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  • 51.
    Vagin, Mikhail
    et al.
    Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, Faculty of Science & Engineering.
    Sekretareva, Alina
    Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering. Department of Chemistry, Stanford University, Stanford, USA.
    Ivanov, Ivan Gueorguiev
    Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, Faculty of Science & Engineering.
    Håkansson, Anna
    Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, Faculty of Science & Engineering.
    Iakimov, Tihomir
    Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, Faculty of Science & Engineering. Graphensic AB, Teknikringen 1F, Linköping, Sweden.
    Syväjärvi, Mikael
    Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, Faculty of Science & Engineering. Graphensic AB, Teknikringen 1F, Linköping, Sweden.
    Yakimova, Rositsa
    Linköping University, Department of Physics, Chemistry and Biology, Semiconductor Materials. Linköping University, Faculty of Science & Engineering. Graphensic AB, Teknikringen 1F, Linköping, Sweden.
    Lundström, Ingemar
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Eriksson, Mats
    Linköping University, Department of Physics, Chemistry and Biology, Chemical and Optical Sensor Systems. Linköping University, Faculty of Science & Engineering.
    Monitoring of epitaxial graphene anodization2017In: Electrochimica Acta, ISSN 0013-4686, E-ISSN 1873-3859, Vol. 238, p. 91-98Article in journal (Refereed)
    Abstract [en]

    Anodization of a graphene monolayer on silicon carbide was monitored with electrochemical impedance spectroscopy. Structural and functional changes of the material were observed by Raman spectroscopy and voltammetry. A 21 fold increase of the specific capacitance of graphene was observed during the anodization. An electrochemical kinetic study of the Fe(CN)(6)(3) (/4) redox couple showed a slow irreversible redox process at the pristine graphene, but after anodization the reaction rate increased by several orders of magnitude. On the other hand, the Ru(NH3) (3+/2+)(6) redox couple proved to be insensitive to the activation process. The results of the electron transfer kinetics correlate well with capacitance measurements. The Raman mapping results suggest that the increased specific capacitance of the anodized sample is likely due to a substantial increase of electron doping, induced by defect formation, in the monolayer upon anodization. The doping concentration increased from less than 1 x 10(13) of the pristine graphene to 4-8 x 10(13) of the anodized graphene. (C) 2017 Elsevier Ltd. All rights reserved.

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  • 52.
    Zhybak, Mikael
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Beni, Valerio
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Department of Science, Dublin, Ireland.
    Vagin, Mikhail Y
    Linköping University, Department of Physics, Chemistry and Biology, Chemical and Optical Sensor Systems. Linköping University, The Institute of Technology.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
    Korpan, Yaroslav
    Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
    Copper/Nafion/PANI Nanocomposite as an electrochemical transducer for creatinine and urea enzymatic biosensing2014In: 24th Anniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper (Other academic)
    Abstract [en]

    Chronic Kidney diseases (CKD) affect, to different degrees, ca. 25 million Americans and 19 million Europeans. Monitoring of creatinine and urea levels is of great importance for a correct evaluation of the status of patients and for their treatment. In this paper, we present the development of creatinine and urea enzymatic biosensors, based on a novel ammonium ion-specific Copper/Nafion/Polyanyline (PANI) nanocomposite electrode (Fig. 1A), and suitable for PoC and decentralised diagnostic applications. . Studies on the nanocomposite electrode revealed its high sensitivity and specificity towards ammonium, in respect to amino acids, creatinine and urea, with response range between 5 and 75 μM (Fig. 1B) and with a detection limit of 1 μM. To demonstrate its suitability as transducer in biosensors, creatinine and urea biosensors were fabricated by immobilising creatinine deiminase or urease, respectively, on the nanocomposite surface. Optimisation of the enzyme immobilisation demonstrated that the incorporation of lactitol markedly improved the stability of the biosensors. The response range of the creatinine biosensor was 2 to 100 μM, which fits well with the normal levels of creatinine in healthy people (30-150 µM).

    The urea biosensor had a response range of 5 to 100 µM. A limit of quantification of 1 µM was achieved for both the biosensors.

    Evaluation of the performance of the biosensors in real sample matrices and cross reactivity studies are currently on-going. We envisage that the proposed design will be particularly compatible with fully-printed systems thus offering a viable route to the mass production of inexpensive sensors for mobile health.

     

     

  • 53.
    Zhybak, M.T.
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
    Beni, Valerio
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Vagin, Mikhail
    Linköping University, Department of Physics, Chemistry and Biology, Chemical and Optical Sensor Systems. Linköping University, Faculty of Science & Engineering.
    Dempsey, Eithne
    Centre for Research in Electroanalytical Technologies, Department of Science, ITT Dublin, Tallaght, Dublin, Ireland.
    Turner, Anthony
    Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering.
    Korpan, Y
    Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine,Kyiv, Ukraine.
    Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite2016In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 77, p. 505-511Article in journal (Refereed)
    Abstract [en]

    The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85±3.4 mA M−1 cm−2 for the creatinine biosensor and 112±3.36 mA M−1 cm−2 for the urea biosensor, with apparent Michaelis–Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1–125 µM, with a limit of detection of 0.5 µM and a response time of 15 s.

    The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

12 51 - 53 of 53
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