between traits and motifs. Feeding environment was a key trait in our models and may shape species roles by affecting encounter probabilities. Incorporating environment into future food-web models may improve predictions of an unknown network structure.

  • 326.
    Clayton, Zoe E.
    et al.
    Heart Res Inst, Australia.
    Tan, Richard P.
    Heart Res Inst, Australia; Univ Sydney, Australia.
    Miravet, Maria M.
    Linköpings universitet, Medicinska fakulteten. Heart Res Inst, Australia.
    Lennartsson, Katarina
    Linköpings universitet, Medicinska fakulteten. Heart Res Inst, Australia.
    Cooke, John P.
    Houston Methodist Res Inst, TX 77030 USA.
    Bursill, Christina A.
    Heart Res Inst, Australia.
    Wise, Steven G.
    Heart Res Inst, Australia.
    Patel, Sanjay
    Heart Res Inst, Australia; Univ Sydney, Australia.
    Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing mode2018Ingår i: Bioscience Reports, ISSN 0144-8463, E-ISSN 1573-4935, Vol. 38, artikel-id BSR20180563Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Chronic wounds are a major complication in patients with cardiovascular diseases. Cell therapies have shown potential to stimulate wound healing, but clinical trials using adult stem cells have been tempered by limited numbers of cells and invasive procurement procedures. Induced pluripotent stem cells (iPSCs) have several advantages of other cell types, for example they can be generated in abundance from patients somatic cells (autologous) or those from a matched donor. iPSCs can be efficiently differentiated to functional endothelial cells (iPSC-ECs). Here, we used a murine excisional wound model to test the pro-angiogenic properties of iPSC-ECs in wound healing. Two full-thickness wounds were made on the dorsum of NOD-SCID mice and splinted. iPSC-ECs (5 x 10(5)) were topically applied to one wound, with the other serving as a control. Treatment with iPSC-ECs significantly increased wound perfusion and accelerated wound closure. Expression of endothelial cell (EC) surface marker, platelet endothelial cell adhesion molecule (PECAM-1) (CD31), and pro-angiogenic EC receptor, Tie1, mRNA was up-regulated in iPSC-EC treated wounds at 7 days post-wounding. Histological analysis of wound sections showed increased capillary density in iPSC-EC wounds at days 7 and 14 post-wounding, and increased collagen content at day 14. Anti-GFP fluorescence confirmed presence of iPSC-ECs in the wounds. Bioluminescent imaging (BLI) showed progressive decline of iPSC-ECs over time, suggesting that iPSC-ECs are acting primarily through short-term paracrine effects. These results highlight the pro-regenerative effects of iPSC-ECs and demonstrate that they are a promising potential therapy for intractable wounds.

  • 327.
    Collins, Matthew D.
    et al.
    School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
    Routh, Joyanto
    Department of Geology and Geochemistry, Stockholm University, Stockholm, Sweden .
    Saraswathy, Ambujom
    Department of Geology and Geochemistry, Stockholm University, Stockholm, Sweden .
    Lawson, Paul A.
    School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK.
    Schumann, Peter
    DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
    Welinder-Olsson, Christina
    Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
    Falsen, Enevold
    Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden.
    Arsenicicoccus bolidensis gen. nov., sp. nov., a novel actinomycete isolated from contaminated lake sediment2004Ingår i: International Journal of Systematic and Evolutionary Microbiology, ISSN 1466-5026, E-ISSN 1466-5034, Vol. 54, nr 2, s. 605-608Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    An unknown Gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium originating from sediment was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on ll-diaminopimelic acid (type ll-Dpm-glycine1), a complex mixture of saturated, monounsaturated and iso- and anteiso-methyl-branched, non-hydroxylated, long-chain cellular fatty acids and tetrahydrogenated menaquinones with eight isoprene units [MK-8(H4)] as the major respiratory lipoquinone. This combination of characteristics somewhat resembled members of the suborder Micrococcineae, but did not correspond to any currently described species. Comparative 16S rRNA gene sequencing confirmed that the unidentified coccus-shaped organism is a member of the Actinobacteria and represents a hitherto-unknown subline related to, albeit different from, a number of taxa including Intrasporangium, Janibacter, Terrabacter, Terracoccus and Ornithinicoccus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium originating from lake sediment be classified as a new genus and species, Arsenicicoccus bolidensis gen. nov., sp. nov. (type strain CCUG 47306T=DSM 15745T).

  • 328.
    Conti, Luca
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Renhorn, Jakob
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Gabrielsson, Anders
    KTH Royal Institute Technology, Sweden.
    Turesson, Fredrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Medicinska fakulteten.
    Liin, Sara
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Lindahl, Erik
    KTH Royal Institute Technology, Sweden; Stockholm University, Sweden.
    Elinder, Fredrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Medicinska fakulteten.
    Reciprocal voltage sensor-to-pore coupling leads to potassium channel C-type inactivation2016Ingår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, artikel-id 27562Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Voltage-gated potassium channels open at depolarized membrane voltages. A prolonged depolarization causes a rearrangement of the selectivity filter which terminates the conduction of ions - a process called slow or C-type inactivation. How structural rearrangements in the voltage-sensor domain (VSD) cause alteration in the selectivity filter, and vice versa, are not fully understood. We show that pulling the pore domain of the Shaker potassium channel towards the VSD by a Cd2+ bridge accelerates C-type inactivation. Molecular dynamics simulations show that such pulling widens the selectivity filter and disrupts the K+ coordination, a hallmark for C-type inactivation. An engineered Cd2+ bridge within the VSD also affect C-type inactivation. Conversely, a pore domain mutation affects VSD gating-charge movement. Finally, C-type inactivation is caused by the concerted action of distant amino acid residues in the pore domain. All together, these data suggest a reciprocal communication between the pore domain and the VSD in the extracellular portion of the channel.

  • 329.
    Coorens, Maarten
    et al.
    Karolinska Inst, Sweden.
    Rao, Anna
    Karolinska Inst, Sweden.
    Grafe, Stefanie Katharina
    Karolinska Inst, Sweden.
    Unelius, Daniel
    Karolinska Inst, Sweden.
    Lindforss, Ulrik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Medicinska fakulteten.
    Agerberth, Birgitta
    Karolinska Inst, Sweden.
    Mjosberg, Jenny
    Karolinska Inst, Sweden.
    Bergman, Peter
    Karolinska Inst, Sweden.
    Innate lymphoid cell type 3-derived interleukin-22 boosts lipocalin-2 production in intestinal epithelial cells via synergy between STAT3 and NF-B2019Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 294, nr 15, s. 6027-6041Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Escherichia coli and Klebsiella pneumoniae are opportunistic pathogens that are commonly associated with infections at mucosal surfaces, such as the lung or the gut. The host response against these types of infections includes the release of epithelial-derived antimicrobial factors such as lipocalin-2 (LCN-2), a protein that specifically inhibits the iron acquisition of Enterobacteriaceae by binding and neutralizing the bacterial iron-scavenging molecule enterobactin. Regulation of epithelial antimicrobial responses, including the release of LCN-2, has previously been shown to depend on IL-22, a cytokine produced by innate lymphoid cells type 3 (ILC3) during Enterobacteriaceae infections. However, much remains unknown about the extent to which antimicrobial responses are regulated by IL-22 and how IL-22 regulates the expression and production of LCN-2 in intestinal epithelial cells (IECs). Our study demonstrates how IL-22-induced activation of STAT3 synergizes with NF-B-activating cytokines to enhance LCN-2 expression in human IECs and elucidates how ILC3 are involved in LCN-2-mediated host defense against Enterobacteriaceae. Together, these results provide new insight into the role of ILC3 in regulating LCN-2 expression in human IECs and could prove useful in future studies aimed at understanding the host response against Enterobacteriaceae as well as for the development of antimicrobial therapies against Enterobacteriaceae-related infections.

  • 330.
    Cossarizza, Andrea
    et al.
    Univ Modena and Reggio Emilia, Italy.
    Chang, Hyun-Dong
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Radbruch, Andreas
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Acs, Andreas
    Friedrich Alexander Univ Erlangen Nuremberg, Germany.
    Adam, Dieter
    Christian Albrechts Univ Kiel, Germany.
    Adam-Klages, Sabine
    Univ Klin Schleswig Holstein, Germany.
    Agace, William W.
    Tech Univ Denmark, Denmark; Lund Univ, Sweden.
    Aghaeepour, Nima
    Stanford Univ, CA 94305 USA; Stanford Univ, CA 94305 USA; Stanford Univ, CA 94305 USA.
    Akdis, Muebeccel
    Univ Zurich, Switzerland.
    Allez, Matthieu
    Univ Paris, France; Hop St Louis, France.
    Almeida, Larissa Nogueira
    Univ Lubeck, Germany.
    Alvisi, Giorgia
    Humanitas Clin and Res Ctr, Italy.
    Anderson, Graham
    Univ Birmingham, England.
    Andrae, Immanuel
    Tech Univ Munich, Germany.
    Annunziato, Francesco
    Univ Florence, Italy.
    Anselmo, Achille
    Humanitas Clin and Res Ctr, Italy.
    Bacher, Petra
    Christian Albrechts Univ Kiel, Germany; Christian Albrechts Univ Kiel, Germany.
    Baldari, Cosima T.
    Univ Siena, Italy.
    Bari, Sudipto
    Natl Canc Ctr Singapore, Singapore; Duke NUS Med Sch, Singapore.
    Barnaba, Vincenzo
    Sapienza Univ Roma, Italy; Ist Italiano Tecnol, Italy; Fdn Cenci Bolognetti, Italy.
    Barros-Martins, Joana
    Hannover Med Sch, Germany.
    Battistini, Luca
    IRCCS Fdn Santa Lucia, Italy.
    Bauer, Wolfgang
    Med Univ Vienna, Austria.
    Baumgart, Sabine
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Baumgarth, Nicole
    Univ Calif Davis, CA 95616 USA; Univ Calif Davis, CA 95616 USA.
    Baumjohann, Dirk
    Ludwig Maximilians Univ Munchen, Germany.
    Baying, Bianka
    EMBL, Germany.
    Bebawy, Mary
    Univ Technol Sydney, Australia.
    Becher, Burkhard
    Univ Zurich, Switzerland; Comprehens Canc Ctr Zurich, Switzerland.
    Beisker, Wolfgang
    German Res Ctr Environm Hlth, Germany.
    Benes, Vladimir
    EMBL, Germany.
    Beyaert, Rudi
    Ghent Univ VIB, Belgium.
    Blanco, Alfonso
    Univ Coll Dublin, Ireland.
    Boardman, Dominic A.
    Univ British Columbia, Canada; BC Childrens Hosp Res Inst, Canada.
    Bogdan, Christian
    Univ Klinikum Erlangen, Germany; Friedrich Alexander Univ FAU Erlangen Nurnberg, Germany; Med Immunol Campus Erlangen, Germany.
    Borger, Jessica G.
    Monash Univ, Australia.
    Borsellino, Giovanna
    Fdn Santa Lucia IRCCS, Italy; Fdn Santa Lucia IRCCS, Italy.
    Boulais, Philip E.
    Albert Einstein Coll Med, NY 10467 USA; Ruth L and David S Gottesman Inst Stem Cell and Regen, NY USA.
    Bradford, Jolene A.
    Thermo Fisher Sci, OR USA.
    Brenner, Dirk
    Luxembourg Inst Hlth, Luxembourg; Univ Southern Denmark, Denmark; Univ Southern Denmark, Denmark; Univ Luxembourg, Luxembourg.
    Brinkman, Ryan R.
    Univ British Columbia, Canada; BC Canc, Canada.
    Brooks, Anna E. S.
    Univ Auckland, New Zealand.
    Busch, Dirk H.
    Tech Univ Munich, Germany; German Ctr Infect Res DZIF, Germany; Tech Univ Munich, Germany.
    Buescher, Martin
    Miltenyi Biotec GmbH, Germany.
    Bushnell, Timothy P.
    Univ Rochester, NY 14642 USA; Univ Rochester, NY 14642 USA.
    Calzetti, Federica
    Univ Verona, Italy.
    Cameron, Garth
    Univ Melbourne, Australia.
    Cammarata, Ilenia
    Sapienza Univ Roma, Italy.
    Cao, Xuetao
    Nankai Univ, Peoples R China.
    Cardell, Susanna L.
    Univ Gothenburg, Sweden.
    Casola, Stefano
    FIRC Inst Mol Oncol FOM, Italy.
    Cassatella, Marco A.
    Univ Verona, Italy.
    Cavani, Andrea
    Natl Inst Hlth Migrat and Poverty INMP, Italy.
    Celada, Antonio
    Univ Barcelona, Spain.
    Chatenoud, Lucienne
    Univ Paris 05, France.
    Chattopadhyay, Pratip K.
    NYU Langone Med Ctr, NY USA.
    Chow, Sue
    Princess Margaret Hosp, Canada.
    Christakou, Eleni
    Kings Coll London, England; Guys and St Thomas Natl Hlth Serv Fdn Trust, England; Kings Coll London, England.
    Cicin-Sain, Luka
    Helmholtz Ctr Infect Res, Germany.
    Clerici, Mario
    IRCCS Fdn Don Carlo Gnocchi, Italy; Univ Milan, Italy; Univ Milano Bicocca, Italy.
    Colombo, Federico S.
    Humanitas Clin and Res Ctr, Italy.
    Cook, Laura
    BC Childrens Hosp Res Inst, Canada; Univ British Columbia, Canada.
    Cooke, Anne
    Univ Cambridge, England.
    Cooper, Andrea M.
    Univ Leicester, England.
    Corbett, Alexandra J.
    Univ Melbourne, Australia.
    Cosma, Antonio
    Luxembourg Inst Hlth, Luxembourg.
    Cosmi, Lorenzo
    Univ Florence, Italy.
    Coulie, Pierre G.
    Catholic Univ Louvain, Belgium.
    Cumano, Ana
    Inst Pasteur, France.
    Cvetkovic, Ljiljana
    Univ Erlangen Nurnberg, Germany.
    Dang, Van Duc
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Dang-Heine, Chantip
    Charite Univ Med Berlin, Germany.
    Davey, Martin S.
    Monash Univ, Australia; Monash Univ, Australia; Monash Univ, Australia.
    Davies, Derek
    Francis Crick Inst, England.
    De Biasi, Sara
    Univ Modena and Reggio Emilia, Italy.
    Del Zotto, Genny
    IRCCS Ist Giannina Gaslini, Italy.
    Dela Cruz, Gelo Victoriano
    Univ Copenhagen, Denmark.
    Delacher, Michael
    Regensburg Ctr Intervent Immunol RCI, Germany; Univ Regensburg, Germany.
    Della Bella, Silvia
    Univ Milan, Italy.
    Dellabona, Paolo
    Ist Sci San Raffaele, Italy.
    Deniz, Guennur
    Istanbul Univ, Turkey.
    Dessing, Mark
    Sony Europe Ltd, England.
    Di Santo, James P.
    Inst Pasteur, France; Inst Pasteur, France.
    Diefenbach, Andreas
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany; Berlin Inst Hlth BIH, Germany.
    Dieli, Francesco
    Univ Palermo, Italy.
    Dolf, Andreas
    Univ Bonn, Germany.
    Doerner, Thomas
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Dress, Regine J.
    ASTAR, Singapore.
    Dudziak, Diana
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Dustin, Michael
    Univ Oxford, England.
    Dutertre, Charles-Antoine
    Duke NUS Med Sch, Singapore; ASTAR, Singapore.
    Ebner, Friederike
    Free Univ Berlin, Germany.
    Eckle, Sidonia B. G.
    Univ Melbourne, Australia.
    Edinger, Matthias
    Regensburg Ctr Intervent Immunol RCI, Germany; Univ Hosp Regensburg, Germany.
    Eede, Pascale
    Charite Univ Med Berlin, Germany; Free Univ Berlin, Germany; Humboldt Univ, Germany; Berlin Inst Hlth, Germany.
    Ehrhardt, Goetz R. A.
    Univ Toronto, Canada.
    Eich, Marcus
    Heidelberg Inst Stem Cell Technol and Expt Med HI S, Germany.
    Engel, Pablo
    Univ Barcelona, Spain.
    Engelhardt, Britta
    Univ Bern, Switzerland.
    Erdei, Anna
    Univ L Eotvos, Hungary.
    Esser, Charlotte
    Leibniz Res Inst Environm Med, Germany.
    Everts, Bart
    Leiden Univ, Netherlands.
    Evrard, Maximilien
    ASTAR, Singapore.
    Falk, Christine S.
    Hannover Med Sch, Germany.
    Fehniger, Todd A.
    Washington Univ, MO USA.
    Felipo-Benavent, Mar
    Principe Felipe Res Ctr, Spain.
    Ferry, Helen
    Univ Oxford, England.
    Feuerer, Markus
    Regensburg Ctr Intervent Immunol RCI, Germany; Univ Regensburg, Germany.
    Filby, Andrew
    Newcastle Univ, England.
    Filkor, Kata
    MDQuest Ltd, Hungary.
    Fillatreau, Simon
    Univ Paris 05, France.
    Follo, Marie
    Univ Freiburg, Germany; Univ Klinikum Freiburg, Germany.
    Foerster, Irmgard
    Univ Bonn, Germany.
    Foster, John
    Owl Biomed Inc, CA USA.
    Foulds, Gemma A.
    Nottingham Trent Univ, England.
    Frehse, Britta
    Univ Lubeck, Germany.
    Frenette, Paul S.
    Albert Einstein Coll Med, NY 10467 USA; Ruth L and David S Gottesman Inst Stem Cell and Regen, NY USA; Albert Einstein Coll Med, NY 10467 USA.
    Frischbutter, Stefan
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany; Free Univ Berlin, Germany; Humboldt Univ, Germany; Berlin Inst Hlth, Germany.
    Fritzsche, Wolfgang
    Leibniz Inst Photon Technol IPHT, Germany.
    Galbraith, David W.
    Univ Arizona, AZ USA; Univ Arizona, AZ USA; Henan Univ, Peoples R China.
    Gangaev, Anastasia
    Netherlands Canc Inst, Netherlands.
    Garbi, Natalio
    Univ Bonn, Germany.
    Gaudilliere, Brice
    Stanford Univ, CA 94305 USA.
    Gazzinelli, Ricardo T.
    Fundacao Oswaldo Cruz Minas, Brazil; Univ Massachusetts, MA USA.
    Geginat, Jens
    Fdn Ist Nazl Genet Mol Ronmeo and Enric Invernizzi, Italy.
    Gerner, Wilhelm
    Univ Vet Med Vienna, Austria; Univ Vet Med Vienna, Austria.
    Gherardin, Nicholas A.
    Univ Melbourne, Australia.
    Ghoreschi, Kamran
    Charite Univ Med Berlin, Germany.
    Gibellini, Lara
    Univ Modena and Reggio Emilia, Italy.
    Ginhoux, Florent
    ASTAR, Singapore; SingHlth Duke NUS Acad Med Ctr, Singapore; Shanghai Jiao Tong Univ, Peoples R China.
    Goda, Keisuke
    Univ Calif Los Angeles, CA USA; Univ Tokyo, Japan; Wuhan Univ, Peoples R China.
    Godfrey, Dale I.
    Univ Melbourne, Australia.
    Goettlinger, Christoph
    Univ Cologne, Germany.
    Gonzalez-Navajas, Jose M.
    Alicante Inst Hlth and Biomed Res ISABIAL, Spain; Networked Biomed Res Ctr Hepat and Digest Dis CIBER, Spain.
    Goodyear, Carl S.
    Univ Glasgow, Scotland.
    Gori, Andrea
    Univ Milan, Italy.
    Grogan, Jane L.
    Genentech Inc, CA USA.
    Grummitt, Daryl
    Owl Biomed Inc, CA USA.
    Gruetzkau, Andreas
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Haftmann, Claudia
    Univ Zurich, Switzerland.
    Hahn, Jonas
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Hammad, Hamida
    Fac Med and Hlth Sci, Belgium.
    Haemmerling, Guenter
    German Canc Res Ctr, Germany.
    Hansmann, Leo
    Berlin Inst Hlth BIH, Germany; German Canc Consortium DKTK, Germany; Charite Univ Med Berlin, Germany.
    Hansson, Goran
    Karolinska Univ Hosp, Sweden; Karolinska Univ Hosp, Sweden.
    Harpur, Christopher M.
    Murdoch Childrens Res Inst, Australia.
    Hartmann, Susanne
    Free Univ Berlin, Germany.
    Hauser, Andrea
    Univ Hosp Regensburg, Germany.
    Hauser, Anja E.
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany; Free Univ Berlin, Germany; Humboldt Univ, Germany; Berlin Inst Hlth, Germany.
    Haviland, David L.
    Houston Methodist Hosp Res Inst, TX USA.
    Hedley, David
    Princess Margaret Hosp, Canada.
    Hernandez, Daniela C.
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Herrera, Guadalupe
    Univ Valencia, Spain; Univ Valencia, Spain.
    Herrmann, Martin
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Hess, Christoph
    Univ Basel, Switzerland; Univ Hosp Basel, Switzerland; Univ Cambridge, England.
    Hoefer, Thomas
    German Canc Res Ctr, Germany.
    Hoffmann, Petra
    Regensburg Ctr Intervent Immunol RCI, Germany; Univ Hosp Regensburg, Germany.
    Hogquist, Kristin
    Univ Minnesota, MN USA.
    Holland, Tristan
    Univ Bonn, Germany.
    Hoellt, Thomas
    Leiden Univ, Netherlands; Delft Univ Technol, Netherlands.
    Holmdahl, Rikard
    Karolinska Inst, Sweden.
    Hombrink, Pleun
    Univ Amsterdam, Netherlands; Sanquin Res, Netherlands; Univ Amsterdam, Netherlands.
    Houston, Jessica P.
    New Mexico State Univ, NM 88003 USA.
    Hoyer, Bimba F.
    Univ Klinikum Schleswig Holstein, Germany; Univ Klinikum Schleswig Holstein, Germany.
    Huang, Bo
    Chinese Acad Med Sci and Peking Union Med Coll, Peoples R China; Chinese Acad Med Sci and Peking Union Med Coll, Peoples R China.
    Huang, Fang-Ping
    Shenzhen Univ, Peoples R China.
    Huber, Johanna E.
    Ludwig Maximilians Univ Munchen, Germany.
    Huehn, Jochen
    Helmholtz Ctr Infect Res, Germany.
    Hundemer, Michael
    Heidelberg Univ, Germany.
    Hunter, Christopher A.
    Univ Penn, PA 19104 USA.
    Hwang, William Y. K.
    Singapore Gen Hosp, Singapore; Duke NUS Med Sch, Singapore; Natl Canc Ctr Singapore, Singapore.
    Iannone, Anna
    Univ Modena and Reggio Emilia, Italy.
    Ingelfinger, Florian
    Univ Zurich, Switzerland.
    Ivison, Sabine M.
    Univ British Columbia, Canada; BC Childrens Hosp Res Inst, Canada.
    Jaeck, Hans-Martin
    Univ Erlangen Nurnberg, Germany.
    Jani, Peter K.
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Max Planck Inst Infect Biol, Germany.
    Javega, Beatriz
    Univ Valencia, Spain.
    Jonjic, Stipan
    Univ Rijeka, Croatia.
    Kaiser, Toralf
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Kalina, Tomas
    Charles Univ Prague, Czech Republic.
    Kamradt, Thomas
    Jena Univ Hosp, Germany.
    Kaufmann, Stefan H. E.
    Max Planck Inst Infect Biol, Germany.
    Keller, Baerbel
    Univ Freiburg, Germany; Univ Freiburg, Germany.
    Ketelaars, Steven L. C.
    Netherlands Canc Inst, Netherlands.
    Khalilnezhad, Ahad
    ASTAR, Singapore; Natl Univ Singapore, Singapore.
    Khan, Srijit
    Univ Toronto, Canada.
    Kisielow, Jan
    Swiss Fed Inst Technol, Switzerland.
    Klenerman, Paul
    Univ Oxford, England.
    Knopf, Jasmin
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Koay, Hui-Fern
    Univ Melbourne, Australia.
    Kobow, Katja
    Univ Klinikum Erlangen, Germany.
    Kolls, Jay K.
    Tulane Sch Med, LA USA.
    Kong, Wan Ting
    ASTAR, Singapore.
    Kopf, Manfred
    Swiss Fed Inst Technol, Switzerland.
    Korn, Thomas
    Tech Univ Munich, Germany.
    Kriegsmann, Katharina
    Heidelberg Univ, Germany.
    Kristyanto, Hendy
    Leiden Univ, Netherlands.
    Kroneis, Thomas
    Med Univ Graz, Austria.
    Krueger, Andreas
    Goethe Univ Frankfurt, Germany.
    Kuehne, Jenny
    Hannover Med Sch, Germany.
    Kukat, Christian
    Max Planck Inst Biol Ageing, Germany; Max Planck Inst Biol Ageing, Germany.
    Kunkel, Desiree
    Charite Univ Med Berlin, Germany; Berlin Inst Hlth, Germany; Charite Univ Med Berlin, Germany.
    Kunze-Schumacher, Heike
    Goethe Univ Frankfurt, Germany.
    Kurosaki, Tomohiro
    Osaka Univ, Japan.
    Kurts, Christian
    Univ Bonn, Germany.
    Kvistborg, Pia
    Netherlands Canc Inst, Netherlands.
    Kwok, Immanuel
    ASTAR, Singapore; Nanyang Technol Univ, Singapore.
    Landry, Jonathan
    EMBL, Germany.
    Lantz, Olivier
    PSL Univ, France.
    Lanuti, Paola
    Univ G dAnnunzio, Italy.
    LaRosa, Francesca
    IRCCS Fdn Don Carlo Gnocchi, Italy; Univ Milano Bicocca, Italy.
    Lehuen, Agnes
    Univ Paris, France.
    LeibundGut-Landmann, Salome
    Univ Zurich, Switzerland.
    Leipold, Michael D.
    Stanford Univ, CA 94305 USA.
    Leung, Leslie Y. T.
    Univ Toronto, Canada.
    Levings, Megan K.
    Univ British Columbia, Canada; BC Childrens Hosp Res Inst, Canada; Univ British Columbia, Canada.
    Lino, Andreia C.
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Liotta, Francesco
    Univ Florence, Italy.
    Litwin, Virginia
    ImmuneCarta, Canada.
    Liu, Yanling
    Univ Toronto, Canada.
    Ljunggren, Hans-Gustaf
    Karolinska Inst, Sweden.
    Lohoff, Michael
    Univ Marburg, Germany.
    Lombardi, Giovanna
    Kings Coll London, England.
    Lopez, Lilly
    Beckman Coulter Inc, FL USA.
    Lopez-Botet, Miguel
    Univ Pompeu Fabra, Spain.
    Lovett-Racke, Amy E.
    Ohio State Univ, OH 43210 USA.
    Lubberts, Erik
    Univ Med Ctr Rotterdam, Netherlands.
    Luche, Herve
    Aix Marseille Univ, France.
    Ludewig, Burkhard
    Kantonsspital St Gallen, Switzerland.
    Lugli, Enrico
    Humanitas Clin and Res Ctr, Italy; Humanitas Clin and Res Ctr, Italy.
    Lunemann, Sebastian
    Leibniz Inst Expt Virol, Germany.
    Maecker, Holden T.
    Stanford Univ, CA 94305 USA.
    Maggi, Laura
    Univ Florence, Italy.
    Maguire, Orla
    Roswell Park Comprehens Canc Ctr, NY USA.
    Mair, Florian
    Fred Hutchinson Canc Res Ctr, WA 98104 USA.
    Mair, Kerstin H.
    Univ Vet Med Vienna, Austria; Univ Vet Med Vienna, Austria.
    Mantovani, Alberto
    Ist Clin Humanitas IRCCS, Italy; Humanitas Univ, Italy; Queen Mary Univ, England.
    Manz, Rudolf A.
    Univ Lubeck, Germany.
    Marshall, Aaron J.
    Univ Manitoba, Canada.
    Martinez-Romero, Alicia
    Principe Felipe Res Ctr, Spain.
    Martrus, Gloria
    Leibniz Inst Expt Virol, Germany.
    Marventano, Ivana
    IRCCS Fdn Don Carlo Gnocchi, Italy; Univ Milano Bicocca, Italy.
    Maslinski, Wlodzimierz
    Natl Inst Geriatr Rheumatol and Rehabil, Poland.
    Matarese, Giuseppe
    Univ Napoli Federico II, Italy; CNR, Italy.
    Mattioli, Anna Vittoria
    Univ Modena and Reggio Emilia, Italy; Humanitas Clin and Res Ctr, Italy.
    Maueroeder, Christian
    VIB Ctr Inflammat Res, Belgium; Univ Ghent, Belgium.
    Mazzoni, Alessio
    Univ Florence, Italy.
    McCluskey, James
    Univ Melbourne, Australia.
    McGrath, Mairi
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    McGuire, Helen M.
    Univ Sydney, Australia; Univ Sydney, Australia.
    McInnes, Iain B.
    Univ Glasgow, Scotland.
    Mei, Henrik E.
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Melchers, Fritz
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Max Planck Inst Infect Biol, Germany.
    Melzer, Susanne
    Univ Leipzig, Germany.
    Mielenz, Dirk
    Univ Erlangen Nurnberg, Germany.
    Miller, Stephen D.
    Northwestern Univ, IL USA.
    Mills, Kingston H. G.
    Trinity Coll Dublin, Ireland.
    Minderman, Hans
    Roswell Park Comprehens Canc Ctr, NY USA.
    Mjösberg, Jenny
    Linköpings universitet, Institutionen för klinisk och experimentell medicin. Linköpings universitet, Medicinska fakulteten. Karolinska Inst, Sweden.
    Moore, Jonni
    Univ Penn, PA 19104 USA.
    Moran, Barry
    Trinity Coll Dublin, Ireland.
    Moretta, Lorenzo
    IRCCS Bambino Gesu Childrens Hosp, Italy.
    Mosmann, Tim R.
    Univ Rochester, NY 14642 USA.
    Mueller, Susann
    UFZ Helmholtz Ctr Environm Res, Germany.
    Multhoff, Gabriele
    Helmholtz Zentrum Munchen, Germany; Tech Univ Munchen TranslaTUM, Germany.
    Munoz, Luis Enrique
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Muenz, Christian
    Univ Zurich, Switzerland; Comprehens Canc Ctr Zurich, Switzerland.
    Nakayama, Toshinori
    Chiba Univ, Japan.
    Nasi, Milena
    Univ Modena and Reggio Emilia, Italy.
    Neumann, Katrin
    Univ Med Ctr Hamburg Eppendorf, Germany.
    Ng, Lai Guan
    ASTAR, Singapore; Natl Univ Singapore, Singapore; Nanyang Technol Univ, Singapore; Univ Sydney, Australia; Chinese Acad Med Sci and Peking Union Med Coll, Peoples R China.
    Niedobitek, Antonia
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Nourshargh, Sussan
    Queen Mary Univ London, England.
    Nunez, Gabriel
    Univ Michigan, MI 48109 USA; Univ Michigan, MI 48109 USA.
    OConnor, Jose-Enrique
    Univ Valencia, Spain.
    Ochel, Aaron
    Univ Med Ctr Hamburg Eppendorf, Germany.
    Oja, Anna
    Sanquin Res, Netherlands; Univ Amsterdam, Netherlands.
    Ordonez, Diana
    EMBL, Germany.
    Orfao, Alberto
    Univ Salamanca, Spain; Inst Biomed Res Salamanca IBSAL, Spain.
    Orlowski-Oliver, Eva
    Burnet Inst, Australia.
    Ouyang, Wenjun
    Amgen Inc, CA USA.
    Oxenius, Annette
    Swiss Fed Inst Technol, Switzerland.
    Palankar, Raghavendra
    Univ Med Greifswald, Germany.
    Panse, Isabel
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Pattanapanyasat, Kovit
    Mahidol Univ, Thailand.
    Paulsen, Malte
    EMBL, Germany.
    Pavlinic, Dinko
    EMBL, Germany.
    Penter, Livius
    Charite Univ Med Berlin, Germany.
    Peterson, Paert
    Univ Tartu, Estonia.
    Peth, Christian
    Miltenyi Biotec GmbH, Germany.
    Petriz, Jordi
    UAB, Spain.
    Piancone, Federica
    IRCCS Fdn Don Carlo Gnocchi, Italy; Univ Milano Bicocca, Italy.
    Pickl, Winfried F.
    Med Univ Vienna, Austria.
    Piconese, Silvia
    Sapienza Univ Roma, Italy; Fdn Cenci Bolognetti, Italy.
    Pinti, Marcello
    Univ Modena and Reggio Emilia, Italy.
    Pockley, A. Graham
    Nottingham Trent Univ, England; Chromocyte Ltd, England.
    Podolska, Malgorzata Justyna
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany; Univ Klinikum Erlangen, Germany.
    Poon, Zhiyong
    Singapore Gen Hosp, Singapore.
    Pracht, Katharina
    Univ Erlangen Nurnberg, Germany.
    Prinz, Immo
    Hannover Med Sch, Germany.
    Pucillo, Carlo E. M.
    Univ Udine, Italy.
    Quataert, Sally A.
    Univ Rochester, NY 14642 USA.
    Quatrini, Linda
    IRCCS Bambino Gesu Childrens Hosp, Italy.
    Quinn, Kylie M.
    RMIT Univ, Australia; Monash Univ, Australia.
    Radbruch, Helena
    Charite Univ Med Berlin, Germany; Free Univ Berlin, Germany; Humboldt Univ, Germany; Berlin Inst Hlth, Germany.
    Radstake, Tim R. D. J.
    Univ Utrecht, Netherlands.
    Rahmig, Susann
    Fritz Lipmann Inst FLI, Germany.
    Rahn, Hans-Peter
    Max Delbruck Ctr Mol Med, Germany.
    Rajwa, Bartek
    Purdue Univ, IN 47907 USA.
    Ravichandran, Gevitha
    Univ Med Ctr Hamburg Eppendorf, Germany.
    Raz, Yotam
    Groene Hart Hosp, Netherlands.
    Rebhahn, Jonathan A.
    Univ Rochester, NY 14642 USA.
    Recktenwald, Diether
    Desatoya LLC, NV USA.
    Reimer, Dorothea
    Univ Erlangen Nurnberg, Germany.
    Reis e Sousa, Caetano
    Francis Crick Inst, England.
    Remmerswaal, Ester B. M.
    Univ Amsterdam, Netherlands; Univ Amsterdam, Netherlands.
    Richter, Lisa
    Ludwig Maximilians Univ Munchen, Germany.
    Rico, Laura G.
    UAB, Spain.
    Riddell, Andy
    Francis Crick Inst, England.
    Rieger, Aja M.
    Univ Alberta, Canada.
    Robinson, J. Paul
    Purdue Univ, IN 47907 USA.
    Romagnani, Chiara
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Rubartelli, Anna
    IRCCS Osped Policlin San Martino, Italy.
    Ruland, Juergen
    Tech Univ Munich, Germany.
    Saalmueller, Armin
    Univ Vet Med Vienna, Austria.
    Saeys, Yvan
    VIB UGent Ctr Inflammat Res, Belgium; Univ Ghent, Belgium.
    Saito, Takashi
    RIKEN Ctr Integrat Med Sci, Japan.
    Sakaguchi, Shimon
    Osaka Univ, Japan.
    Sala-de-Oyanguren, Francisco
    Univ Lausanne, Switzerland.
    Samstag, Yvonne
    Heidelberg Univ, Germany.
    Sanderson, Sharon
    Univ Oxford, England.
    Sandrock, Inga
    Hannover Med Sch, Germany.
    Santoni, Angela
    Sapienza Univ Rome, Italy.
    Sanz, Ramon Bellmas
    Hannover Med Sch, Germany.
    Saresella, Marina
    IRCCS Fdn Don Carlo Gnocchi, Italy; Univ Milano Bicocca, Italy.
    Sautes-Fridman, Catherine
    Ctr Rech Cordeliers, France.
    Sawitzki, Birgit
    Charite Univ Med Berlin, Germany; Berlin Inst Hlth, Germany.
    Schadt, Linda
    Univ Zurich, Switzerland; Comprehens Canc Ctr Zurich, Switzerland.
    Scheffold, Alexander
    Christian Albrechts Univ Kiel, Germany.
    Scherer, Hans U.
    Leiden Univ, Netherlands.
    Schiemann, Matthias
    Tech Univ Munich, Germany.
    Schildberg, Frank A.
    Univ Hosp Bonn, Germany.
    Schimisky, Esther
    Miltenyi Biotec GmbH, Germany.
    Schlitzer, Andreas
    Univ Bonn, Germany.
    Schlosser, Josephine
    Free Univ Berlin, Germany.
    Schmid, Stephan
    Univ Hosp Regensburg, Germany.
    Schmitt, Steffen
    German Canc Res Ctr, Germany.
    Schober, Kilian
    Tech Univ Munich, Germany.
    Schraivogel, Daniel
    EMBL, Germany.
    Schuh, Wolfgang
    Univ Erlangen Nurnberg, Germany.
    Schueler, Thomas
    Otto von Guericke Univ, Germany.
    Schulte, Reiner
    Univ Cambridge, England.
    Schulz, Axel Ronald
    Deutsch Rheuma Forschungszentrum DRFZ, Germany.
    Schulz, Sebastian R.
    Univ Erlangen Nurnberg, Germany.
    Scotta, Cristiano
    Kings Coll London, England.
    Scott-Algara, Daniel
    Inst Pasteur, France.
    Sester, David P.
    Translat Res Inst, Australia.
    Shankey, T. Vincent
    AsedaSciences, IN USA.
    Silva-Santos, Bruno
    Univ Lisbon, Portugal.
    Simon, Anna Katharina
    Univ Oxford, England.
    Sitnik, Katarzyna M.
    Helmholtz Ctr Infect Res, Germany.
    Sozzani, Silvano
    Univ Brescia, Italy.
    Speiser, Daniel E.
    Univ Lausanne, Switzerland.
    Spidlen, Josef
    BD Life Sci, OR USA.
    Stahlberg, Anders
    Univ Gothenburg, Sweden.
    Stall, Alan M.
    BD Life Sci, CA USA.
    Stanley, Natalie
    Stanford Univ, CA 94305 USA; Stanford Univ, CA 94305 USA; Stanford Univ, CA 94305 USA.
    Stark, Regina
    Univ Amsterdam, Netherlands; Sanquin Res, Netherlands; Univ Amsterdam, Netherlands.
    Stehle, Christina
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Steinmetz, Tobit
    Univ Erlangen Nurnberg, Germany.
    Stockinger, Hannes
    Med Univ Vienna, Austria.
    Takahama, Yousuke
    NIH, MD 20892 USA.
    Takeda, Kiyoshi
    Osaka Univ, Japan.
    Tan, Leonard
    ASTAR, Singapore; Natl Univ Singapore, Singapore.
    Tarnok, Attila
    Fraunhofer Inst Cell Therapy and Immunol IZI, Germany; Univ Leipzig, Germany; Tsinghua Univ, Peoples R China.
    Tiegs, Gisa
    Univ Med Ctr Hamburg Eppendorf, Germany.
    Toldi, Gergely
    MDQuest Ltd, Hungary.
    Tornack, Julia
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; BioGenes GmbH, Germany.
    Traggiai, Elisabetta
    NIBR, Switzerland.
    Trebak, Mohamed
    Penn State Univ, PA 16802 USA.
    Tree, Timothy I. M.
    Kings Coll London, England; Guys and St Thomas Natl Hlth Serv Fdn Trust, England; Kings Coll London, England.
    Trotter, Joe
    BD Life Sci, CA USA.
    Trowsdale, John
    Univ Cambridge, England.
    Tsoumakidou, Maria
    BSRC Alexander Fleming, Greece.
    Ulrich, Henning
    Univ Sao Paulo, Brazil.
    Urbanczyk, Sophia
    Univ Erlangen Nurnberg, Germany.
    van de Veen, Willem
    Univ Zurich, Switzerland; Christine Kuhne Ctr Allergy Res and Educ CK CARE, Switzerland.
    van den Broek, Maries
    Univ Zurich, Switzerland; Comprehens Canc Ctr Zurich, Switzerland.
    van der Pol, Edwin
    Univ Amsterdam, Netherlands.
    Van Gassen, Sofie
    VIB UGent Ctr Inflammat Res, Belgium; Univ Ghent, Belgium.
    Van Isterdael, Gert
    VIB Ctr Inflammat Res, Belgium.
    van Lier, Rene A. W.
    Sanquin Res, Netherlands; Univ Amsterdam, Netherlands.
    Veldhoen, Marc
    Univ Lisbon, Portugal.
    Vento-Asturias, Salvador
    Univ Bonn, Germany.
    Vieira, Paulo
    Inst Pasteur, France.
    Voehringer, David
    Friedrich Alexander Univ Erlangen Nurnberg FAU, Germany.
    Volk, Hans-Dieter
    Charite Univ Med Berlin, Germany; Berlin Inst Hlth, Germany.
    von Borstel, Anouk
    Monash Univ, Australia; Monash Univ, Australia; Monash Univ, Australia.
    von Volkmann, Konrad
    APE Appl Phys and Elect, Germany.
    Waisman, Ari
    Johannes Gutenberg Univ Mainz, Germany.
    Walker, Rachael V.
    Babraham Inst, England.
    Wallace, Paul K.
    Roswell Park Comprehens Canc Ctr, NY USA.
    Wang, Sa A.
    Univ Texas MD Anderson Canc Ctr, TX 77030 USA.
    Wang, Xin M.
    Westmead Res Hub, Australia.
    Ward, Michael D.
    Thermo Fisher Sci, OR USA.
    Ward-Hartstonge, Kirsten A.
    Univ British Columbia, Canada.
    Warnatz, Klaus
    Univ Freiburg, Germany; Univ Freiburg, Germany.
    Warnes, Gary
    Queen Mary Univ London, England.
    Warth, Sarah
    Charite Univ Med Berlin, Germany.
    Waskow, Claudia
    Fritz Lipmann Inst FLI, Germany; Friedrich Schiller Univ Jena, Germany.
    Watson, James V.
    Medinfomatics Ltd, England.
    Watzl, Carsten
    TU Dortmund IfADo, Germany.
    Wegener, Leonie
    Miltenyi Biotec GmbH, Germany.
    Weisenburger, Thomas
    Friedrich Alexander Univ Erlangen Nuremberg, Germany.
    Wiedemann, Annika
    Deutsch Rheuma Forschungszentrum DRFZ, Germany; Charite Univ Med Berlin, Germany.
    Wienands, Juergen
    Univ Med Ctr Gottingen, Germany.
    Wilharm, Anneke
    Hannover Med Sch, Germany.
    Wilkinson, Robert John
    Imperial Coll London, England; Univ Cape Town, South Africa; Univ Cape Town, South Africa; Francis Crick Inst, England.
    Willimsky, Gerald
    Charite Univ Med Berlin, Germany; German Canc Res Ctr, Germany.
    Wing, James B.
    Osaka Univ, Japan.
    Winkelmann, Rieke
    Christian Albrechts Univ Kiel, Germany.
    Winkler, Thomas H.
    Friedrich Alexander Univ Erlangen Nuremberg, Germany.
    Wirz, Oliver F.
    Univ Zurich, Switzerland.
    Wong, Alicia
    ASTAR, Singapore.
    Wurst, Peter
    Univ Bonn, Germany.
    Yang, Jennie H. M.
    Kings Coll London, England; Guys and St Thomas Natl Hlth Serv Fdn Trust, England; Kings Coll London, England.
    Yang, Juhao
    Helmholtz Ctr Infect Res, Germany.
    Yazdanbakhsh, Maria
    Leiden Univ, Netherlands.
    Yu, Liping
    BD Biosci, CA USA.
    Yue, Alice
    Simon Fraser Univ, Canada.
    Zhang, Hanlin
    Univ Oxford, England.
    Zhao, Yi
    Sichuan Univ, Peoples R China.
    Ziegler, Susanne Maria
    Leibniz Inst Expt Virol, Germany.
    Zielinski, Christina
    German Ctr Infect Res DZIF, Germany; Tech Univ Munich, Germany; Tech Univ Munich, Germany.
    Zimmermann, Jakob
    Univ Bern, Switzerland.
    Zychlinsky, Arturo
    Max Planck Inst Infect Biol, Germany.
    Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)2019Ingår i: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 49, nr 10, s. 1457-1973Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

  • 331.
    Cranford, Ted W.
    et al.
    San Diego State University, USA.
    Amundin, Mats
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Krysl, Petr
    University of California, San Diego, USA.
    Sound production and sound reception in Delphinoids2015Ingår i: Dolphin communication and cognition: past, present, and future / [ed] Denise L. Herzing and Christine M. Johnson, Cambridge: MIT Press, 2015, s. 19-48Kapitel i bok, del av antologi (Övrigt vetenskapligt)
    Abstract [en]

    Book Abstract

    Dolphin researchers have collected an impressive amount of data over the last twenty years, thanks to advances in technology for monitoring, recording, and analyzing dolphin behavior as well as increasing interest in exploring and modeling dolphins’ cognitive capacities. This volume offers a comprehensive reference to the latest research on dolphin communication and cognition, reporting on findings from both the laboratory and the field. The contributors review a wide range of topics, including vocalization, abstract reasoning abilities, imitation and learning, social cognition, echolocation, and ethical issues in working with cetaceans.The book begins by examining the dolphin brain and its evolution, the anatomy of its unique sound production and reception systems, and its sensory abilities. It next treats communication, reviewing the complexity of dolphins’ vocalization, and then describes research on cognition, from both experimental and developmental perspectives. Finally, the book considers the future of dolphin research, including a series of provocative questions that remain unanswered, posed by the volume’s expert contributors.

  • 332.
    Crespo Mingueza, Laia
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi.
    Assessment of lateralized behaviour in free-ranging Mexican mantled howler monkeys (Alouatta palliata mexicana)2015Självständigt arbete på avancerad nivå (masterexamen), 40 poäng / 60 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The evolutionary origins of human handedness are still unknown. The study of lateralized behaviour in our closest relatives, the nonhuman primates, is useful to clarify how this trait appeared and evolved in our species. In the present study, lateralized behaviour was assessed in a population of 32 free-ranging Mexican mantled howler monkeys (Alouatta palliata mexicana) for thirteen spontaneous motor patterns, at individual and group levels, as well as the effect that age, sex and posture have on its strength and direction. The studied population of howler monkeys displayed only few significant lateral biases at the individual level with single motor patterns (Binomial tests, p≤0.05). No biases towards the use of a particular limb or side of the body were found at a population level. Therefore, even though some individuals showed significant limb/side preference with single motor patterns, no signs of task specialization, side specialization, or true handedness were found. Similarly, no effects of sex, age or posture were found on the direction or strength of lateralized behaviour. The general absence of limb/side preferences found in this population may be due to the constraints imposed by the arboreal life and/or the type of diet. Possible causal agents of the few significant individual biases found here may be the presence of handicaps and/or experience. Further research is needed in order to assess whether the lack of human-like handedness reported in this study is only specific to the studied population, a general phenomenon of the genus Alouatta or perhaps of all the Platyrrhini.

  • 333.
    Cristobal, Susana
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Tedesco, Sara
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för cellbiologi. Linköpings universitet, Hälsouniversitetet.
    Bayat, Narges
    Stockholm University.
    Danielsson, Gabriela
    Stockholm University.
    Buque, Xavier
    Basque country University, Spain.
    Aspichueta, Patricia
    Basque Country University, Spain.
    Fresnedo, Olatz
    Basque Country University, Spain.
    Proteomic and lipidomic analysis of primary mouse hepatocytes exposed to metal and metal oxide nanoparticles2015Ingår i: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 5, nr 1, s. 44-57Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The global analysis of the cellular lipid and protein content upon exposure to metal and metal oxide nanoparticles (NPs) can provide an overviewof the possible impact of exposure. Proteomic analysis has been applied to understand the nanoimpact however the relevance of the alterationon the lipidic proOle has been underestimated. In our study, primary mouse hepatocytes were treated with ultra-small (US) TiO2-USNPsas well as ZnO-NPs, CuO-NPs and Ag-NPs. e protein extracts were analysed by 2D-DIGE and quantiOed by imaging soPware and the selecteddi9erentially expressed proteins were identiOed by nLC-ESI-MS/MS. In parallel, lipidomic analysis of the samples was performed usingthin layer chromatography (TLC) and analyzed by imaging soPware. Our Ondings show an overall ranking of the nanoimpact at the cellularand molecular level: TiO2-USNPs<ZnO-NPs<Ag-NPs<CuO-NPs. CuO-NPs and Ag-NPs were cytotoxic while ZnO-NPs and CuO-NPs hadoxidative capacity. TiO2-USNPs did not have oxidative capacity and were not cytotoxic. e most common cellular impact of the exposurewas the down-regulation of proteins. e proteins identiOed were involved in urea cycle, lipid metabolism, electron transport chain, metabolismsignaling, cellular structure and we could also identify nuclear proteins. CuO-NPs exposure decreased phosphatidylethanolamine andphosphatidylinositol and caused down-regulation of electron transferring protein subunit beta. Ag-NPs exposure caused increased of totallipids and triacylglycerol and decrease of sphingomyelin. TiO2-USNPs also caused decrease of sphingomyelin as well as up-regulation of ATPsynthase and electron transferring protein alfa. ZnO-NPs a9ected the proteome in a concentration-independent manner with down-regulationof RNA helicase. ZnO-NPs exposure did not a9ect the cellular lipids. To our knowledge this work represents the Orst integrated proteomic andlipidomic approach to study the e9ect of NPs exposure to primary mouse hepatocytes in vitro.

  • 334.
    Crossley, Dane A
    et al.
    Department of Biological Sciences , University of North Texas Denton, Texas, USA.
    Burggren, Warren W
    Department of Biological Sciences , University of North Texas Denton, Texas, USA.
    Reiber, Carl L
    School of Life Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, USA.
    Altimiras, Jordi
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Rodnick, Kenneth J
    Department of biological Sceinces, Idaho State UNiversity, Pocatello; Idaho, USA.
    Mass Transport: Circulatory System with Emphasis on Nonendothermic Species.2017Ingår i: Comprehensive Physiology, ISSN 0706-1994, E-ISSN 2165-2228, Vol. 7, nr 1, s. 17-66Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Mass transport can be generally defined as movement of material matter. The circulatory system then is a biological example given its role in the movement in transporting gases, nutrients, wastes, and chemical signals. Comparative physiology has a long history of providing new insights and advancing our understanding of circulatory mass transport across a wide array of circulatory systems. Here we focus on circulatory function of nonmodel species. Invertebrates possess diverse convection systems; that at the most complex generate pressures and perform at a level comparable to vertebrates. Many invertebrates actively modulate cardiovascular function using neuronal, neurohormonal, and skeletal muscle activity. In vertebrates, our understanding of cardiac morphology, cardiomyocyte function, and contractile protein regulation by Ca2+ highlights a high degree of conservation, but differences between species exist and are coupled to variable environments and body temperatures. Key regulators of vertebrate cardiac function and systemic blood pressure include the autonomic nervous system, hormones, and ventricular filling. Further chemical factors regulating cardiovascular function include adenosine, natriuretic peptides, arginine vasotocin, endothelin 1, bradykinin, histamine, nitric oxide, and hydrogen sulfide, to name but a few. Diverse vascular morphologies and the regulation of blood flow in the coronary and cerebral circulations are also apparent in nonmammalian species. Dynamic adjustments of cardiovascular function are associated with exercise on land, flying at high altitude, prolonged dives by marine mammals, and unique morphology, such as the giraffe. Future studies should address limits of gas exchange and convective transport, the evolution of high arterial pressure across diverse taxa, and the importance of the cardiovascular system adaptations to extreme environments. © 2017 American Physiological Society. Compr Physiol 7:17-66, 2017.

  • 335.
    Csizmok, Veronika
    et al.
    Hospital Sick Children, Canada; University of British Columbia, Canada.
    Montecchio, Meri
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska fakulteten.
    Lin, Hong
    Hospital Sick Children, Canada.
    Tyers, Mike
    University of Montreal, Canada.
    Sunnerhagen, Maria
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Kemi. Linköpings universitet, Tekniska fakulteten.
    Forman-Kay, Julie D.
    Hospital Sick Children, Canada; University of Toronto, Canada.
    Multivalent Interactions with Fbw7 and Pin1 Facilitate Recognition of c-Jun by the SCFFbw7 Ubiquitin Ligase2018Ingår i: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 26, nr 1, s. 28-+Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Many regulatory proteins, including the transcription factor c-Jun, are highly enriched in disordered protein regions that govern growth, division, survival, differentiation, and response to signals. The stability of c-Jun is controlled by poorly understood regulatory interactions of its disordered region with both the E3 ubiquitin ligase SCFFbw7 and prolyl cis-trans isomerase Pin1. We use nuclear magnetic resonance and fluorescence studies of c-Jun to demonstrate that multisite c-Jun phosphorylation is required for high-affinity interaction with Fbw7. We show that the Pin1 WW and PPIase domains interact in a dynamic complex with multiply phosphorylated c-Jun. Importantly, Pin1 isomerizes a pSer-Pro peptide bond at the c-Jun N terminus that affects binding to Fbw7 and thus modulates the ubiquitin-mediated degradation of c-Jun. Our findings support the general principle that multiple weak binding motifs within disordered regions can synergize to yield high-affinity interactions and provide rapidly evolvable means to build and fine-tune regulatory events.

  • 336.
    Curtsdotter, Alva
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska högskolan.
    Binzer, Amrei
    J.F. Blumenbach Institute of Zoology and Anthropology, Georg-August University, Göttingen, Germany.
    Brose, Ulrich
    J.F. Blumenbach Institute of Zoology and Anthropology, Georg-August University, Göttingen, Germany.
    Ebenman, Bo
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska högskolan.
    The interaction between species traits and community properties determine food web resistance to species loss2014Manuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    The ability to identify the ecosystems most vulnerable to species loss is fundamental for the allocation of conservation efforts. With this aim, the traits of keystone species have been investigated, as have the properties defining systems especially sensitive to species loss. However, these two have rarely been investigated in relation to each other. Here we show, that the traits of the species primarily lost act in conjunction with the properties of the food web from which it is lost, in determining the resistance of the system. We find that the extent of bottom-up extinction cascades is determined mainly by traits related to food web topology, while traits related to population dynamics govern the extent of top-down cascades. As different disturbances affect species with different traits, this interaction implies that the characteristics defining a sensitive community depend on the disturbance it is subjected to.

  • 337.
    Curtsdotter, Alva
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska högskolan.
    Münger, Peter
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Fysik. Linköpings universitet, Tekniska högskolan.
    Norberg, Jon
    Department of Systems Ecology, Stockholm University/Stockholm Resilience Centre, Stockholm University, Sweden.
    Åkesson, Anna
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Ebenman, Bo
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teoretisk Biologi. Linköpings universitet, Tekniska högskolan.
    The strength of interspecific competition modulates the eco-evolutionary response to climate change2014Manuskript (preprint) (Övrigt vetenskapligt)
    Abstract [en]

    Climate change is predicted to have major implications for global biodiversity. Dispersal and evolution may become crucial for species survival, as species must either adapt or migrate to track the changing climate. However, migration and evolution do not occur in vacuum – the biotic community in which these processes play out may modulate their effect on biodiversity. Here, we use an eco-evolutionary, spatially explicit, multi-species model that allows us to examine the interactive effects of competition, adaptation and dispersal on species richness in plant communities under global warming. We find that there is a larger decline in global species richness when interspecific competition is strong. Furthermore, there is a three-way interaction between interspecific competition, evolution and dispersal that creates a complex pattern of biodiversity responses, in which both evolution and dispersal can either increase or decrease the magnitude of species loss. This interaction arises for at least two reasons: 1) different levels of dispersal, evolution and competition creates differences in local and global community structure before climate change, and 2) competitive interactions determine whether the benefits of dispersal and/or evolution (climate tracking and adaptation) outweighs the risks (competitive exclusion).

  • 338.
    Dahl, Kristoffer
    Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Management effects on butterfly and bumblebee abundance in Swedish semi-natural grasslands2015Självständigt arbete på grundnivå (kandidatexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [en]

    The amount of semi-natural grasslands has decreased in the agricultural landscape and because of this many grassland species are declining. Semi-natural grasslands are dependent on management, but different studies suggest that different management methods are most favorable to species richness and abundance. The aim of this study was to compare the effects of mowing and grazing on butterfly and bumblebee abundance in Swedish semi-natural grasslands. We used data collected through the Swedish environmental monitoring program NILS. We identified 31 comparable meadows and chose 1-10 pastures in the area surrounding each meadow. For six different species groups we calculated the number of individuals found per 100 m. By standardizing the values of the difference between the means for meadows and pastures we were able to compare the effects of the two methods on abundance in each species group. No species group showed a preference for pastures. A significant preference for meadows was found for two butterfly species groups. The four other groups showed a trend to prefer meadows before pastures. According to our study the recommended management method should be mowing, considering protection of butterflies and bumblebees.

  • 339.
    Dahlerup, Nina
    Linköpings universitet, Institutionen för fysik, kemi och biologi.
    Effects of site quality and surrounding landscape on bryophytes and brackets on logs in woodland key habitats2010Självständigt arbete på avancerad nivå (magisterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
    Abstract [en]

    A tool for management and conservation of valuable forests in Sweden are WKH:s. In this study WKH:s different in size, connectivity, amount of dead wood and quality of logs were investigated for species richness of bryophytes and brackets on coniferous logs. The aim was to clarify which scales and features that was important for the diversity of species as well as for individual species. The results showed that the amount of dead wood was most important on the site scale, and some species were affected at the landscape scale, a positive effect of valuable tracts. On the scale of individual logs, factors such as diameter, sun exposure, succession stage, contact with ground and ground bryophytes cover was most important. Red-listed species preferred logs with large diameter and late successional stages. The conclusion was that the quality of the substrate and the amount of dead wood was most important, but the amount of WKH:s on the landscape scale was also important for some species.

  • 340.
    Dalbato, A. L.
    et al.
    Swedish University of Agriculture Science, Sweden .
    Alfredsson, T.
    Swedish University of Agriculture Science, Sweden .
    Karlsson, L. M.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska högskolan. Swedish Univ Agr Sci, Sweden.
    Andersson, L.
    Swedish University of Agriculture Science, Sweden .
    Effect of rhizome fragment length and burial depth on emergence of Tussilago farfara2014Ingår i: Weed research (Print), ISSN 0043-1737, E-ISSN 1365-3180, Vol. 54, nr 4, s. 347-355Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Mechanical control of Tussilago farfara is carried out mainly by soil cultivation. The aim is to deplete the energy stored in the rhizomes. The treatment includes cutting the rhizomes, to stimulate increased shooting, followed by renewed soil cultivation to destroy the shoots and incorporate them into the soil. Factors generally regarded as important in the control of perennial weeds are extent of fragmentation and burial depth. In this study, the importance of these two factors on T. farfara emergence was studied in detail in two pot experiments. Rhizomes were cut into different lengths (5-25 cm) and buried at various depths (1-42 cm) in pots filled with peat soil or clay loam. Shoot germination, emergence and early plant performance were studied. Intensive fragmentation and deep burial (possible to achieve using conventional tillage) are not enough to completely hinder emergence of T. farfara; 6-cm fragments emerged and developed normal leaves from 42 cm depth, regardless of soil type. However, there were higher total emergence and emergence rates in peat soil than in clay soil. Burial depth was correlated with time to emergence; burying rhizome fragments, not longer than 25 cm, to at least 20 cm depth gave a time to emergence of at least 20 days. The delay of weed emergence should allow good establishment of a crop and ensure a significant competitive effect against T. farfara.

  • 341.
    Danielsson, Elsa
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi.
    Carlsson, Evelina
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi.
    En jämförande studie av skogsstrukturer i olika skogstyper i Östergötland: – och ett förslag på hur ett biologiprojekt som behandlar detta kan genomföras i gymnasiekursen Biologi 12017Självständigt arbete på avancerad nivå (yrkesexamen), 10 poäng / 15 hpStudentuppsats (Examensarbete)
    Abstract [sv]

    Syftet med denna studie är att undersöka vilka skillnader i skogsstrukturer det finns mellan olika skogstyper i Östergötland, samt att diskutera hur dessa skillnader kan påverka den biologiska mångfalden. De skogstyper vi besökt är; hyggen, skogar som är ungefär 40 år gamla, avverkningsanmälda skogar samt naturskogar som är bevarade som naturreservat. De skogsstrukturer vi undersökt är; trädarter, diameter på träd och död ved, nedbrytningsstadie på död ved, spår av brand samt antal håligheter i träd och död ved. Vår studie visade att alla skogstyper hade åtminstone någon av de strukturer som kännetecknar en skog med höga naturvärden och därmed högre biologisk mångfald. Naturreservat var den skogstyp som hade flest skogsstrukturer som kännetecknar skogar med höga naturvärden. Detta tyder på att de hade en större biologisk mångfald gentemot de andra skogstyperna. Hyggen var den skogstyp som uppvisade minst skogsstrukturer som kännetecknar skogar med höga naturvärden vilket tyder på att den biologiska mångfalden var minst på hyggen. Syftet med studien är även att utforma en skoluppgift som liknar vår studie men är anpassad till biologiundervisningen i gymnasieskolan. För att göra detta har vi valt att återskapa vårt arbete som ett biologiprojekt för gymnasiekursen Biologi 1. Detta biologiprojekt innefattar en exkursion med förberedelser och efterarbete.

  • 342.
    Danielsson, Olof
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för neuro- och inflammationsvetenskap. Linköpings universitet, Medicinska fakulteten.
    The Clinical and Pathological Spectrum of Idiopathic Inflammatory Myopathies: Implications for pathogenesis, classification and diagnosis2016Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Background: Idiopathic inflammatory myopathies (IIM) constitute a heterogeneous group of diseases with severe consequences for the life of affected patients. Dermatomyositis, polymyositis and inclusion body myositis (IBM) are the classical representatives of this group. The treatments given today often have limited effects, and are taken at the cost of side effects. Major obstacles in the search for more effective treatments are; (1) an incomplete understanding of the disease mechanisms, (2) difficulties to delineate homogeneous disease groups for clinical studies and (3) the sometimes challenging task to diagnose these diseases.

    Aims: We addressed a number of “loose ends” in the areas of pathogenesis, classification and diagnosis; mechanisms of muscle fiber degeneration in IIM, with a focus of programmed cell death (apoptosis) and invasion of muscle  fibers by inflammatory cells (partial invasion); protecting and mediating factors present in muscle; the association of other diseases with IIM, in particular celiac disease ; the evaluation of two classification systems and laboratory methods for increased diagnostic performance.

    The studies: We included 106 patients, diagnosed at the Neuromuscular unit in Linköping, Sweden, with pathological muscle findings consistent with IIM. The incidence in the county of Östergötland (during 5 years) was 7.3 per million/year (3 patients each year). Of 88 patients with confirmed IIM 4 (4.5 %) had celiac disease, 33 (38%) had an associated systemic inflammatory disease and 5 (5.7 %) had a malignancy. Ninety-nine patients were included for a comparison of two classification systems using criteria of the European Neuromuscle Centre (Amato/ENMC), and the widely used Bohan and Peter classification, both with the addition of IBM according to Griggs et al. Using the Amato/ENMC criteria the most prevalent diagnostic group after IBM (30%) was nonspecific myositis (23%), followed by polymyositis (20%) and dermatomyositis 17%). A substantial number of patients meeting Bohan and Peter (or Griggs) criteria were excluded by Amato/ENMC criteria, most (21/23) due to lack of detectable muscle weakness. Extended muscle sectioning increased the sensitivity of a muscle biopsy by 15 % and the specificity by 22%, and showed an overlap between disease groups. Muscle biopsies from patients with IIM and controls were used to investigate pathological findings considered specific for disease groups, and for the presence of programmed cell death (apoptosis) and disease protecting and mediating factors in muscle. The presence of apoptotic muscle fiber nuclei was detected in muscle with partial invasion (however not in the invaded fibers) in the presence of granzyme B and CD8+ cytotoxic T cells. The major apoptosis inhibiting protein Bcl-2 was shown to be constitutionally expressed in healthy muscle but weakened in IIM.

    Conclusion: We present apoptosis as a possible disease mechanism in parallel with partial invasion of fibers. Furthermore, partial invasion may not be a suitable distinguishing feature in the pathogenesis, or for classification and diagnosis of IIM. We also introduce the anti-apoptotic Bcl-2 as a possible relevant muscle fiber protecting factor. A more extensive pathological work-up improves classification and diagnosis of IIM. The proposed Amato/ENMC creates a substantial portion of patients with non-specific or unclassified myositis. Associated diseases are common in IIM, and also include celiac disease.

    Delarbeten
    1. Classification and Diagnostic Investigation in Inflammatory Myopathies: A Study of 99 Patients
    Öppna denna publikation i ny flik eller fönster >>Classification and Diagnostic Investigation in Inflammatory Myopathies: A Study of 99 Patients
    2013 (Engelska)Ingår i: Journal of Rheumatology, ISSN 0315-162X, E-ISSN 1499-2752, Vol. 40, nr 7, s. 1173-1182Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Objective. Insights into the pathogenesis of inflammatory myopathies have led to new diagnostic methods. The aims of our study were (1) to evaluate the consequences of using the classification of Amato/European Neuromuscular Centre Workshop (ENMC), compared to that of Bohan and Peter; and (2) to evaluate any diagnostic benefit in using an extended pathological investigation. less thanbrgreater than less thanbrgreater thanMethods. From a consecutive retrospective database, we evaluated 99 patients for classification. Patients with inclusion body myositis (IBM) were classified according to Griggs, et al. In addition to routine stainings and immunohistochemistry, a multilevel serial sectioning procedure was performed on paraffin-embedded material, to identify scarce pathological findings. less thanbrgreater than less thanbrgreater thanResults. Classification according to Bohan and Peter could be performed for 83 of the 99 patients, whereas only 60 patients met the Amato/ENMC criteria, the latter resulting in the following diagnostic groups: IBM (n = 18), nonspecific myositis (n = 14), polymyositis (n = 12), dermatomyositis (n = 10), dermatomyositis sine dermatitis (n = 5), and immune-mediated necrotizing myopathy (n = 1). Most of the Amato/ENMC diagnostic groups harbored patients from several of the Bohan and Peter groups, which included a substantial group lacking proximal muscle weakness. The serial sectioning procedure was essential for classification of 9 patients (15%), and led to a more specific diagnosis for 13 patients (22%) according to Amato/ENMC. less thanbrgreater than less thanbrgreater thanConclusion. The classification of Amato/ENMC was more restrictive, forming groups based on clinical criteria and specified myopathological findings, which clearly differed from the groups of the Bohan and Peter classification. An extended pathological investigation increased the diagnostic yield of a muscle biopsy and highlights the quantity and specificity of certain pathological findings.

    Ort, förlag, år, upplaga, sidor
    Journal of Rheumatology, 2013
    Nyckelord
    INFLAMMATORY MYOPATHIES, IDIOPATHIC INFLAMMATORY MYOPATHIES, POLYMYOSITIS, DERMATOMYOSITIS, INCLUSION BODY MYOSITIS
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-96992 (URN)10.3899/jrheum.120804 (DOI)000321993800023 ()
    Anmärkning

    Funding Agencies|University Hospital Linkoping||County Council of Ostergotland||

    Tillgänglig från: 2013-09-02 Skapad: 2013-09-02 Senast uppdaterad: 2020-01-16
    2. Expression of apoptosis related proteins in normal and diseased muscle: A possible role for Bcl-2 in protection of striated muscle
    Öppna denna publikation i ny flik eller fönster >>Expression of apoptosis related proteins in normal and diseased muscle: A possible role for Bcl-2 in protection of striated muscle
    2009 (Engelska)Ingår i: NEUROMUSCULAR DISORDERS, ISSN 0960-8966, Vol. 19, nr 6, s. 412-417Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    The unique absence of major histocompatibility complex class I antigen (MHC-I) expression in normal muscle is one possible mechanism protecting striated muscle. In order to define their possible involvement in protection of normal muscle. we investigated the expression of molecules involved in muscle fibre death and survival mechanisms (Bcl-2, Fas, Fas-ligand and TRAIL), focusing on disorders with possible involvement of cytotoxic T cells. We studied muscle biopsies from 20 healthy volunteers, from 10 patients affected by polymyositis and 10 by Duchenne muscular dystrophy. By using immunohistochemistry, Western blot and real-time PCR we detected a constitutional expression of Bcl-2 in healthy muscle, whereas the expression was weaker in disease processes. Fas-L and TRAIL were not detected in muscle fibres, and Fas only in muscle affected by disease. Our findings indicate that the major apoptotic protein Bcl-2 might have a hitherto unrecognized role in the protection of normal muscle.

    Nyckelord
    Inflammatory myopathy, Apoptosis, Bcl-2, TRAIL, Fas and Fas-L
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-19795 (URN)10.1016/j.nmd.2009.03.008 (DOI)
    Tillgänglig från: 2009-08-10 Skapad: 2009-08-10 Senast uppdaterad: 2020-01-16
  • 343.
    Darkins, Christopher Luke
    et al.
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan. Loughborough University, UK.
    Mandenius, Carl-Fredrik
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.
    Design of large-scale manufacturing of induced pluripotent stem cell derived cardiomyocytes2014Ingår i: Chemical engineering research & design, ISSN 0263-8762, E-ISSN 1744-3563, Vol. 92, nr 6, s. 1142-1152Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    A new approach for design of large-scale manufacture of stem cell derived cells by using the biomechatronic methodology and computer-aided-design tools is described. The systematic conceptual design methodology for systems composed of active mechanical, electronic and biological components, here referred to as biomechatronics, is combined with the methodology for computer-aided design of bioprocesses. The objective has been to systematically investigate and compare by the combination of the methodologies what are favourable design alternatives in terms of equipment configuration and economic parameters. A demonstration case has been used for the manufacture of cardiomyocytes from human induced pluripotent stem cells. The results show how certain configurations are more favourable than others under given boundary conditions. The study indicates that the approach is possible to apply on other related bio-manufacturing systems.

  • 344.
    Davies, Anna C.
    et al.
    School of Clinical Veterinary Science, University of Bristol, UK.
    Nicol, Christine J.
    School of Clinical Veterinary Science, University of Bristol, UK.
    Persson, Mia
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska högskolan.
    Radford, Andrew N.
    School of Biological Sciences, University of Bristol, UK.
    Behavioural and Physiological Effects of Finely Balanced Decision-Making in Chickens2014Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, nr 10, s. e108809-Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In humans, more difficult decisions result in behavioural and physiological changes suggestive of increased arousal, butlittle is known about the effect of decision difficulty in other species. A difficult decision can have a number ofcharacteristics; we aimed to monitor how finely balanced decisions, compared to unbalanced ones, affected the behaviourand physiology of chickens. An unbalanced decision was one in which the two options were of unequal net value (1 (Q1) vs.6 (Q6) pieces of sweetcorn with no cost associated with either option); a finely balanced decision was one in which theoptions were of equal net value (i.e. hens were "indifferent" to both options). To identify hens’ indifference, a titrationprocedure was used in which a cost (electromagnetic weight on an access door) was applied to the Q6 option, to find theindividual point at which hens chose this option approximately equally to Q1 via a non-weighted door. We then comparedbehavioural and physiological indicators of arousal (head movements, latency to choose, heart-rate variability and surfacebody temperature) when chickens made decisions that were unbalanced or finely balanced. Significant physiological (heartratevariability) and behavioural (latency to pen) differences were found between the finely balanced and balancedconditions, but these were likely to be artefacts of the greater time and effort required to push through the weighted doors.No other behavioural and physiological measures were significantly different between the decision categories. We suggestthat more information is needed on when best to monitor likely changes in arousal during decision-making and that futurestudies should consider decisions defined as difficult in other ways.

  • 345.
    De Frenne, Pieter
    et al.
    Forest & Nature Lab, Ghent University, Geraardsbergsesteenweg 267, BE-9090 Gontrode-Melle, Belgium;.
    Brunet, Jörg
    Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, Box 49, SE-230 53 Alnarp, Sweden.
    Decocq, Guillaume
    EDYSAN (FRE 3498 CNRS-UPJV), Universite de Picardie Jules Verne, 1 rue des Louvels, FR-80037 Amiens Cedex, France.
    J. Graae, Bente
    Department of Biology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.
    Hagenblad, Jenny
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten.
    Hermy, Martin
    Forest, Nature & Landscape, K.U. Leuven Celestijnenlaan 200E, BE-3001, Leuven, Belgium.
    Kolb, Annette
    Vegetation Ecology & Conservation Biology, FB2, University of Bremen, Leobener Str., DE-28359 Bremen, Germany.
    H. Lemke, Isgard
    Vegetation Ecology & Conservation Biology, FB2, University of Bremen, Leobener Str., DE-28359 Bremen, Germany.
    Ma, Shiyu
    Forest & Nature Lab, Ghent University, Geraardsbergsesteenweg 267, BE-9090 Gontrode-Melle, Belgium.
    Orczewska, Anna
    Department of Ecology, Faculty of Biology & Environmental Protection, University of Silesia, ul. Bankowa 9, PL-40-007 Katowice, Poland.
    Plue, Jan
    Department of Physical Geography Stockholm University, SE-106 91 Stockholm, Sweden.
    Vranckx, Guy
    Plant Conservation & Population Biology, K.U. Leuven, Kasteelpark Arenberg 31, Box 2435, BE-3001 Leuven, Belgium.
    Wulf, Monika
    Institute of Land Use Systems, Leibniz-ZALF, Eberswalder Strasse 84, DE-15374 M€uncheberg, Germany.
    Verheyen, Kris
    Forest & Nature Lab, Ghent University, Geraardsbergsesteenweg 267, BE-9090 Gontrode-Melle, Belgium.
    Biological Flora of the British Isles: Milium effusum2017Ingår i: Journal of Ecology, ISSN 0022-0477, E-ISSN 1365-2745, Vol. 105, nr 3, s. 839-858Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    1. This account presents information on all aspects of the biology of Milium effusum L. (Wood Millet)

    that are relevant to understanding its ecological characteristics and behaviour. The main topics

    are presented within the standard framework of the Biological Flora of the British Isles: distribution,

    habitat, communities, responses to biotic factors, responses to environment, structure and physiology,

    phenology, floral and seed characters, herbivores and disease, history, and conservation.

    2. The grass Milium effusum is a common species of mature woodland in central and southern England,

    but is less common in the wetter parts of northern England, Wales, Scotland and Ireland. Worldwide,

    the species is native to many temperate, boreal, subarctic and subalpine parts of the northern

    hemisphere: from eastern North America across most of Europe (excluding Mediterranean climates) to

    the Ural Mountains and Black Sea, extending eastwards to the Himalaya, Korea and Japan.

    3. Wood Millet is a shade-tolerant, relatively tall grass (up to 18 m) producing up to 700 caryopses

    per individual. It is characteristic of temperate deciduous woodland, but can also occur in other

    woodland and forest types and even in scrub, alpine meadows, along railways and roads, and on

    rocks. In woods, it is one of the most conspicuous plants of the herb layer in the early summer after

    the disappearance of spring flowering species. While the species is generally considered an ancient

    woodland indicator in England and western Europe, it is also known to colonize secondary, postagricultural

    forests relatively rapidly in other areas such as Denmark, southern Sweden and Poland.

    4. The species has a wide amplitude in terms of soil acidity and nutrient availability, but predominantly

    grows on soils of intermediate soil fertility and soil pH and with high organic matter concentration.

    However, M. effusum can tolerate large quantities of tree-leaf litter on the forest floor and is

    able to grow on very acidic soils.

    5. Changes in land use, climate, densities of large herbivores and atmospheric deposition of nitrogen

    are having effects on populations of Wood Millet. Significant responses of the life-history traits and

    population characteristics have been detected in response to environmental variation and to experimental

    treatments of temperature, nutrients, light and acidity. In many of its habitats across its range,

    M. effusum is currently becoming more frequent. During the last century, its mean elevation of

    occurrence in upland areas of Europe has also increased by several hundreds of metres. Typically,

    management actions are directed towards the conservation of its main habitat type (e.g. ancient

    woodlands of the Milio-Fagetum association) rather than to the species specifically.

  • 346.
    de Paz, Paulino
    et al.
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology, University of León, León, Spain.
    Alvarez-Rodriguez, Manuel
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León.
    Nicolas, Manuel Aguilar
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León.
    Alvarez, Mercedes
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León.
    Chamorro, Ca
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León.
    Borragán, Santiago
    Cabárceno Park, Cantabria, Spain.
    Martinez-Pastor, Felipe
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology, University of León, León, Spain.
    Anel, Luis
    ITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, León.
    Optimization of glycerol concentration and freezing rate in the cryopreservation of ejaculate from brown bear (Ursus arctos).2012Ingår i: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 47, nr 1, s. 105-112Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In order to establish a semen bank for the endangered Cantabrian brown bear, we tested five glycerol concentrations and three freezing rates for electroejaculated semen. Electroejaculation was performed on nine males. Semen was diluted in TES-Tris-Fructose (20% egg yolk, 2% EDTA, 1% Equex) with 2%, 4%, 6%, 8% or 10% glycerol and frozen at -10, -20 or -40°C/min. Before and after cryopreservation, samples were analysed for motility (CASA), viability and acrosomal status (flow cytometry). Pre-freezing results showed that glycerol concentration had no significant effect on total motility or progressive motility, but it significantly decreased VCL, ALH, viability and acrosomal status (p < 0.05). After thawing, sperm motility was higher at extender with 4%, 6% and 8% glycerol, but only at 4% and 6% glycerol for viability and acrosomal status. For 4% and 6% glycerol, freezing rates did not have significant effects. The curve fitting gave an estimate of the optimal glycerol concentration, with all the optimal values for every parameter between 6% and 7% glycerol falling. We propose using 6% glycerol and a freezing velocity of -20°C/min for freezing brown bear ejaculated spermatozoa.

  • 347.
    de Paz, Paulino
    et al.
    Department of Molecular Biology, University of León, León, Spain.
    Mata-Campuzano, María
    Department of Molecular Biology, University of León, León, Spain.
    Tizado, Emilio Jorge
    Department of Biodiversity and Environmental Management, University of León, León, Spain.
    Alvarez, Mercedes
    Department of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain.
    Alvarez-Rodríguez, Manuel
    Department of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain.
    Herraez, Paz
    Department of Molecular Biology, University of León, León, Spain.
    Anel, Luis
    Department of Veterinary Medicine, Anatomy and Surgery, University of León, León, Spain.
    The relationship between ram sperm head morphometry and fertility depends on the procedures of acquisition and analysis used.2011Ingår i: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 76, nr 7, s. 1313-1325Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Sperm head morphometry is a parameter in the evaluation of semen that has been associated with fertility in two ways: comparing morphometric measures between predefined groups of fertility; or analyzing morphometric data by multivariate techniques to identify cell populations. We analyzed the morphometry of ram sperm head by three procedures and checked its relationship with male fertility. A Computer-Aided Sperm Morphometric Assessment procedure (CASMA), an image analysis software (NIS-Elements) in combination with an optical microscope (MO-NIS) and this image analysis software in combination with a scanning electron microscope (SEM-NIS) were used. Eight morphometric parameters were assessed: length, width, area, perimeter, ellipticity, form factor, elongation and regularity. We observed significant differences between the morphometric data of sperm head obtained with three study procedures. The CASMA procedure shows the highest values for all parameters and the SEM-NIS procedure the lowest. The analysis of a semen sample, when only the mean of morphometric parameters is used to describe the cell population, is too limited to interpret their fertilizing capacity. It is essential to analyze the complex structure of the samples by defining subpopulations by multivariate methods. With few exceptions, the means of each morphometric parameter differ between the three subpopulations analyzed in each procedure. Only the subpopulations obtained with the MO-NIS procedure showed a significant correlation with male fertility. In short, it is necessary to establish an instrumental standard for the analysis of sperm morphometry to obtain reliable results and we believe that the MO-NIS system presents these basic requirements.

  • 348.
    de Souza Marinho, Thatiany
    et al.
    University of Estado Rio De Janeiro, Brazil.
    Kawasaki, Adriana
    University of Estado Rio De Janeiro, Brazil.
    Bryntesson, Marcus
    Linköpings universitet, Medicinska fakulteten.
    Souza-Mello, Vanessa
    University of Estado Rio De Janeiro, Brazil.
    Barbosa-da-Silva, Sandra
    University of Estado Rio De Janeiro, Brazil.
    Aguila, Marcia B.
    University of Estado Rio De Janeiro, Brazil.
    Mandarim-de-Lacerda, Carlos A.
    University of Estado Rio De Janeiro, Brazil.
    Rosuvastatin limits the activation of hepatic stellate cells in diet-induced obese mice2017Ingår i: Hepatology Research, ISSN 1386-6346, E-ISSN 1872-034X, Vol. 47, nr 9, s. 928-940Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Aim The aim of this study was to investigate the effects of rosuvastatin in a model of diet-induced obesity and non-alcoholic fatty liver disease, with attention to the activation of hepatic stellate cells (HSCs). Method Male C57BL/6 mice received a control diet (C; 10% energy as lipids) or a high-fat diet (HF; 50% energy as lipids) for 12 weeks, followed by 7 weeks of treatment. Group CR received control diet + rosuvastatin; group HFR received high-fat diet + rosuvastatin. Results The HF group showed higher insulin, total cholesterol, triacylglycerol, and leptin levels than the C group, all of which were significantly diminished by rosuvastatin in the HFR group. The HF group had greater steatosis and activated HSCs than the C group, whereas rosuvastatin diminished the steatosis (less 21%, P amp;lt; 0.001) and significantly inhibited the activation of the HSCs in the HFR group compared to the HF group. The sterol regulatory element-binding protein-1 and the peroxisome proliferator-activated receptor (PPAR)-gamma protein expressions were increased in HF animals and reduced after treatment in the HFR group. By contrast, low PPAR-alpha and carnitine palmitoyltransferase-1 expressions were found in the HF group, and were restored by rosuvastatin treatment in the HFR group. Conclusion Rosuvastatin mitigated hepatic steatosis by modulating PPAR balance, favoring PPAR-alpha over PPAR-gamma downstream effects. The effects were accompanied by a diminishing of insulin resistance, the anti-inflammatory adipokine profile, and HSC activation, avoiding non-alcoholic fatty liver disease progression and non-alcoholic steatohepatitis onset in this model.

  • 349.
    Dean, Rebecca
    et al.
    University of Oxford, UK.
    Cornwallis, Charlie K
    University of Oxford, UK.
    Løvlie, Hanne
    University of Oxford, UK; Stockholm University, Sweden.
    Worley, Kirsty
    University of Oxford, UK; University of East Anglia, UK.
    Richardson, David S.
    University of East Anglia, UK.
    Pizzari, Tommaso
    University of Oxford, UK.
    Male reproductive senescence causes potential for sexual conflict over mating2010Ingår i: Current Biology, ISSN 0960-9822, E-ISSN 1879-0445, Vol. 20, nr 13, s. 1192-1196Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The realization that senescence, age-dependent declines in survival and reproductive performance, pervades natural populations has brought its evolutionary significance into sharper focus. However, reproductive senescence remains poorly understood because it is difficult to separate male and female mechanisms underpinning reproductive success. We experimentally investigated male reproductive senescence in feral fowl, Gallus gallus domesticus, where socially dominant males monopolize access to females and the ejaculates of multiple males compete for fertilization. We detected the signal of senescence on multiple determinants of male reproductive success. The effect of age on status was dependent upon the intensity of intrasexual competition: old males were less likely to dominate male-biased groups where competition is intense but were as likely as young males to dominate female-biased groups. Mating and fertilization success declined sharply with male age largely as a result of population-level patterns. These age-dependent declines translated into sexually antagonistic payoffs: old males fertilized more eggs when they were dominant, but this resulted in females suffering a drastic reduction in fertility. Thus, male senescence causes potential for sexual conflict over mating, and the intensity of this conflict is modulated socially, by the probability of old males dominating reproductive opportunities.

  • 350.
    Debatin, K. M.
    et al.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Beltinger, C.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Bohler, T.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Fellenberg, J.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Friesen, C.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Fulda, S.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Herr, I.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Los, Marek Jan
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Scheuerpflug, C.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Sieverts, H.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Stahnke, K.
    GERMAN CANC RES CTR,DIV MOL ONCOL,D-69120 HEIDELBERG,GERMANY.
    Regulation of apoptosis through CD95 (APO-I/Fas) receptor-ligand interaction1997Ingår i: Biochemical Society Transactions, ISSN 0300-5127, E-ISSN 1470-8752, Vol. 25, nr 2, s. 405-410Artikel i tidskrift (Refereegranskat)
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